Radil (NM_001289588) Mouse Untagged Clone

CAT#: MC229365

Radil (untagged) - Mouse Ras association and DIL domains (Radil), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Radil
• Synonyms: AI536456; D930005D10Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229365 representing NM_001289588
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTTATGGGACTCAGTTGATCATGTCTCCTCCCACCAAGAATAAGCTGAAGCGACAGAGCCAGCTCC
  TATCCACAATGCTGTCCCGGACACTGAGCTACAAGTATCGTGACCTGGATTCCACCTTCTGCAGCCTAGG
  TGCCAGTGACGACCCCTCAGAGCTCTCCACGCAGCTCTCGGCCCCCGGGGTCCTCAAGGTGTTTGGGGAC
  AGTGTCTGTACAGGCACCCACTATAAGAGTGTCTTGGCCACTGGCTCCTCCAGTGCCCAGGAGCTGGTGA
  AAGAGGCACTGGAGCGCTATGCCCTGGACCCTGAGTGTGCTGGCCAGTACGTGCTATGTGATGTTGTGGG
  CCAAGCTGGGGACTCTGGGCAGCGGTGGCAGGCCCAGTGCTTCCGAGTATTTGGCGACAATGAGAAGCCC
  CTCTTGATCCAGGAGTTATGGAAACCCCGAGAAGGCTTGTCCCGGAGGTTTGAGCTAAGGAAGAAGTCAG
  ATGTGGAAGAGCTTGCATCGAGGGATGTGGATACCACCACCGCAGGGATAAACGCCCAAGCCCGGAGGCT
  ACAGCGTATCCGTGCCAAGGGAACCCCAGCCCTCACCTCTGAAGCTGCTCAAAGCTCCCCACCGACCAGG
  CTGCGCCGTACAGTCAGCGAGACCAGCCTAAGCCCAGCACCATCGCTACCTGAGGCTGCTCAGAGGCCTG
  AGGAGCCCGTCCCTGAGGCCATGCGTTACTCGCTGTACCAGTGCCCGCACCTGCTGCTGTTGCAGGGCTA
  CAGCCAGCAGCACGACAGCCTGGTGTACGTGCTCAGCAAGGAACGGCACACAGTGGGCCAGCGCACACCG
  TCTAGCAAACCCAGCATCAGCTTGTCTGCCCCAGACATCCTGCCCCTGCACTGTACCATTCGCCGCCATC
  AGTCCCCAGAGGGTGGCCCGGCAGGGACCAGGCTAGTGCTGGAGCCCATCACAGGGGCTTCGGTGTCAGT
  CAACTTCTCAGAAGTGGGAAGGAACCCTGTGGTGCTACAACATGGCGACCTGCTCTCCCTGGGTCTCTAT
  TACCTGCTGCTGTTCAAGGACCCTGGGCAGGCACAGCCACTGCCTGCTTGTGCCCTCGCCCGTCTTGGGG
  CGGCACCACAGAGCTGTAGAATGTGTGGTGCGGTGCTCAGAGCCCGTGGGGCCCCTTCCTTGCCTGCTGC
  TGTGGTCCGGCGGCGGTCACTGCTCCTGGAGTTTGAGCCCGATGTGGAGGACACACTGCTGCAGAGGATC
  ATGACACTGATTGAGCCTGGGGGTGACGACCACAAGCTGACACCTGCCTTCCTCCTCTGCCTCTGCATCC
  AGCACTCCGCCATGCACTTCCAGCCGGGTACCTTCAGGCATCTCCTGCTCAAGATCTCCAAAAGGGTCCG
  AGACACTGTCTGGGAGAAGACCAAAGAACTAGCGGAAAAGCAGGCACAACTCCAGGAGCCCATCTCCTGG
  GCCAGCTTCCCCATGGCCGACCTGGTTCCCGACCTGCAGCACATCCTGTTTTGGATGTCAAACTCCATCG
  AGCTCCTGTACTTCATCCAGCAGAAGTCCCCGCTGTACGTGCAGAGCATGGAGGAGGAGCTGGACGTCAC
  AGGCTCCAAGGAGTCGCTGTTCTCCTGCACACTGACGGCCAGCGAGGAGGCCATGGCTGCCCTGGAGGAG
