Ptprc (NM_001268286) Mouse Untagged Clone

CAT#: MC229399

Ptprc (untagged) - Mouse protein tyrosine phosphatase, receptor type, C (Ptprc), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ptprc
• Synonyms: B220; Cd45; CD45R; L-CA; loc; Ly-5; Lyt-4; T200
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229399 representing NM_001268286
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCATGGGTTTGTGGCTCAAACTTCTGGCCTTTGGATTTGCCCTTCTGGACACAGAAGTCTTTGTCA
  CAGGGCAAACACCTACACCCAGTGATGCTACCACAACGAAGCAAACATGTGCTGCCATGTTTGGGAACAT
  TACTGTGAATTACACCTATGAATCTAGTAATCAGACTTTTAAGGCAGACCTCAAAGATGTCCAAAATGCT
  AAGTGTGGAAATGAGGATTGTGAAAACGTGTTAAATAATCTAGAAGAATGCTCACAGATAAAAAACATCA
  GTGTGTCTAATGACTCATGTGCTCCAGCTACAACTATAGATTTATATGTACCACCAGGGACTGACAAGTT
  TTCGCTACATGACTGCACACCAAAAGAAAAGGCTAATACTTCAATTTGTTTGGAGTGGAAAACAAAAAAC
  CTTGATTTCAGAAAATGCAACAGTGACAATATTTCATATGTACTCCACTGTGAGCCAGAAAATAATACAA
  AATGCATTAGAAGAAATACATTCATACCTGAAAGATGTCAGTTGGACAACCTTCGTGCCCAAACAAATTA
  CACATGTGTAGCAGAAATCTTATATCGCGGTGTAAAACTCGTCAAAAATGTTATAAATGTGCAGACAGAT
  TTGGGGATTCCAGAAACGCCTAAGCCTAGTTGTGGGGATCCAGCTGCAAGAAAAACGTTAGTCTCTTGGC
  CTGAGCCTGTATCTAAACCTGAGTCTGCATCTAAACCCCATGGATATGTTTTATGCTATAAGAACAATTC
  AGAAAAATGTAAAAGTTTGCCTAATAATGTGACCAGTTTTGAGGTGGAAAGCTTGAAACCTTATAAATAC
  TATGAAGTGTCCCTACTTGCCTATGTCAATGGGAAGATTCAAAGAAATGGGACTGCTGAGAAGTGCAATT
  TTCACACAAAAGCAGATCGTCCGGACAAGGTCAATGGAATGAAAACCTCCCGGCCGACAGACAATAGTAT
  AAATGTTACATGTGGTCCTCCTTATGAAACTAATGGCCCTAAAACCTTTTACATTTTGGTAGTCAGAAGT
  GGAGGTTCTTTTGTTACAAAATACAACAAGACAAACTGTCAGTTTTATGTAGATAATCTCTACTATTCAA
  CTGACTATGAGTTTCTGGTCTCTTTTCACAATGGAGTGTACGAGGGAGATTCAGTTATAAGAAATGAGTC
  AACAAATTTTAATGCTAAAGCACTGATTATATTCCTGGTGTTTCTGATTATTGTGACATCAATAGCCTTG
  CTTGTTGTTTTGTATAAAATCTATGATCTGCGCAAGAAAAGATCCAGCAATTTAGATGAACAACAGGAAC
  TCGTTGAAAGGGATGATGAAAAGCAGCTGATGGATGTGGAGCCAATCCATTCTGACATTTTGTTGGAAAC
  ATACAAAAGGAAGATTGCTGATGAGGGCAGACTGTTCCTGGCTGAATTTCAGAGCATTCCACGGGTATTC
  AGCAAGTTTCCCATCAAAGATGCCCGAAAGCCCCACAATCAGAATAAAAACCGTTATGTTGACATTCTTC
  CCTATGATTATAACCGTGTGGAACTCTCTGAAATAAATGGAGATGCAGGGTCCACCTACATAAATGCCAG
  CTACATTGATGGCTTCAAGGAACCCAGGAAATACATTGCTGCACAAGGGCCCCGGGATGAGACAGTTGAT
  GACTTCTGGAGGATGATCTGGGAGCAAAAGGCCACAGTTATTGTCATGGTCACACGATGTGAAGAAGGAA
