Slc12a4 (NM_001253804) Mouse Untagged Clone

CAT#: MC229352

Slc12a4 (untagged) - Mouse solute carrier family 12, member 4 (Slc12a4), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Slc12a4
• Synonyms: AW546649; KCC1; RBCKCC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229352 representing NM_001253804
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTCACTTCACCGTGGTGCCGGTGGACGGGCCGCGACGCGGCGACTATGACAACCTCGAGGGGCTCA
  GTTGGGTGGACTACGGCGAGCGCGCCGAGCGGGAAGACTCGGACGGTGAGGGACAGGGTAACCACAGAGA
  GAATAGCCCTTTCCTTTGCCCTTTGGACGCCTCCAGAGGAAATGACTACTATGACCGGAACTTGGCACTG
  TTTGAGGAGGAGCTGGACATCCGCCCAAAGGTGTCATCTCTCCTGGGCAAGCTTGTCAGCTATACCAACC
  TCACCCAAGGAGCCAAGGAGCACGAGGAGGCTGAGAGTGGAGAAGGTGGCCGACGGAGAGCTGCCAAGGC
  TCCCAGCATGGGCACACTCATGGGAGTGTACCTGCCCTGCCTGCAGAATATCTTTGGGGTCATCCTCTTC
  CTGCGGCTGACCTGGATGGTGGGCACAGCTGGTGTGCTGCAGGCTCTCCTCATTGTCCTCATCTGCTGCT
  GCTGTACCCTGCTGACAGCCATCTCCATGAGCGCCATTGCCACCAACGGCGTGGTTCCAGCTGGTGGCTC
  GTACTTCATGATTTCCCGCTCTTTGGGACCAGAATTCGGAGGTGCTGTGGGCCTGTGCTTCTACCTGGGG
  ACGACATTTGCAGCAGCCATGTATATCCTAGGGGCCATTGAGATCTTGCTGACCTACATTGCTCCGCCAG
  CTGCCATCTTTTACCCATCGGGCACCCATGACATGTCAAGCGCCACCTTGAATAACATGAGAGTGTATGG
  GACCATTTTCCTGACTCTGATGACCCTAGTGGTGTTTGTCGGTGTCAAGTACGTGAACAAGTTTGCCTCG
  CTCTTCCTGGCCTGCGTGATCATCTCCATCCTTTCCATTTACGCGGGAGGCATCAAGTCCATTTTTGACC
  CTCCCGTGTTTCCGGTGTGTATGCTGGGCAATAGGACCCTGTCTCGGGACCAGTTTGACATCTGTGCCAA
  GACAGTCGTGGTGGACAATGAGACAGTGGCCACCCGGCTGTGGACTTTCTTCTGCCACAGCCCCAACCTT
  ACTGCTGACTCCTGTGACCCCTACTTCCTGCTCAACAATGTGACAGAGATTCCTGGCATACCTGGGGCAG
  CTGCTGGTGTGCTCCAGGAGAACCTCTGGAGCGCTTACCTGGAGAAGGGGGAGGTCGTGGAGAAGCATGG
  GCTGCCGTCCACAGACACCCTCGGCCTAAAGGAGAGCCTGTCCCTGTATGTGGTGGCTGACATCGCCACG
  TCCTTCACCGTGCTGGTTGGCATCTTTTTCCCTTCTGTGACAGGCATCATGGCTGGTTCAAACCGCTCCG
  GGGATCTCCGTGACGCCCAGAAGTCTATCCCAGTGGGGACCATTCTGGCCATTGTTACCACTTCGCTGGT
  GTACTTCAGCAGTGTGATCCTCTTCGGTGCCTGCATCGAGGGTGTGGTACTCCGGGACAAGTACGGTGAT
  GGCGTCAGCAGGAACCTGGTGGTGGGCACCCTGGCCTGGCCTTCGCCTTGGGTCATCGTGGTTGGCTCCT
  TCTTCTCAACATGTGGGGCTGGTCTCCAGAGCCTCACTGGGGCGCCACGTTTACTGCAAGCCATTGCCAA
  GGATAACATCATCCCCTTCCTCCGGGTGTTTGGCCATGGGAAAGCAAACGGTGAACCGACGTGGGCCCTC
