Usp43 (NM_001291049) Mouse Untagged Clone

CAT#: MC229396

Usp43 (untagged) - Mouse ubiquitin specific peptidase 43 (Usp43), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Usp43
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229396 representing NM_001291049
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCCGGGCGTGGGGAACGCACTGGGAGAGGGGCCGCCCGCGCCCCGGCCCCGCCGCCGCCGTTCCC
  TTCGCCGCCTGCTTAACCGCTTCCTGCTAGCTCTGGGCAGCCGCTCCCGCTCGGGGGACTCGCCGCCCCG
  GCCCCCGGCCCAGCCCAGTCCCTACGATGGCGATGGAGAAGGGGGCTTTGCCTGCGCCCCGGGCCCGGCA
  CCAGCCTCTGCCGGGAGTCCAGGGCCAGATCGTCCTCCGGGATCCCAACCCCAGATATCTTCGGGTGACG
  GGGCGCGTCCGCCCGGCGCTCAGGGCTTGAAGAACCATGGCAACACCTGTTTCATGAACGCGGTGGTGCA
  GTGTCTCAGCAACACCGACCTGCTGGCCGAGTTCCTAGCGCTGGGGCGCTACCGGGCGGCGCCGGGCCGC
  GCCGAGGTCACCGAGCAGCTGGCGGCGCTGGTGCGCGCGCTCTGGACGCGCGAATACACGCCCCAACTTT
  CCGCAGAGTTCAAGAATGCTGTTTCTAAGTATGGCTCCCAGTTCCAAGGCAATTCCCAGCACGATGCCCT
  GGAATTCCTGCTCTGGCTTCTGGATCGTGTCCACGAGGACTTGGAAGGCTCAGCTCACGGGCTGGTATCT
  GAGCAGCTGCCCCCAGAAGTGAGTAAGATCTCAGAGGACCTCCGGCCATCTGCAGCTCCTACCTCCTTAG
  GGCCCAGCTTTGTGCAGAGCCACTTTCAAGCACAGTACAGGTCTTCCTTGACTTGTCCACACTGCCTAAA
  ACAAAGCAACACCTTCGACCCTTTCCTGTGTGTGTCCCTTCCCATCCCCTTGCGCCAGACGAGATTCTTG
  AGTGTCACTTTGGTGTTCCCCTCAAAGAGCCAGCGGTTTCTGCGTGTTGGCTTGGCCGTGCCAATCCTCA
  GCACAGTGGCGGCCTTGAGGAAGATGGTTGCAGAGGAAGGAGGTGTCCCTGCAGAGGAGGTGATCTTGGT
  TGAACTGTATCCCAATGGATTCCAGCGGTCATTCTTTGATGAAGAGGACCTGAATACCATTGCAGAGGGT
  GACAATGTGTATGCTTTCCAAGTCCCTCCTTCACCTGGTCTGGGGACGCTCTCAGCCCATCCGTCTGGTC
  TGTCAGTGTCACCGCGCTTGCCAGTCCGTGACAGCCAGCGATTCTCTGGGCCTCTCCACAGCGAGAACAG
  GGTGGTCTTTCTCTTCTGTAACCTGGTGGGCTCAGGGCAGCAGGCGAGCAGGTTTGGACCACCGTTCTTG
  ATTCGGGAAGATAGAACAATCTCCTGGGCCCAGCTTCAACAGTGTATTCTCAGCAAGGTTCGATGCCTCA
  TGAGGAGTGAGGTCTCTGCACAGGACCTGGGGACTCTGTTCTCCATCCGGGTTGTGGGGCTCTCCTTGGC
  CTGCAGCTACTTATCTCCAAAGGACAACCGGCCCCTTTGCCACTGGGCAGTTGACAGGGCTTTGCACCTC
  AGGAGGCCAGGAGGCCCTCCTCATGTCAAGCTGGCTGTGGAATGGGACAGCTCTGTCACAGAGCGCCTGT
  TTGGGAGCCTCCAGGAGGAACGGGTCCAGGATGCAGACAGTGTGTGGCGGCAGCAGCAGGCACACCAGCA
  GCCCAGCTGTACCCTGGACGAATGTTTTCAGTCCTATACCAAGGAGGAACAGCTGGCCCAGGATGATGCA
  TGGAAGTGCCCTCACTGCCAAGTCCTCCAGCAGGGTGTGGTCAAGCTGAGTCTGTGGACTCTGCCCGACA
