Tcerg1 (NM_001289526) Mouse Untagged Clone

CAT#: MC229367

Tcerg1 (untagged) - Mouse transcription elongation regulator 1 (CA150) (Tcerg1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tcerg1
• Synonyms: 2410022J09Rik; 2900090C16Rik; AI428505; ca150; CA150b; FBP23; FBP28; p144; Taf2s
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229367 representing NM_001289526
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCGTGGCGGGGACGGAGGGGAAGGCGAGCGGTTCAACCCGGGGGAGCTGAGGATGGCTCAAC
  AGCAGGCCTTGAGGTTCCGAGGCCCAGCTCCCCCACCAAATGCCGTGATGCGAGGCCCGCCACCTCTCAT
  GAGACCACCTCCTCCTTTCGGCATGATGAGAGGCCCTCCTCCACCACCCAGGCCCCCCTTTGGACGGCCT
  CCTTTTGATCCTAATATGCCACCAATGCCTCCTCCAGGAGGAATACCTCCACCTATGGGCCCACCTCACC
  TACAGAGACCACCTTTCATGCCTCCACCCATGGGAGCTATGCCTCCTCCTCCGGGTATGATGTTCCCACC
  AGGAATGCCTCCGGGGACTGCTCCTGGTGCTCCAGCACTGCCTCCCACTGAGGAGATATGGGTAGAAAAT
  AAAACTCCAGATGGGAAGGTTTATTACTACAATGCTCGGACTCGTGAATCTGCATGGACCAAGCCAGATG
  GAGTTAAGGTTATTCAGCAATCAGAACTGACGCCCATGCTTGCAGCCCAAGCACAGGTTCAGGCCCAGGC
  CCAGGCCCAGGCCCAAGCACAGGCTCAGGCCCAGGCCCAGGCACAAGCCCAGGCTCAGGCCCAGGCCCAG
  GCCCAGGCTCAGGCACAAGCCCAGGCTCAGGCCCAGGCCCAGGCCCAGGCTCAGGCTCAAGCCCAAGCCC
  AAGCGCAAGCACAAGCCCAGGCCCAAGCCCAGGCCCAGGCTCAGGCCCAGGCACAAGTCCAAGCACAAGC
  AGTAGGCGCTCCCACCCCTACCACCAGTAGCCCAGCACCTGCAGTGTCCACGTCAACTCCGACATCTACC
  CCATCCTCCACCACAGCTACCACGACAACTGCTACTTCAGTTGCACAGACAGTATCAACACCCACAACAC
  AAGACCAGACCCCAAGTTCTGCTGTTTCAGTTGCCACACCTACAGTTAGTGTTTCAGCTCCTGCTCCTAC
  AGCCACACCTGTGCAAACCGTACCCCAGCCGCACCCACAGACGTTACCTCCTGCTGTTCCTCACTCGGTA
  CCTCAGCCGGCAGCAGCAATACCTGCTTTCCCACCAGTAATGGTGCCTCCGTTCCGTGTGCCCCTGCCTG
  GCATGCCAATCCCACTTCCAGGTGTACTACCAGGAATGGCCCCTCCTATAGTACCCATGATCCATCCCCA
  GGTTGCTATTGCAGCTTCACCTGCTACCCTAGCTGGGGCAACAGCAGTTTCTGAGTGGACTGAATATAAA
  ACAGCAGATGGGAAGACGTACTATTATAATAATAGAACACTAGAATCCACGTGGGAGAAGCCCCAAGAAC
  TGAAGGAGAAAGAAAAATTAGATGAGAAGATTAAAGAACCAATTAAGGAGGCCTCTGAAGAGCCATTGCC
  AATGGAGACTGAGGAGGAAGATCCTAAGGAAGAGCCTGTGAAGGAGATAAAGGAGGAGCCCAAAGAAGAA
  GAGATGACTGAAGAAGAAAAGGCTGCCCAGAAGGCAAAGCCAGTAGCTACTACTCCTATTCCTGGTACTC
  CATGGTGTGTTGTTTGGACTGGTGATGAGAGGGTCTTCTTTTACAATCCCACCACCCGCCTTTCTATGTG
  GGACCGGCCTGATGATTTGATTGGAAGGGCGGATGTTGACAAAATTATTCAAGAGCCCCCTCATAAGAAA
  GGATTGGAAGACATGAAGAAGCTAAGGCACCCGGCTCCGACAATGCTGTCCATCCAAAAGTGGCAGTTCT