  CTCCTGACAGCGCTCATCGCTGAGCTGGGCATCCTGATTGCTTCCCTTGACATGGTGGCCCCCATTCTGT
  CCATGTTCTTTCTGATGTGTTACCTCTTTGTAAACTTGGCCTGTGCCGTGCAGACACTTCTGAGGACCCC
  CAACTGGCGGCCCCGGTTCAAGTACTATCACTGGACATTGTCTTTCCTAGGCATGAGTCTCTGCCTGGCT
  CTTATGTTTGTCTCCTCCTGGTACTATGCCCTAGTGGCCATGCTCATCGCAGGCATGATCTACAAGTACA
  TCGAGTACCAAGGGGCCGAGAAGGAGTGGGGCGATGGGATCCGAGGCCTGTCCCTGAGTGCGGCACGCTA
  TGCACTGCTGAGGCTGGAGGAAGGGCCTCCTCACACCAAGAACTGGCGGCCTCAGCTCCTGGTGCTGCTT
  AAGTTAGACGAAGATCTTCATGTGAAGTACCCCCGGCTCCTCACCTTCGCCTCCCAGCTGAAGGCCGGGA
  AGGGCCTGACAATCGTTGGCTCTGTCATCCAGGGCAGCTTTCTGGAGAGCTATGGTGAAGCCCAGGCCGC
  TGAGCAGACCATCAAGAACATGATGGACATTGAGAAAGTCAAAGGCTTCTGCCAGGTAGTGGTGGCCAGC
  AAGGTTCGAGAGGGACTGGCCCACCTCATCCAGTCTTGCGGCCTGGGCGGCATGAGACACAACTCCGTGG
  TGCTGGGCTGGCCCTATGGCTGGCGACAGAGTGAGGACCCACGTGCCTGGAAGACCTTTATTGACACTGT
  GCGCTGCACCACAGCTGCCCACCTGGCCCTGCTGGTGCCAAAGAACATAGCTTTCTACCCCAGCAACCAC
  GAGCGCTACCTGGATGGCCATATCGACGTGTGGTGGATCGTGCATGATGGAGGCATGCTTATGCTGCTGC
  CCTTTCTGCTGCGCCAGCATAAGGTTTGGAAGAAGTGCCGGATGCGCATTTTCACCGTGGCCCAGATGGA
  TGACAACAGCATCCAGATGAAGAAGGATCTGGCCATCTTCCTGTATCACCTCCGCCTGGAAGCTGAAGTG
  GAGGTGGTAGAGATGCACAACAGTGACATCTCTGCCTATACCTATGAGCGGACACTGATGATGGAGCAGC
  GGTCTCAGATGCTGCGGCAGATGAGGTTGACCAAAACTGAGCGGGATCGAGAGGCCCAGCTGGTAAAGGA
  CAGGCACTCGGCTCTTAGGCTGGAGAGCCTCTACTCCGACGAGGAGGAAGAGTCTGTGGCAGGGGCCGAC
  AAGATCCAGATGACGTGGACCAGAGACAAGTACATGGCTGAGCCCTGGGACCCCAGCCACGCCCCTGACA
  ACTTCCGGGAGCTGGTGCACATTAAGCCGGACCAGTCCAACGTGCGGCGCATGCACACTGCTGTGAAGCT
  CAACGAGGTCATTGTCACGCGCTCCCACGATGCCCGTCTGGTTTTGCTGAACATGCCTGGCCCCCCTAAG
  AACAGCGAGGGCGACGAGAACTACATGGAATTCCTTGAAGTCCTAACCGAGGGCCTTGAACGGGTGTTGT
  TGGTGCGTGGCGGCGGCCGGGAAGTCATCACCATCTATTCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001253804
• Insert Size: 3264 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001253804.1, NP_001240733.1
• RefSeq Size: 3832 bp
• RefSeq ORF: 3264 bp
• Locus ID: 20498
• Cytogenetics: 8 53.06 cM
• Gene Summary: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells. May be involved in the regulation of basolateral Cl(-) exit in NaCl absorbing epithelia.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and it encodes the longest protein (isoform 1).