Slc4a10 (NM_001242379) Mouse Untagged Clone

CAT#: MC229374

Slc4a10 (untagged) - Mouse solute carrier family 4, sodium bicarbonate cotransporter-like, member 10 (Slc4a10), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Slc4a10
• Synonyms: mKIAA4136; NCBE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229374 representing NM_001242379
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGATTAAAGACCAGGGAGCCCAAATGGAGCCGCTGCTGCCTACGAGAAATGATGAAGAAGCCGTTG
  TGGATAGAGGTGGAACACGCTCTATTCTCAAAACACATTTTGAGAAAGAAGATTTAGAAGGTCATCGGAC
  ATTATTTATTGGAGTTCATGTGCCCCTGGGTGGAAGAAAAAGCCATCGTCGTCACAGGCATCGTGGTCAT
  AAGCACAGAAAGAGGGACAGAGAGAGAGATTCGGGACTGGAGGATGGAAGAGAGTCCCCTTCTTTTGACA
  CCCCATCGCAGAGGGTGCAGTTTATTCTTGGAACTGAGGACGATGATGAGGAGCACCTCCCTCATGACCT
  TTTCACAGAGCTGGATGAGATTTGCTGGCGTGAAGGGGAAGATGCTGAGTGGCGAGAGACAGCCAGGTGG
  TTGAAATTTGAAGAGGATGTGGAAGATGGAGGAGAAAGATGGAGTAAGCCCTATGTGGCCACGCTTTCAT
  TACACAGCTTGTTTGAGTTGAGAAGCTGCATCCTGAATGGAACTGTGCTACTGGACATGCATGCCAACAC
  GATAGAAGAAATTGCAGATATGGTCCTTGACCAGCAGGTCAGCTCAGGCCAGCTGAATGAAGATGTTCGC
  CACAGGGTCCACGAAGCATTGATGAAGCAGCATCATCACCAGAATCAGAAAAAACTGGCTAACAGGATTC
  CTATTGTCCGATCTTTTGCTGATATTGGCAAGAAACAATCAGAACCAAATTCCATGGATAAAAATGCAGG
  TCAGGTTGTTTCTCCTCAGTCTGCTCCAGCCTGTGCTGAGAATAAAAATGATGTCAGCAGGGAAAACAGC
  ACTGTAGACTTCAGCAAGGTTGATCTGCATTTTATGAAAAAGATTCCTCCGGGTGCTGAAGCTTCAAACA
  TCTTGGTAGGAGAACTGGAGTTCCTAGACAGAACTGTGGTTGCCTTTGTCAGGTTGTCTCCAGCTGTCTT
  GCTCCAAGGACTTGCTGAAGTTCCAATCCCAAGCAGATTTCTGTTCATCCTTCTGGGACCCCTGGGAAAG
  GGTCAACAGTACCACGAGATTGGCAGATCGATTGCGACCTTAATGACTGATGAGGTGTTTCATGATGTTG
  CTTACAAAGCTAAAGACCGCAATGACTTGGTATCAGGAATTGATGAGTTTCTGGATCAGGTTACCGTTCT
  TCCTCCTGGAGAATGGGATCCAAGCATACGAATAGAACCTCCCAAAAATGTCCCTTCCCAGGAGAAGAGG
  AAGATTCCTGCTGTACCAAATGGAACAGCAGCTCATGGCGAAGCTGAGCCACATGGAGGACACAGCGGAC
  CTGAACTCCAGCGAACTGGGAGGATTTTTGGGGGACTTATATTAGATATCAAAAGAAAGGCTCCATTCTT
  CTGGAGTGACTTCAGGGATGCTTTCAGCCTGCAGTGCTTAGCATCGTTCCTGTTTCTCTACTGTGCATGC
  ATGTCTCCTGTCATCACATTTGGAGGACTGTTGGGAGAAGCAACTGAAGGTCGTATAAGTGCAATCGAAT
  CACTCTTTGGAGCATCTATGACCGGGATAGCCTATTCTCTTTTTGGTGGACAGCCCCTGACCATATTAGG
  CAGCACAGGACCTGTTTTGGTGTTTGAAAAGATCTTGTTTAAGTTTTGCAAGGAATACGGCCTGTCGTAC
  TTGTCCTTACGGGCCAGCATTGGGCTCTGGACTGCAACACTGTGCATCATCCTTGTGGCCACGGACGCGA
  GCTCACTCGTCTGCTACATCACCCGGTTTACCGAAGAGGCTTTTGCTTCTCTCATTTGCATCATTTTTAT
  CTATGAAGCCCTGGAGAAGTTGTTTGAGCTCAGTGAAACCTATCCAATCAATATGCACAATGATTTGGAA
  CTGCTGACACAATACTCATGTAACTGTATGGAGCCACATAGTCCCAGCAATGACACACTGAAGGAATGGC
  GGGAGTCCAACCTTTCTGCCTCTGACATAATCTGGGGGAACCTAACTGTGTCAGAGTGCAGATCACTGCA
  CGGGGAGTATGTCGGGCGAGCCTGTGGCCATGGCCACCCCTACGTGCCAGATGTTCTCTTCTGGTCGGTG