  CAATGAGCGCAATTAAAGAAGAACAAGAGTTAATGGAAGAAATGAATGAAGATGAGCCTATTAAAGCAAA
  AAAGCGGAAGAGAATGTCAAAGAAGTCCTTTATGTGGATTGCCCGAGCTTCTCTATTTAGGAGAGATGAT
  AATAAAGACATTGATTCAGAGAAAGAAGCTGCCATGGAAGCTGAAATTAAAGCTGCCCGAGAAAGGGCCA
  TTGTCCCTCTGGAGGCTCGAATGAAGCAGTTCAAGGACATGCTGCTAGAGAGAGGGGTGTCTGCTTTTTC
  AACATGGGAGAAAGAGTTGCACAAGATAGTATTTGACCCTCGGTACTTGCTTCTCAATCCTAAAGAAAGA
  AAACAGGTGTTTGATCAGTATGTCAAGACCAGAGCAGAGGAAGAACGCAGGGAAAAGAAAAACAAAATAA
  TGCAAGCCAAGGAAGATTTCAAAAAAATGATGGAAGAAGCAAAGTTTAATCCAAGAGCTACTTTTAGTGA
  ATTTGCTGCCAAACATGCTAAAGATTCAAGATTCAAAGCAATTGAAAAGATGAAAGATAGAGAAGCATTG
  TTTAATGAGTTTGTGGCAGCTGCAAGGAAGAAAGAAAAAGAAGATTCGAAGACGAGAGGGGAGAAGATTA
  AATCAGATTTCTTTGAACTGTTATCTAATCATCACTTGGATAGTCAATCTCGATGGAGCAAAGTAAAAGA
  CAAAGTAGAAAGTGACCCCCGATATAAAGCGGTGGATAGTTCATCAATGAGAGAAGACCTTTTCAAACAG
  TACATCGAGAAAATAGCCAAGAATTTAGACTCAGAAAAAGAAAAAGAGCTGGAGAGGCAAGCCCGCATTG
  AGGCAAGCCTTCGAGAACGGGAAAGAGAAGTTCAGAAGGCGCGCTCTGAGCAGACGAAAGAGATCGATCG
  GGAGAGAGAGCAGCACAAACGGGAGGAGGCTATCCAGAACTTCAAGGCTCTTCTGTCTGACATGGTACGT
  TCTTCAGATGTGTCCTGGTCTGATACTAGGAGGACCCTCCGGAAAGACCACCGCTGGGAATCTGGGTCCT
  TATTGGAAAGAGAGGAGAAAGAGAAACTTTTTAATGAACATATTGAAGCGCTTACCAAAAAGAAAAGGGA
  GCACTTTAGGCAGCTTCTGGACGAAACCTCTGCGATTACCTTAACATCCACGTGGAAAGAAGTGAAAAAA
  ATAATTAAGGAAGATCCTCGGTGCATTAAGTTCTCCTCCAGTGACAGGAAAAAACAAAGGGAGTTTGAAG
  AATACATCAGAGACAAATACATCACTGCCAAAGCTGACTTCAGGACACTTTTGAAAGAGACCAAATTCAT
  TACATACAGATCCAAAAAGTTAATCCAAGAATCTGATCAGCACCTGAAAGATGTAGAAAAGATTTTGCAG
  AATGACAAACGGTATCTAGTACTGGACTGTGTCCCCGAGGAGAGGAGGAAACTAATTGTGGCCTATGTTG
  ATGACCTGGATCGCCGAGGTCCACCCCCACCTCCCACAGCATCAGAGCCCACTAGACGATCAACAAAATA
  A
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289526
• Insert Size: 3291 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289526.1, NP_001276455.1
• RefSeq Size: 4397 bp
• RefSeq ORF: 3291 bp
• Locus ID: 56070
• UniProt ID: Q8CGF7
• Cytogenetics: 18 B3
• Gene Summary: Transcription factor that binds RNA polymerase II and inhibits the elongation of transcripts from target promoters. Regulates transcription elongation in a TATA box-dependent manner (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region and contains an alternate 3' exon, compared to variant 1. This results in a shorter isoform (2), compared to isoform 1.