Clasp2 (NM_001286601) Mouse Untagged Clone

CAT#: MC229356

Clasp2 (untagged) - Mouse CLIP associating protein 2 (Clasp2), transcript variant 6

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Clasp2
• Synonyms: 1500004F14Rik; 8030404L10Rik; C77448; CLASP2beta; mKIAA0627
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229356 representing NM_001286601
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACCTCTGGATGTGCAGCAATCAGATTTATTATTCGGCATACCCATGTACCTAGACTTATTCCTT
  TAATAACAAGCAACTGCACATCAAAGTCAGTTCCTGTAAGGAGACGTTCATTTGAATTTTTAGATTTGCT
  GCTGCAAGAATGGCAGACTCATTCACTGGAAAGACATGCAGCTGTTTTGGTTGAAACGATTAAGAAGGGC
  ATTCATGATGCTGATGCTGAGGCCAGAGTGGAGGCAAGGAAGACATACATGGGCCTTAGGAACCACTTTC
  CTGGTGAAGCTGAAACATTGTACAACTCCCTTGAGCCATCATATCAGAAAAGTCTTCAAACTTACTTAAA
  GAGTTCTGGAAGTGTAGCTTCTCTTCCGCAGTCAGACAGGTCCTCATCCAGCTCACAAGAAAGTCTCAAT
  CGTCCTTTTTCTTCTAAATGGTCAACAGCAAATCCTTCAACTGTAGCTGGAAGAGTATCTGTGGGAGGCA
  GCAAAGCCAACCCCCTTCCAGGAAGCCTGCAGCGTTCTCGAAGTGACATTGATGTGAATGCGGCAGCTGG
  TGCCAAGGCTCATCATGCTGCTGGGCAGGCGGTGCGAAGTGGGCGCTTAGGTGCAGGTGCCCTGAACCCA
  GGCTCCTATGCATCACTAGAGGATACTTCTGACAAGATGGATGGAACAGCATCTGATGATGGTCGGGTGA
  GAGCCAAGCTTTCTACACCGCTTGTTGCTGTGGGAAATGCCAAGACCGACTCTAGAGGGAGAAGCCGGAC
  AAAAATGGTGTCTCAGTCCCAGCCTGGCAGCCGATCTGGGTCTCCAGGAAGAGTTCTAACCACAACAGCC
  CTCTCTACTGTGAGCTCTGGTGCTCAACGAGTCCTGGTCAATTCAGCTTCAGCACAGAAGAGAAGCAAGA
  TCCCAAGGAGCCAGGGCTGCAGCAGAGAGGCCAGCCCATCTCGGCTCTCAGTGGCCCGGAGCAGCCGTAT
  TCCTCGGCCGAGTGTGAGTCAAGGCTGTAGCCGGGAAGCCAGCAGAGAGAGCAGCAGGGACACGAGTCCG
  GTGCGCTCCTTCCAGCCGCTGGGTCCGGGATATGGGATCAGCCAGTCCAGCCGGTTGTCGTCCTCTGTCA
  GTGCCATGCGAGTCCTAAACACAGGCTCCGATGTGGAGGAGGCAGTAGCTGATGCCCTGCTCTTAGGAGA
  CATACGGACTAAGAAAAAACCTGCTCGAAGAAGGTATGAATCATATGGAATGCACTCAGATGATGATGCC
  AACAGCGATGCCTCTAGTGCGTGTTCAGAACGCTCCTATAGCTCTCGAAATGGTAGTATTCCTACCTACA
  TGAGACAGACAGAAGACGTGGCAGAAGTCCTCAACAGATGTGCTAGCTCCAATTGGTCAGAGAGGAAAGA
  AGGCCTCTTGGGTCTGCAGAACTTGTTAAAAAACCAGAGAACGCTAAGTCGAGTTGAACTGAAAAGATTA
  TGTGAAATTTTCACAAGAATGTTTGCAGATCCTCATGGCAAGAGAGTGTTCAGCATGTTCTTGGAGACTC
  TAGTAGATTTCATACAAGTCCACAAAGATGATCTTCAAGATTGGTTGTTTGTTCTGCTGACACAGCTGCT
  GAAAAAAATGGGTGCTGATTTGCTTGGCTCTGTTCAGGCAAAAGTTCAGAAAGCCCTTGATATTACAAGA
  GAGTCTTTTCCAAATGATCTTCAGTTTAATATCCTAATGAGATTTACAGTTGACCAGACCCAAACGCCAA
