Bag6 (NM_001252468) Mouse Untagged Clone

CAT#: MC229405

Bag6 (untagged) - Mouse BCL2-associated athanogene 6 (Bag6), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Bag6
• Synonyms: 2410045D21Rik; AA408914; BAG-6; Bat3; D17H6S52E; G3; Scythe
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229405 representing NM_001252468
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCGAGTGATAGTGCCAGTACCGCTATGGAGGAGCCTGACAGCCTGGAGGTACTGGTGAAGACCC
  TGGACTCTCAGACTCGGACTTTTATTGTGGGGGCCCAGATGAATGTAAAGGAGTTTAAGGAACACATAGC
  TGCCTCTGTCAGCATCCCTTCCGAGAAACAGCGGCTCATCTACCAGGGCCGGGTCCTACAAGACGACAAG
  AAGCTCCAGGAGTACAACGTTGGGGGAAAGGTTATTCACCTGGTGGAACGGGCTCCTCCTCAGACTCAGC
  TTCCTTCTGGAGCATCTTCTGGGACAGGCTCTGCCTCAGCAACTCATGGTGGGGCACCCCTGCCTGGCAC
  TCGGGGCCCTGGGGCTTCTGTTCATGACCGGAATGCCAACAGCTATGTCATGGTTGGAACCTTCAATCTT
  CCTAGTGAGCCCCGGGTCCGGCTGGTGATGGCTCAGCACATGATTAGGGATATACAGACCTTACTGTCCC
  GGATGGAGTGTCGAGGGGGAACCCAAGCTCAGGCCAGTCAGCCACCCCCGCAGACACCGCAGACTGTGGC
  CTCAGAGACGGTAGCCTTGAACTCACAAACATCAGAACCAGTCGAAAGTGAAGCTCCTCCTCGAGAGCCC
  ATGGAGTCAGAAGAAATGGAGGAACGTCCCCCAACCCAGACTCCAGAGCTTGCGCCCTCAGGCCCAGCTC
  CCGCAGGCCCAGCTCCCGCAGGCCCAGCTCCCGCGCCAGAGACAAATGCACCCAACCACCCTTCCCCCGC
  AGAGCACGTGGAGGTGCTCCAGGAGCTTCAGCGCTTGCAGCGCCGTCTTCAGCCTTTCCTACAGCGCTAC
  TGCGAGGTCCTGGGCGCTGCTGCCACCACAGACTACAACAACAACCATGAGGGCCGTGAGGAGGACCAGA
  GGTTGATCAACTTGGTTGGGGAGAGCCTGCGGCTACTGGGCAACACTTTCGTGGCATTGTCTGATCTGCG
  CTGCAATCTAGCCTGTGCACCCCCACGGCACCTGCACGTGGTTCGGCCTATGTCTCACTACACGACTCCC
  ATGGTGCTCCAGCAGGCAGCCATTCCCATTCAGATCAATGTGGGGACTACTGTGACCATGACAGGCAATG
  GGGCTCGGCCTCCACCAGCTCCTGGTGCGGAGGCAGCAACCCCAGGTTCTGCCCAGGCCACATCCCTGCC
  TCCCTCTTCCACCACTGTTGATTCATCAACTGAAGGAGCTCCCCCACCAGGGCCAGCACCGCCACCAGCC
  TCCAGCCACCCACGGGTCATCCGGATTTCCCACCAGAGTGTGGAGCCCGTGGTCATGATGCACATGAACA
  TTCAAGATTCTGGAGCACAGCCTGGTGGTGTGCCAAGTGCTCCCACTGGTCCACTGGGACCTCCTGGTCA
  TGGACAGACCCTGGGACAGCAGGTGCCCGGCTTCCCAACAGCACCAACTCGGGTGGTGATTGCCCGGCCA
  ACTCCTCCACAGGCTCGGCCTTCCCATCCTGGGGGACCTCCAGTCTCTGGAGCTCTGCAGGGCGCTGGGC
  TGGGTACAAACACTTCATTGGCCCAGATGGTGAGCGGCCTTGTGGGGCAACTTCTTATGCAGCCTGTTCT
  TGTGGCTCAGGGGACTCCAGGAATGGCTCAGGCTCAGGCCCAGGCCCAGGCTCAGGCCCAGGCCCAGGCC
  CAGGCTCCAGCTCCGGCTCCGGCTCCAGCTCCAGCTCCTGCCACTGCTTCAGCTAGTGCTGGTACTACCA
  ACACAGCTACCACAGCTGGCCCTGCTCCTGGGGGTCCTGCCCAGCCTCCACCTCCTCAGCCCTCTGCAGC
  TGATCTTCAGTTCTCTCAGCTCCTGGGAAATCTGCTGGGGCCTGCAGGGCCAGGGGCTGGGGGGCCAGGC
  ATGGCTTCTCCCACCATCACTGTGGCAATGCCTGGTGTCCCTGCTTTTCTCCAGGGCATGACTGATTTTT
  TGCAGGCATCACAGACTGCCCCTCCACCCCCTCCACCTCCTCCACCCCCACCCCCTGCCCCAGAGCAGCA
  GAGCACACCCCCACCAGGGTCTCCTTCTGGTGGAACAGCAAGCCCTGGAGGCTTAGGTCCTGAGAGCCTG
  CCACCAGAGTTTTTCACCTCAGTGGTGCAGGGCGTGTTGAGCTCCCTCCTGGGCTCCTTGGGGGCTCGAG
  CTGGCAGCAGTGAGAGTATCGCTGCCTTCATCCAACGCCTCAGTGGATCCAGCAACATCTTTGAGCCTGG
  GGCTGATGGCGCCCTTGGATTCTTCGGAGCTCTGCTCTCTCTCCTGTGCCAGAATTTCTCCATGGTGGAT
  GTGGTGATGCTTCTCCATGGCCATTTCCAGCCACTGCAGCGGCTCCAGCCACAGCTGCGATCTTTCTTCC
  ACCAGCACTACCTGGGTGGCCAGGAGCCCACGCCTAGCAACATCCGGATGGCGACCCACACACTGATCAC
  TGGGCTGGAAGAGTATGTAAGGGAGAGTTTTTCTTTGGTACAGGTTCAGCCAGGTGTGGATATCATCCGG
  ACAAATTTAGAATTTCTCCAAGAGCAGTTTAACAGCATTGCTGCTCATGTGCTGCACTGTACAGACAGTG
  GATTTGGAGCCCGGTTGCTGGAGCTGTGTAACCAGGGCCTGTTTGAATGCTTGGCCCTGAACCTCCACTG
  CTTGGGGGGACAGCAAATGGAGCTTGCTGCTGTCATCAATGGTCGAATTCGCCGCATGTCTCGCGGGGTG
  AATCCATCCTTGGTGAGCTGGCTGACAACCATGATGGGACTGAGGCTTCAGGTGGTCTTGGAGCACATGC
  CTGTGGGTCCCGACGCCATCCTCAGATATGTTCGTAGGGTTGGTGATCCTCCTCAGACACTTCCTGAAGA
  GCCGATGGAAGTTCAGGGAGCAGAAAGAACTTCCCCTGAACCTCAGAGAGAGAATGCTTCCCCAGCCCCT
  GGAACAACAGCAGAAGAAGCCATGTCCCGAGGCCCGCCCCCTGCTCCTGAAGGAGGTTCCCGAGATGAAC
  AGGATGGAGCTTCAGCTGATGCAGAACCCTGGGCAGCTGCAGTCCCCCCTGAATGGGTCCCTATTATCCA
  GCAGGACATTCAGAGCCAGCGGAAGGTGAAACCTCAGCCGCCCCTGAGTGATGCCTACCTCAGTGGCATG