  TCCTGATCATCCATCTGAAACGGTTCTGTCAGGTGGGAGAGAGAAGGAACAAGCTCTCCACGCTGGTGAA
  GTTCCCACTGTCTGGACTCAACATGGCTCCCCATGTAGCACGGAGGAGCACCAACTCCAAGGCGGGGCCC
  GGCCCTTGGTCCTCCTGGAAGCAGCCGATCTGCCTCCCCACCACCTACCCGCTGGACTTCCTCTACGACT
  TGTATGCTGTCTGCAACCACCATGGCAGTCTGCAAGCCTATTGCCGGAACTCCCTGGACGGCCAGTGGTA
  CAGCTATGACGACAGCACAGTGGAGGCCCTGCGAGAGGATGAGGTCAACACCAGGGGGGCTTACATCCTA
  TTCTACCAGAAGCGGAACAGCATCCCTCCCTGGTCAGCCAGCAGCTCCATGAGAGGTTCCACCAGCTCCT
  CCTTGTCTGATCATTGGCTCATGAGACTCGGGAGCCTCAACAACAGCACAAGGGGAAGCTTGCTGTCCTG
  GAGCTCGGCCCCCTGCCCCTCCATGGCCCGGGTACCCGACTCTCCTGTCTTCACCAATGGTGTCTGCCAC
  CAGGACAAGGGAGGAGTTGAGACCAGACCTTTGGTTCGGGGTGTTGGTGGCCGAAGTATCAGCATGAAGG
  CATCTCCTGCTTCCCGATCCAGGCATGGGCCCTTCAAGACCATGCCCCTGAGGTGGTCATTTGGACACAG
  GGAGAAACGTCCCGGGGCATCCGTTGAACTGGTGGAATACCTAGAGTCCAGAAGAAGACCCCGTTCTACC
  AGCCAGTCCATTGTGCCGCTCTTGACTCGTGCTGCTGGTGGGGAAGAGACTTCAGCGTCACCGAGGTCAG
  ATGGCACCCTTCCTGCTAAAAGTGAAGACAGCGGTCGAGCCATTGGGCAAGGAACAACTGGGGTGCCCCT
  TTCCTCATGCCACCTCAACCACCATCCAGCCCTGGGGTCTTTAGATGACAGTCTGCACACTGCCAGGACA
  CGAACGGGGAATGTAAGTCAGGATATCAGGCTCCCAAAAAAGTTTGACCTGCCCCTCACTGTGATGCCCT
  CAGTGGGGGATGAAAAGCCAGCTCGCCCTGAGGGCCAGAAGATGACACCTTGGAAGGGAAGTAGCCAGGT
  GGGAAGCCAGAGTAGCCCACCTTCCCCCTCCACTGGCTTGCTTAGAAACTTTAAGGACAGTGGCCCAGGT
  ACCTTACCCAAGATGAAGTCTAAGGCTGCCATGGAGGAAAGGGCCCCTGACAAAGACAGGGGACAGGGGA
  CATTCACTCTTTTGAAGTCTGTGTTTTGGAAGAAAGAACACAAGAGGACTGTCAGGACTGAGAGCTCACC
  ACCAGCACCCCCAATCTCCCTAGGGAGTGACCGGCTGAGCCCTGCTGCCATGAATGAGCAGGCCCTCAGG
  ATTCGAGAGAGCCCAGCCAAGGGCCTGGGCAACCACATGGAGAGGGACATCAGATCTGCGCCCAGCTCTC
  TCCACCTCCCCCGAAAAGCCAGCAGGCCCCCTCGAGCCAGTACAGCTGGCACCTCACAAAGGACCATTCC
  TGGGGAACAGATTTCTTATGGCACCCTGCAGAGAGTGAAATACCACACCCTTTCCTTAGGTCGAAAGAAA
  TCCTTACCTGAGTCCAGCTTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001291049
• Insert Size: 3384 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291049.1, NP_001277978.1
• RefSeq Size: 4447 bp
• RefSeq ORF: 3384 bp
• Locus ID: 216835
• UniProt ID: Q8BUM9
• Cytogenetics: 11 B3
• Gene Summary: May recognize and hydrolyze the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate splice site in the central coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.