  ATCCTGTTCTTCTCCACAGTTACCATGTCAGCCACCCTGAAGCAGTTCAAGACCAGCCGCTATTTCCCAA
  CCAAGGTTCGATCCATAGTGAGTGATTTTGCGGTTTTTCTTACAATTCTGTGTATGGTTTTAATTGACTA
  TGCCATTGGGATCCCATCACCAAAACTACAAGTACCAAGCGTTTTCAAGCCGACCAGAGACGACCGTGGC
  TGGTTTGTTACACCTTTGGGTCCAAACCCATGGTGGACAATCATAGCTGCCATCATCCCAGCTTTACTCT
  GTACTATTCTGATTTTCATGGACCAGCAGATTACAGCTGTCATCATCAACAGAAAAGAGCACAAGCTAAA
  GAAAGGTTGTGGCTATCACCTGGATCTGTTAATGGTGGCAGTCATGCTCGGGGTCTGCTCCATTATGGGC
  CTGCCATGGTTTGTGGCTGCCACAGTTCTCTCCATCACTCATGTCAACAGCCTCAAGCTCGAATCAGAGT
  GCTCTGCTCCAGGAGAACAACCCAAGTTTCTCGGCATTCGGGAGCAGAGGGTGACCGGGCTCATGATTTT
  TATTCTTATGGGTTCATCCGTTTTCATGACCAGCATTCTGAAGTTTATCCCCATGCCAGTGTTATACGGA
  GTGTTTCTTTATATGGGTGCTTCGTCTCTCAAAGGAATTCAGTTATTTGATAGAATAAAGCTCTTCTGGA
  TGCCAGCCAAACATCAACCAGATTTCATCTATCTAAGGCACGTGCCCCTCCGGAAAGTCCATCTCTTCAC
  AGTCATTCAGATGAGTTGTCTCGGCCTTCTGTGGATAATCAAAGTTTCGAGAGCTGCTATTGTCTTTCCT
  ATGATGGTGTTGGCACTAGTGTTTGTGAGAAAGTTGATGGACTTCTTGTTTACCAAACGGGAACTCAGCT
  GGCTTGATGATTTAATGCCTGAGAGTAAAAAGAAGAAACTTGAAGATGCTGAGAAAGAAGAAGAACAAAG
  TATGCTAGCCATGGAGGACGAGGGCACAGTACAACTCCCACTGGAGGGACACTACAGAGACGACCCGTCT
  GTGATCAATATTTCTGATGAAATGTCAAAGACTGCCATGTGGGGGAACCTTCTAGTTACTGCTGACAACT
  CAAAAGAAAAGGAGTCACGCTTTCCTTCTAAAAGCAATGAAAGCCGAAAGGAGAAGAAAGCTGACTCAGG
  GAAAGGCGTTGACAGGGAGACTTGTCTATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001242379
• Insert Size: 3321 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001242379.1, NP_001229308.1
• RefSeq Size: 5424 bp
• RefSeq ORF: 3321 bp
• Locus ID: 94229
• Cytogenetics: 2 C1.3
• Gene Summary: Sodium/bicarbonate cotransporter which plays an important role in regulating intracellular pH (PubMed:10993873, PubMed:20566632). Has been shown to act as a sodium/bicarbonate cotransporter in exchange for intracellular chloride (PubMed:10993873, PubMed:20566632). Has also been shown to act as a sodium/biocarbonate cotransporter which is not responsible for net efflux of chloride, with the observed chloride efflux being due to chloride self-exchange (By similarity). Controls neuronal pH and may contribute to the secretion of cerebrospinal fluid (PubMed:18165320). Reduces the excitability of CA1 pyramidal neurons and modulates short-term synaptic plasticity (PubMed:26136660). Required in retinal cells to maintain normal pH which is necessary for normal vision (PubMed:23056253). In the kidney, likely to mediate bicarbonate reclamation in the apical membrane of the proximal tubules (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (3) lacks an alternate internal segment compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.