Ciita (NM_001243760) Mouse Untagged Clone

CAT#: MC229417

Ciita (untagged) - Mouse class II transactivator (Ciita), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ciita
• Synonyms: C2t; C2ta; EG669998; Gm9475; Mhc2ta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229417 representing NM_001243760
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCACTTCCAGGCCATCCTGGCCCAAGTACAGACACTGCTCTCCAGCCAGAAGCCCAGGCAGGTGC
  GGGCCCTCCTGGATGGCCTGCTGGAAGAAGAGCTGCTCTCACGGGAATACCACTGTGCCTTGCTGCATGA
  GCCTGATGGTGATGCCCTGGCCCGGAAGATTTCCCTGACCCTGCTGGAGAAAGGGGACTTAGACTTGACT
  TTCTTGAGCTGGGTCTGCAACAGTCTGCAGGCTCCCACGGTAGAGAGGGGCACCAGCTACAGGGACCATG
  GAGACCATAGTCTGTGTGCCACCATGGATCTGGGATCTCCAGAGGGCAGCTACCTGGAACTCCTTAACAG
  TGATGCCGACCCCCTACATCTCTACCACCTCTATGACCAGATGGACCTGGCTGGGGAGGAGGAGATCGAA
  CTCAGCTCAGAGCCAGACACAGATACCATCAACTGCGACCAGTTCAGCAAGCTGTTGCAGGACATGGAAC
  TGGATGAAGAGACCCGGGAGGCCTATGCCAACATTGCGGAACTGGATCAGTACGTGTTCCAGGATACCCA
  GCTCGAGGGCCTGAGCAAGGACCTCTTCAAGCACATTGGAGCAGAGGAAGGCTTTGGTGAGAACATAGAG
  ATCCCTGTAGAAGCAGGACAGAAGCCTCAGAAGAGACGCTTCCCGGAAGAGCATGCTATGGACTCAAAGC
  ACAGGAAGCTAGTGCCCACCTCTAGGACCTCACTGAACTATTTGGATCTCCCCACTGGGCACATCCAGAT
  CTTCACCACTCTGCCCCAGGGACTCTGGCAAATCTCAGGGGCTGGCACAGGTCTCTCCAGTGTCCTAATC
  TACCACGGTGAGATGCCCCAGGTCAACCAAGTGCTCCCTTCAAGCAGCCTCAGTATCCCCAGTCTCCCCG
  AGTCCCCAGACCGGCCTGGCTCCACCAGCCCCTTCACACCATCTGCAGCTGACCTGCCCAGCATGCCCGA
  ACCTGCGCTGACCTCCCGTGTAAATGAGACAGAGGACACATCTCCCTCCCCATGCCAAGAGGGTCCCGAG
  TCTTCCATCAAGCTTCCAAAATGGCCAGAGGCTGTGGAGCGATTCCAGCACTCCCTACAGGACAAATACA
  AGGCATTGCCCCAGAGCCCAAGGGGTCCTCTGGTGGCCGTGGAGCTGGTACGGGCCAGGCTGGAAAGAGG
  CAGCAACAAGAGCCAGGAAAGGGAGCTGGCCACTCCCGACTGGACAGAGCGCCAGCTAGCCCACGGTGGT
  CTGGCAGAGGTACTTCAGGTTGTCAGTGACTGCAGGCGACCAGGAGAGACACAGGTGGTCGCTGTGCTGG
  GCAAGGCTGGCCAGGGAAAGAGCCACTGGGCCAGGACAGTGAGTCACACCTGGGCATGTGGCCAGTTGCT
  ACAATATGACTTTGTCTTCTATGTCCCCTGTCATTGCTTGGATCGTCCCGGGGACACCTACCACCTGCGG
  GATCTGCTCTGTCCCCCGAGCCTGCAGCCACTGGCCATGGATGACGAGGTCCTTGATTATATCGTGAGGC
  AGCCAGACCGTGTTCTGCTCATCCTAGATGCTTTCGAGGAGCTAGAGGCCCAAGATGGCCTCCTGCACGG
  ACCCTGTGGATCTCTGTCCCCAGAGCCCTGCTCCCTCCGAGGACTGCTGGCTGGGATCTTCCAGCGGAAG
  CTACTGCGAGGCTGCACACTGCTCCTCACAGCCCGGCCCCGGGGCCGCCTGGCTCAGAGCCTGAGCAAGG
  CAGATGCCATCTTTGAGGTGCCCAGCTTCTCTACCAAGCAGGCCAAGACTTACATGAGGCACTACTTTGA
  GAACTCAGGGACAGCGGGGAACCAAGACAAGGCCCTGGGCCTCCTGGAGGGCCAGCCTCTTCTCTGCAGC
  TATAGTCACAGCCCTGTTGTGTGCAGGGCTGTGTGCCAGCTCTCCAAGGCCCTGCTAGAACAGGGCACAG
  AGGCCCAGCTACCTTGTACACTTACAGGACTCTATGTCAGCCTGCTAGGTCCTGCAGCTCAGAACAGTCC
