Sec24a (NM_001290785) Mouse Untagged Clone

CAT#: MC229358

Sec24a (untagged) - Mouse Sec24 related gene family, member A (S. cerevisiae) (Sec24a), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sec24a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229358 representing NM_001290785
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCAGCCCCGGATCCCCGCTGCCCGCGGTGCCGCAGCTAGCCTCCAGGCCCAGAACGGAGCCGCCT
  CAGCCTCGGGGTCTCCCTACACCAACGGTCCTGTCCACAATACACTGATGTCTCCACAAGTGTCATCGAG
  TCAAGGCTATGATTCGCAACCTCCAGGATCCTACCCTCGTCCAATGCCAGCAAAGACTTTGAATCCATTC
  TCTGCACAGTCGAACTACGGTGGTTCTCAGGGATCTGGGCAGACTCTTAATAGTCCACTTGTGACTTCCG
  GTCCAGTACTACCTTCACTTCATAGTGGTCCTGTTCCCCGAATGCCACTACCTACTTCTCAGAACCCAGC
  TGCTACACCAATGCCTTCTGGTAGCTTTCTTCCTGGAGCCAACCCACCCCCACCTTTGAATTGGCAATAT
  AACTATCCATCCACAGGACCGCAGACTAACCATTTTCCTCATGTAGCTCCACCAACTCTACCTGGGAATC
  CAAATTTAACAGCGGATCATCAATATGTTTCTTCTGGAGACCCTGCACTTCAAACCAGCTTCAAAAAGCC
  AGGTTCTGCACTTCCCTTACAGAACCCACCTCTGCCCCCCACTTTTCAGCCAGGAGCTCCTCCTGGGCCC
  CCTCCAGCTGGAGGCCCACCTCCCAGCCGGGGCCCCGCGCCCCAGAAAACACCTCCTCGGGCTGCACCCC
  CACCTTCATTTAACTCAGCTGTCAATCAGGAAGGTATTACGTCAAATGCCAATAATGGATCTACGGCGGC
  TCATAATACTTATGATGAGATTGAAGGAGGTGGCTTCTTGGCAACACCACAGCTTGTTAATCAGAACCCC
  AAAACAAGCCGAAGTGTGGGCTCTGCATACCCCTCCCTGCCACCAGGCTACCAGAACAGCGCACCACCTG
  TGGCTGGGATGCCACCACCCTCCCTCAGTTACCCAAGTGGCCCCCAGGCCTTTACTCAGACTCCCTTAGG
  TGCTAATCATTTAACTGCAAGCATGAGTGGATTAAGTCTGCATCCAGAGGGACTAAGAGTTGTCAATCTT
  CTTCAAGAAAGGAACATGCTTCCTTCCACACCACTGCAGCCTCCAGTCCCAAATCTGCTTGAAGACATCC
  AGAAACTCAACTGTAACCCAGAGTTGTTCCGATGCACGCTGACGAGTGTCCCTCAGACTCAGGCCTTACT
  GAATAAAGCCAAGCTTCCTCTGGGGCTGCTGCTCCACCCCTTCAAGGACCTAGTGTTGCCCGTGGTCACC
  TCCAGCACAATTGTGAGATGCCGTTCATGCAGGACTTACATTAACCCTTTCGTCAACTTCCTTGATCAAA
  GAAGGTGGAAATGTAACTTATGTTATCGAGTCAATGATGTTCCTGAAGAATTCATGTACAACCCTTTAAC
  CAGAGTTTATGGAGAACCTCACAAAAGACCTGAAGTCCAGAATGCTACTATTGAGTTTATGGCTCCTTCA
  GAATACATGTTACGACCACCACAACCTCCAGTGTACCTCTTTGTATTTGATGTATCTCACAACGCAATAG
  AAACTGGATACTTGAATTCAGTTTGCCAGAGTTTGTTAGACAATCTGGATCTGCTTCCTGGCAACACTAG
  GACAAAAATCGGTTTCATAACATTTGATAGCACTATCCATTTCTACAGTCTTCAGGAAGGTCTCTCCCAG
  CCTCAGATGCTAATAGTTTCAGATATTGACGATGTTTTTATCCCTATGCCAGAGAACTTACTAGTAAACT
  TAAATGAAAGTAAAGAGCTTGTTCAAGATTTACTGAAAACGCTGCCACAAATGTTTACGAAGACGCTGGA
