Atp11a (NM_001293667) Mouse Untagged Clone

CAT#: MC229407

Atp11a (untagged) - Mouse ATPase, class VI, type 11A (Atp11a), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Atp11a
• Synonyms: 4930558F19Rik; Atpc1h; AU040868; Ih
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229407 representing NM_001293667
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTGCAGTCTATTGAGGACGCTCGTGCGCAGATACTGTGCAGGGGAAGAGAACTGGGTGGACAGTC
  GGACCATCTACGTGGGACACAAGGAGCCCCCTCCGGGTGCAGAGGCCTACATTCCACAGAGATACCCCGA
  CAATAGAATCGTCTCCTCCAAGTACACATTCTGGAACTTCATACCCAAGAACTTATTTGAACAGTTCAGA
  AGAATAGCCAACTTTTATTTCCTCATCATCTTCCTGGTACAGTTGATCATCGACACACCCACAAGTCCAG
  TGACAAGCGGGCTCCCACTCTTCTTCGTCATCACTGTCACGGCCATCAAGCAGGGCTATGAAGACTGGCT
  TCGCCACAAGGCCGACAACGCCATGAACCAGTGTCCCGTGCACTTTATCCAGCATGGCAAGCTGGTCCGC
  AAGCAGAGTCGGAAGCTGAGGGTTGGGGACATTGTCATGGTGAAGGAAGATGAGACCTTTCCCTGTGACC
  TGATCTTTCTCTCCAGCAACCGGGCAGATGGGACATGCCATGTCACTACAGCCAGCTTAGACGGAGAGTC
  GAGCCATAAAACTCACTACGCAGTGCAGGATACCAAGGGCTTCCACACAGAGGCGGATGTTGACAGCTTG
  CACGCCACGATCGAGTGTGAACAGCCACAGCCTGACCTCTACAAGTTTGTGGGGCGCATCAATGTTTACA
  ACGACCTGAACGACCCTGTAGTGAGGCCTTTGGGGTCAGAAAACCTGCTTCTCAGAGGAGCCACACTCAA
  AAACACAGAGAAGATCTTTGGTGTGGCTATCTACACAGGCATGGAGACCAAGATGGCCCTGAACTATCAA
  TCCAAGTCCCAAAAGAGATCTGCTGTGGAAAAGTCAATGAACACATTCCTGATCGTGTACCTCTGCATCC
  TGGTGAGCAAGGCCCTGATCAACACGGTGCTGAAGTACGTGTGGCAGAGTGAGCCCTTCCGAGATGAGCC
  ATGGTACAACGAGAAGACCGAATCCGAGCGCCAGAGGAATTTGTTCCTCAGGGCCTTCACCGACTTCCTG
  GCCTTCATGGTCCTCTTCAATTACATCATCCCCGTATCCATGTACGTCACCGTGGAGATGCAGAAGTTCC
  TCGGCTCCTACTTCATCACCTGGGATGAGGACATGTTTGATGAGGAAATGGGGGAAGGGCCTCTGGTCAA
  CACATCTGATCTGAATGAGGAGTTGGGACAGGTGGAATACATCTTCACTGACAAGACCGGCACGCTTACA
  GAGAACAACATGGCCTTCAAGGAGTGCTGCATCGAGGGCCATGTCTACGTACCCCATGTCATCTGCAATG
  GGCAGGTCCTTCCCGACTCTTCTGGCATTGACATGATCGATTCTTCCCCCGGAGTCTGTGGAAGGGAACG
  GGAGGAGCTGTTTTTCAGGGCCATCTGCCTGTGCCACACTGTCCAAGTGAAAGATGACCATTGTGGGGAT
  GATGTCGATGGTCCCCAGAAATCTCCAGATGCAAAATCCTGTGTGTACATATCGTCCTCGCCTGATGAGG
  TTGCACTGGTCGAAGGCGTGCAGAGGCTTGGATTCACGTACCTGAGGCTGAAGGACAATTACATGGAAAT
  ACTGAACAGGGAGAACGACATAGAGAGATTTGAATTGCTAGAAGTGCTGACCTTTGATTCTGTGCGGAGA
  AGAATGAGTGTGATTGTAAAGTCTACTACAGGAGAAATTTATCTGTTTTGCAAAGGAGCAGATTCTTCAA