  TCCCGGAGCCTTAGTCGAGCTGGCCAAGCTGGCCTGGGAGCTGGGACGAAGACACCAAAGCACCTTGCAA
  GAAACCCGGTTTTCATCCGTGGAGGTGAAAACCTGGGCAGTGACCCAAGGCTTGATGCAGCAGACCCTGG
  AGACCACGGAGGCTCAACTGGCCTTCTCCAGTTTTCTGCTACAGTGTTTCCTGGGTGCTGTGTGGCTGGC
  ACAGTGCAATGAAATCAAAGACAAGGAGCTGCCACAGTACCTGGCCTTGACTCCGAGGAAGAAGAGACCC
  TATGACAACTGGCTGGAGGGTGTACCACGCTTTCTGGCTGGATTAGTTTTCCAGCCTCGAGCCCACTGCC
  TGGGAGCTCTGGTGGAGCCTGCAGTGGCTGCAGTGGCGGATAGGAAACAGAAGGTTCTTACCAGGTACCT
  GAAGCGCCTGAAGCTGGGGACACTCCGGGCAGGGAGGCTGCTGGAGCTGCTCCACTGTGCCCACGAGACA
  CAGCAACCTGGGATATGGGAGCATGTTGCACACCAGCTCCCTGGCCACCTCTCCTTCCTGGGCACCCGGC
  TCACACCCCCAGATGTGTATGTGCTGGGCAGGGCCTTGGAGACAGCCAGCCAGGACTTCTCCTTGGACCT
  TCGTCAGACTGGCGTTGAGCCTTCTGGACTGGGAAACCTCGTGGGACTCAGCTGTGTCACCAGTTTCAGG
  GCCTCCTTGAGTGATACAATGGCATTATGGGAGTCCCTTCAGCAGCAGGGAGAAGCCCAGCTACTCCAGG
  CGGCAGAGGAGAAGTTCACCATTGAGCCATTTAAAGCCAAATCCCCAAAGGATGTGGAAGACCTGGATCG
  TCTCGTGCAGACCCAGAGGCTGAGAAACCCCTCAGAAGATGCAGCCAAGGATCTTCCTGCCATCCGGGAC
  CTTAAGAAGCTAGAGTTTGCGTTGGGCCCCATCTTGGGCCCCCAGGCTTTCCCCACACTGGCAAAGATCC
  TTCCAGCCTTCTCTTCTCTGCAACACCTGGACCTGGACTCACTTAGTGAGAACAAGATCGGAGACAAGGG
  TGTGTCGAAGCTCTCAGCCACCTTCCCTCAGCTGAAGGCCCTGGAGACGCTCAACTTGTCCCAAAACAAC
  ATCACTGATGTGGGTGCCTGCAAGCTTGCAGAAGCTCTGCCAGCCCTAGCCAAGTCCCTCCTAAGGCTGA
  GCTTGTACAATAACTGCATCTGTGACAAAGGAGCCAAGAGCCTGGCACAAGTACTTCCGGACATGGTGTC
  CCTGCGTGTGATGGATGTCCAGTTCAACAAGTTCACGGCTGCCGGTGCCCAGCAACTGGCCTCCAGCCTT
  CAGAAGTGCCCTCAGGTGGAAACACTGGCAATGTGGACACCCACTATCCCCTTTGGGGTTCAGGAACACC
  TGCAGCAGCTGGATGCCAGGATCAGTCTGAGATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001243760
• Insert Size: 3465 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001243760.2, NP_001230689.1
• RefSeq Size: 5435 bp
• RefSeq ORF: 3465 bp
• Locus ID: 12265
• Cytogenetics: 16 A1
• Gene Summary: This gene encodes a member of the NOD-like receptor protein family. This protein acts as a transcriptional coactivator and component of the enhanceosome complex to stimulate transcription of MHC class II genes in the adaptive immune response. This protein may also regulate the transcription of MHC class I genes. Mutations in the human gene have been linked to a rare immunodeficiency, bare lymphocyte syndrome, and homozygous knockout mice exhibit many features of this disease. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]
  Transcript Variant: This variant (1) uses an alternate in-frame splice site in the 5' coding region, compared to variant 4. It encodes isoform 1, which is shorter than isoform 4.