  GACCCAGAGTGCCTTGGGTCCTGCACTTCAGGCTGCATTCAAGCTGATATCACCAACTGGTGGTCGAATG
  TCTGTCTTCCAAACACAACTCCCAACGCTTGGTGTAGGAGCTCTGAAACCACGAGAGGAACCCAACCAAA
  GGTCATCTGCTAAGGAAATACATCTGACACCATCCACTGACTTCTATAAGAAATTAGCCTTGGACTGTTC
  TGGGCAGCAAGCAGCTGTTGACTTATTCCTTCTCAGTGGACAGTATTCTGATTTGGCTTCCCTAGGCTGC
  ATCTCTCGGTACTCAGCGGGTAGTGTGTATTACTATCCCTCGTACCATCACCAGCACAACCCAGTGCAAG
  TACAGAAACTACAGAAGGAACTACACAGGTACCTCACTCGGAAGATTGGATTTGAGGCAGTCATGAGGAT
  CCGGTGCACCAAAGGTCTTTCCATTCATACTTTCCATGGGAACTTCTTCGTCCGTTCTACAGACTTACTG
  TCTCTTCCCAATGTCAACCCAGATGCTGGGTATGCGGTCCAGATGTCAGTAGAAGAGAGTCTCACTGACA
  CTCAGCTGGTTTCCTTCCAGTCAGCACTCTTATATACATCAAGCAAAGGTGAAAGAAGAATCCGTGTGCA
  TACTTTGTGTTTGCCGGTAGTTTCTACCCTGAATGAAGTCTTTCTTGGAGCTGATGTGCAAGCAATTTCA
  GGGTTATTAGCCAATATGGCTGTTGACAGGTCGGTGACTGCCAGCCTGAGTGATGCCCGGGATGCTCTGG
  TGAACGCTGTCATCGACTCTCTCTCAGCTTACCGCTCTTCAGTCCTGAGTGGTCAGCAGCCAGGCCTCAT
  GGTTCCTTTCTCCTTGAGGCTTTTCCCACTTTTTGTGTTGGCTCTCCTAAAACAGAAATCTTTCCAGACT
  GGGACAAGTATACGTCTAGATGAACGAATTTTTGCCATGTGTCAAGTGAAAAGCCAGCCATTGGTTCACC
  TCATGCTCACAACACATCCCAGTTTGTACAGAGTTGACAACCTCTCAGATGAGGGAGCTCTCAACATCAA
  TGACAGAACCATACCTCAGCCCCCCATTCTTCAGCTCTCGGTGGAGAAGTTGAGCAGAGATGGAGCGTTC
  CTCATGGATGCAGGCTCATTACTGATGCTTTGGGTTGGAAGAAATTGTTCACAGAATTTTCTCAGCCAAG
  TTCTGGGAGTTCAAAACTATGCATCAATTCCACAGACAATGACAGATCTTCCAGAACTTGATACACCAGA
  ATCTGCCAGAATAGCAGCGTTCATCTCATGGCTCAGAGAACAGAGGCCATTTTTCCCAGTACTCTATGTA
  ATCAGGGAGGAGAGTCTTATGAAAGCAGCCTTCCTTCAGAGCTTGGTAGAAGACAGAACAGAGTCTGCAC
  TGTCATATTATGAATTCCTCCTGCACATACAGCAGCAAGTGAACAAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290785
• Insert Size: 3270 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290785.1, NP_001277714.1
• RefSeq Size: 6795 bp
• RefSeq ORF: 3270 bp
• Locus ID: 77371
• UniProt ID: Q3U2P1
• Cytogenetics: 11 B1.3
• Gene Summary: Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules for their transport to the Golgi complex. Plays a central role in cargo selection within the COPII complex and together with SEC24B may have a different specificity compared to SEC24C and SEC24D. May package preferentially cargos with cytoplasmic DxE or LxxLE motifs and may also recognize conformational epitopes.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.