  GCTTGAAGGTAAAGGTGGCTATCCTTAAGTACATAGAAACTCTGGCAAAGCAGATGGACCCAAGAGATTT
  TACAAATTCCAGTGAAACTCGCCTGGCAGTGTCTCGGGTCATCACTTGGACGACAGAGCCCAAAAGCTCT
  GATGTTCGGAAGGCAGCGCAGTCAGTGCTGATTTCTTTATTTGAACTCAATACCCCAGAGTTTACAATGT
  TACTAGGAGCTTTACCAAAAACTTTCCAGGATGGTGCTACTAAACTTCTTCACAATCACCTCCGGAACAC
  TGGCAATGGCACCCAGAGTTCCATGGGGAGTCCTTTGACGAGACCAACACCTCGGTCACCAGCCAACTGG
  TCCAGTCCTCTTACTTCTCCTACCAACACGTCTCAGAATACGTTATCTCCAAGTGCATTTGATTACGATA
  CAGAGAACATGAATTCTGAAGACATTTATAGCTCCCTTAGAGGCGTCACTGAGGCAATCCAGAATTTCAG
  CTTCAGAAGCCAAGAAGATATGAGTGAGCCAGTGAGGAGGGACCCTAAAAAGGAGGATGGTGACACAATA
  TGTAGTGGTCCTGGGATGTCAGATCCAAGAGCAGGAGGTGATGCTGCTGACGGCAGCCAGCCAGCTCTGG
  ATAATAAAGCATCGTTGCTCCACTCAATGCCACTCCACTCCTCTCCACGCTCCCGTGACTATAACCCATA
  TAACTACTCAGATAGCATCAGTCCTTTCAACAAGTCTGCCCTCAAGGAAGCCATGTTTGATGATGACGCC
  GACCAATTTCCTGATGATCTTTCCTTAGACCATTCTGACCTAGTTGCAGAGTTGTTGAAGGAGCTGTCTA
  ATCATAATGAACGTATAGAAGAAAGAAAAATTGCCCTGTATGAACTCATGAAGCTAACCCAGGAAGAATC
  TTTCAGTGTTTGGGATGAACACTTCAAAACAATATTATTGTTATTGCTTGAGACCCTTGGGGATAAAGAG
  CCTACAATCCGGGCCTTGGCATTAAAAGTTTTAAAAGAAATCTTAAGGCATCAACCAGCAAGATTCAAAA
  ACTATGCAGAACTAACTGTCATGAAAACACTGGAAGCACATAAAGATCCTCACAAAGAGGTGGTGAGATC
  TGCTGAGGAAGCTGCCTCCGTATTGGCTACTTCCATTAGTCCAGAGCAGTGCATCAAAGTGCTTTGTCCC
  ATCATACAAACCGCTGACTACCCTATTAATCTGGCTGCAATCAAAATGCAAACAAAAGTGATAGAGAGAG
  TATCCAAGGAGACCCTTAACATGCTCTTACCAGAGATCATGCCGGGTCTAATACAGGGTTATGATAATTC
  AGAAAGCAGTGTCCGGAAAGCTTGTGTCTTCTGCCTGGTGGCTGTCCATGCAGTGATTGGTGATGAACTA
  AAGCCACATCTCAGTCAACTCACTGGTAGTAAAATGAAGCTGCTGAACCTTTACATCAAGCGTGCACAGA
  CGGGCTCTGCAGGCGCGGACCCCACTGCTGATGTTTCTGGACAGAGTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001286601
• Insert Size: 3270 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286601.1, NP_001273530.1
• RefSeq Size: 5644 bp
• RefSeq ORF: 3270 bp
• Locus ID: 76499
• UniProt ID: Q8BRT1
• Cytogenetics: 9 F3
• Gene Summary: Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (6) differs in the 5' UTR, lacks a portion of the 5' and 3' coding region, and initiates translation at an alternate start codon, compared to variant 3. It encodes isoform f, which is shorter and has a distinct N-terminus, compared to isoform c. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.