  GTGGTGCTCTATGCTTTCCAGCAGTGCGTGTATTACCTCTCCAAGTGCTTGTATGTCTGCCTCCCGGCCC
  TCTTGGAATGCCCCCCATTTCAGACAGAGCGCAGGGAGAGCTGGCGCTCAGGTCCAGCTCTGCCCGAGGA
  GCTTCGGCGAGTGGTGTCAGTGTTCCAGGCCACCTTGGACCTCCTGCAGCAGCTGCAGATGCATCCTGAG
  GTGGCCTCCCAGATGCTGGCTTATCTCTTCTTCTTCTCTGGCACACTGCTCCTCAACCAGGTCCTAGACA
  AGGGGCCCTCTCTGAGTTGCTTCCATTGGCCCAGGGGTGTGCAGGTGTGTGCCCGCCTGCAGCAGTTCCT
  GGAGTGGGCCCGGAGTGCTGGCCTTGGAGCACCTGCTGAGCGCTTCTTCCGGAAGCTTTCCTGCACCCTG
  CATCTGCTGGCCACTCCCAGGGCTCAGCTCATCCAGATGAGCTGGGCCACCCTGAGGGTCACATTCCCTG
  CCCTGAACCCTGCTCAGCTGCACCGGCTTCTGACTCAGTACCAGCTGGCCTCTGCCATGGGCCCTGTGAG
  CGCTTGGGAGCCAGGAGCCCCAGACGGCCCGGAGGCCTTCCAGTCAGAGGACATCCTGGAGTCCTATGAA
  AACCCGCCGCCCATTGTTCTGCCGAGCCAGGGCTTCCAGGTAGACCTGGAAGCCGACTGCGTGGAGGACA
  GCATCTACCAGCACCTGCTCTACATCCGCCACTTCCTGTGGGGGCTGCGGGGCCAAGCCAGCCCTGACAG
  TGGGCCTGCCCAGCCCGAGAGCATCGAGGGAGCCATCATGGATACCTGGGCCATCCCCTCCTCTGGGCCT
  CAACGTCTGTCCACCTTCTCTTCCCAGGGCCTGTACCACACAATTCCTGAGGGCCATCTGGAAGGCCATG
  GCTGCCCCCTGGCCAATAGGGACCCAGGCAGAGTTGCTGTTGAAACTGCCCCTCCCCATTCTCTTCCTGT
  CACGGGAGCTCCCCGGGCTCAGGGTCCCCCTGGAAGACAGCCTACCCGAGGGGACCGCAGGGGCTCCCAG
  GCTGGCTCTCTGCACACTGACTCCTCCTGCATGCTCACTCCGCCCAGCACGCCACTGGGCCTGGAGCCTG
  CAGGCCCCAGCTGGCCAGAGCCCAGCGGCCTCTGTGGAAGAGCAGTCCTTGACGGGCAGAGGAATGGGCC
  AGGTGGTCTGCCTGGTGCAGTCCTAGAAGGAGATGCGATTCAGGATGCTGAGCCTCCTGCTGAGGCCTCC
  AGCCCCAGCTCCAGCGCTGAGGACTTCTGCTACGTGTTCATGGTGGAGCTGGAGCGAGGCCCCTCGGGGC
  TGGGAATGGGCCTGATTGATGGGATGCACACACCTCTGGGGGCCCAGGGACTCTACATACAGACCCTGCT
  TCCCGGGAGCCCCGCGGCATCTGATGGGCGGCTGTCACTCGGAGACCAAATCCTGGAGGTGAATGGCAGC
  AGCCTGAGGGGTGTCAGCTACATGAGGGCAGTGGATTTGATCCGACATGGAGGCAAGAAGATGCGATTTT
  TAGTTGCCAAGTCTGACATGGAAACGGCCAAGAAAATCCGCTTCCGCAATCCCCCTTCCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289588
• Insert Size: 3282 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289588.1, NP_001276517.1
• RefSeq Size: 3796 bp
• RefSeq ORF: 3282 bp
• Locus ID: 231858
• Cytogenetics: 5 G2
• Gene Summary: Downstream effector of Rap required for cell adhesion and migration of neural crest precursors during development.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes isoform 1.