  TATTTCCCCGAGTGATAGAAGGCAAAGTAGACCAGGTCCGGTCTAGAGTGGAGCGCAATGCCGTGGAAGG
  GCTGCGAACCCTGTGTGTTGCATACAAGAGGCTCGAGCCAGAGCAGTACGAAGATGCTTGCCGACTGCTA
  CAGAGTGCCAAAGTGGCGCTTCAGGACCGGGAGAAGAAGCTGGCAGAAGCCTACGAGCAGATAGAGAAGG
  ACCTTGTTCTGCTTGGTGCTACGGCTGTTGAGGACAGGCTCCAGGAGAAAGCTGCTGACACAATTGAGGC
  ACTACAGAAGGCAGGCATCAAGGTCTGGGTGCTCACCGGGGACAAGATGGAGACAGCATCCGCCACCTGC
  TATGCCTGCAAGCTGTTCCGCAGGAGCACGCAGCTGCTGGAGCTGACCACCAAGAAGCTAGAGGAGCAGA
  GCCTGCACGATGTGCTCTTCGACCTGAGCAAGACAGTGCTGCGCTGCAGCGGGAGCATGACCAGGGACTC
  CTTCTCGGGGCTCTCCACAGATATGCACGACTATGGTTTAATCATCGATGGAGCAGCACTGTCCTTGATA
  ATGAAGCCGAGAGAGGACGGGAGCTCCTCGGGCAACTACCGAGAGCTCTTCCTGGAGATCTGCAGGAACT
  GCAGTGCTGTGCTGTGCTGCCGGATGGCACCCTTGCAGAAGGCCCAGATCGTTAAGTTAATCAAATTTTC
  AAAGGAGCATCCTATTACCTTAGCAATTGGTGATGGTGCAAATGATGTCAGTATGATCCTGGAGGCCCAC
  GTGGGCATAGGTGTCATCGGGAAAGAGGGTCGCCAGGCTGCAAGGAACAGCGACTATGCAATACCCAAGT
  TTAAACACTTAAAGAAGATGCTTCTTGTTCATGGGCATTTTTATTATATCAGGATATCTGAGCTTGTGCA
  ATACTTCTTTTATAAGAACGTCTGCTTCATTTTCCCTCAGTTTTTGTACCAGTTCTTCTGCGGATTTTCA
  CAGCAGACTTTGTACGATACTGCGTATCTGACTCTCTACAACATCAGCTTCACTTCCCTCCCGATTCTCC
  TGTACAGCCTCATGGAGCAGCACGTGGGCATTGACGTGCTCAAGAGAGACCCGACCCTCTACAGAGACAT
  TGCCAAGAATGCGCTGCTGCGCTGGCGGGTGTTCATTTACTGGACGTTTCTCGGCGTTTTTGACGCTTTG
  GTATTTTTCTTTGGTGCTTATTTCATATTTGAGAACACAACCGTGACCATCAACGGACAGATGTTTGGGA
  ACTGGACCTTCGGGACGCTGGTGTTCACTGTGATGGTGCTCACTGTCACACTGAAGCTCGCACTGGACAC
  GCACTACTGGACTTGGATCAACCACTTTGTCATCTGGGGGTCGCTGCTCTTCTACATTGCCTTCTCCCTG
  CTCTGGGGAGGGGTTATCTGGCCGTTCCTCAGTTACCAGAGGATGTACTATGTGTTCATATCCATGCTGT
  CCAGTGGGCCTGCCTGGCTGGGTATCATACTGCTTGTCACGGTCGGCCTCCTCCCTGACGTCCTCAAGAA
  GGTCCTGTGTAGGCAGCTGTGGCCCACGGCAACGGAGAGAACTCAGCAGAGCACCCGACTCAAAGCATTG
  GCCGATGCCACCTCGAACAGCGCCAGGCCCCTGCTGAAGGACTTCTTACCGCAGCCAAGACATGTCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001293667
• Insert Size: 3429 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001293667.1, NP_001280596.1
• RefSeq Size: 7547 bp
• RefSeq ORF: 3429 bp
• Locus ID: 50770
• Cytogenetics: 8 A1.1
• Gene Summary: Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) contains an alternate exon in the 3' end compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1.