  CCTGCCAAGAGACGAAAGACAATGCAGGGTGAGGGCCCCCAGCTGCTACTCTCAGAGGCAGTGAGCCGGG
  CAGCTAAGGCAGCCGGAGCTCGGCCCCTGACAAGCCCCGAGAGCCTGAGCCGGGACCTGGAGGCACCAGA
  GGTTCAGGAGAGCTACAGGCAGCAGCTCCGGTCTGATATCCAAAAACGACTGCAGGAAGATCCCAACTAC
  AGCCCCCAGCGCTTCCCTAATGCCCATCGGGCATTTGCTGATGACCCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001252468
• Insert Size: 3411 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252468.1, NP_001239397.1
• RefSeq Size: 3804 bp
• RefSeq ORF: 3411 bp
• Locus ID: 224727
• Cytogenetics: 17 18.59 cM
• Gene Summary: ATP-independent molecular chaperone preventing the aggregation of misfolded and hydrophobic patches-containing proteins (PubMed:18056262, PubMed:18678708, PubMed:20713601). Functions as part of a cytosolic protein quality control complex, the BAG6/BAT3 complex, which maintains these client proteins in a soluble state and participates to their proper delivery to the endoplasmic reticulum or alternatively can promote their sorting to the proteasome where they undergo degradation (PubMed:20713601). The BAG6/BAT3 complex is involved in the post-translational delivery of tail-anchored/type II transmembrane proteins to the endoplasmic reticulum membrane. Recruited to ribosomes, it interacts with the transmembrane region of newly synthesized tail-anchored proteins and together with SGTA and ASNA1 mediates their delivery to the endoplasmic reticulum. Client proteins that cannot be properly delivered to the endoplasmic reticulum are ubiquitinated by RNF126, an E3 ubiquitin-protein ligase associated with BAG6 and are sorted to the proteasome. SGTA which prevents the recruitment of RNF126 to BAG6 may negatively regulate the ubiquitination and the proteasomal degradation of client proteins. Similarly, the BAG6/BAT3 complex also functions as a sorting platform for proteins of the secretory pathway that are mislocalized to the cytosol either delivering them to the proteasome for degradation or to the endoplasmic reticulum. The BAG6/BAT3 complex also plays a role in the endoplasmic reticulum-associated degradation (ERAD), a quality control mechanism that eliminates unwanted proteins of the endoplasmic reticulum through their retrotranslocation to the cytosol and their targeting to the proteasome. It maintains these retrotranslocated proteins in an unfolded yet soluble state condition in the cytosol to ensure their proper delivery to the proteasome (By similarity). BAG6 is also required for selective ubiquitin-mediated degradation of defective nascent chain polypeptides by the proteasome. In this context, it may participate to the production of antigenic peptides and play a role in antigen presentation in immune response (PubMed:20713601). BAG6 is also involved in endoplasmic reticulum stress-induced pre-emptive quality control, a mechanism that selectively attenuates the translocation of newly synthesized proteins into the endoplasmic reticulum and reroutes them to the cytosol for proteasomal degradation. BAG6 may ensure the proper degradation of these proteins and thereby protects the endoplasmic reticulum from protein overload upon stress (By similarity). By inhibiting the polyubiquitination and subsequent proteasomal degradation of HSPA2 it may also play a role in the assembly of the synaptonemal complex during spermatogenesis (PubMed:18678708). Also positively regulates apoptosis by interacting with and stabilizing the proapoptotic factor AIFM1 (PubMed:18056262). By controlling the steady-state expression of the IGF1R receptor, indirectly regulates the insulin-like growth factor receptor signaling pathway (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an exon in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.