  ACAGGAACAAGTGCGCAGAATACTGGCCAAGCATGGAGGAAGGCACTCGGGCTTTCAAAGATATTGTTGT
  GACAATCAATGACCACAAACGATGTCCTGATTACATCATTCAGAAGCTGAACGTTGCACATAAAAAAGAA
  AAAGCAACTGGAAGAGAAGTGACTCATATCCAATTCACCAGCTGGCCAGACCATGGGGTTCCTGAAGACC
  CTCACCTGCTCCTCAAACTTCGACGGAGAGTTAATGCTTTTAGCAACTTCTTCAGTGGTCCCATTGTGGT
  GCACTGCAGTGCTGGTGTTGGGCGTACAGGTACCTACATTGGAATTGATGCCATGCTGGAAGGCCTGGAA
  GCAGAGGGCAAAGTGGATGTCTATGGTTATGTTGTCAAGCTAAGGCGACAGAGGTGTCTGATGGTGCAAG
  TGGAGGCACAGTATATCCTGATTCATCAGGCTTTAGTGGAATACAATCAGTTTGGAGAAACAGAAGTGAA
  CTTGTCTGAGTTACATTCATGCCTACACAACATGAAGAAGAGAGATCCACCCAGTGACCCCTCCCCTCTG
  GAGGCTGAATACCAGAGACTTCCTTCATACAGGAGTTGGAGGACACAGCACATTGGAAATCAAGAAGAAA
  ATAAGAAGAAGAACAGGAATTCTAATGTTGTTCCATATGACTTTAACAGAGTGCCACTTAAGCATGAACT
  GGAGATGAGCAAAGAGAGTGAGCCTGAATCAGATGAGTCTTCAGATGATGACAGTGACTCAGAAGAAACC
  AGCAAATACATTAATGCATCCTTTGTGATGAGTTACTGGAAACCAGAAATGATGATTGCTGCTCAGGGGC
  CACTAAAAGAAACGATCGGTGACTTTTGGCAGATGATATTCCAAAGAAAAGTCAAAGTTATTGTGATGTT
  GACAGAGTTAGTGAATGGAGACCAGGAAGTCTGTGCTCAGTACTGGGGCGAAGGAAAGCAGACTTATGGA
  GACATGGAAGTGGAGATGAAAGACACAAACAGAGCCTCAGCCTACACTCTCCGAACTTTTGAGCTGAGAC
  ATTCCAAGAGGAAGGAGCCCAGAACTGTGTACCAGTACCAGTGTACCACATGGAAAGGGGAAGAGCTGCC
  TGCAGAACCCAAAGACCTGGTGTCTATGATTCAGGACCTCAAACAGAAGCTTCCCAAGGCTTCCCCAGAA
  GGGATGAAGTATCACAAGCATGCATCCATCCTCGTCCACTGCAGAGATGGATCCCAGCAGACAGGGTTGT
  TCTGTGCCTTGTTCAATCTCTTGGAAAGTGCAGAAACAGAAGATGTGGTTGATGTTTTCCAAGTGGTAAA
  GTCTCTACGCAAAGCACGGCCTGGGGTGGTGTGCAGCTATGAGCAATACCAGTTCCTCTATGACATCATC
  GCCAGCATCTATCCCGCCCAGAATGGACAAGTCAAGAAAACAAACAGCCAAGACAAAATTGAATTTCATA
  ATGAAGTGGATGGAGGCAAGCAGGATGCTAACTGTGTCCGTCCAGATGGTCCTCTGAATAAAGCCCAGGA
  AGACAGCAGAGGGGTGGGAACCCCGGAGCCTACCAATAGTGCTGAGGAACCAGAACATGCTGCCAATGGT
  TCTGCGAGCCCAGCTCCAACCCAGAGTTCATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001268286
• Insert Size: 3393 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001268286.1, NP_001255215.1
• RefSeq Size: 5177 bp
• RefSeq ORF: 3393 bp
• Locus ID: 19264
• Cytogenetics: 1 60.73 cM
• Gene Summary: Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN (By similarity). Dephosphorylates LYN, and thereby modulates LYN activity.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks four in-frame exons in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1.