Map4 (NM_001205332) Mouse Untagged Clone

CAT#: MC229351

Map4 (untagged) - Mouse microtubule-associated protein 4 (Map4), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Map4
• Synonyms: AA407148; MAP-4; Mtap-4; Mtap4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229351 representing NM_001205332
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGACCTCAGTCTTGTGGATGCGTTGACAGAACCACCTCCAGAAATTGAGGGAGAAATAAAGCGAG
  ACTTCATGGCTGCGCTGGAGGCAGAGCCCTATGATGACATCGTGGGAGAAACTGTGGAGAAAACTGAGTT
  TATTCCTCTCCTGGATGGTGATGAGAAAACCGGGAACTCAGAGTCCAAAAAGAAACCCTGCTTAGACACT
  AGCCAGGTTGAAGGTATCCCATCTTCTAAACCAACACTCCTAGCCAATGGTGATCATGGAATGGAGGGGA
  ATAACACTGCAGGGTCTCCAACTGACTTCCTTGAAGAGAGAGTGGACTATCCGGATTATCAGAGCAGCCA
  GAACTGGCCAGAAGATGCAAGCTTTTGTTTCCAGCCTCAGCAAGTGTTAGATACTGACCAGGCTGAGCCC
  TTTAACGAGCACCGTGATGATGGTTTGGCAGATCTGCTCTTTGTCTCCAGTGGACCCACGAACGCTTCTG
  CATTTACAGAGCGAGACAATCCTTCAGAAGACAGTTACGGTATGCTTCCCTGTGACTCATTTGCTTCCAC
  GGCTGTTGTATCTCAGGAGTGGTCTGTGGGAGCCCCAAACTCTCCATGTTCAGAGTCCTGTGTCTCCCCA
  GAGGTTACTATAGAAACCCTACAGCCAGCAACAGAGCTCTCCAAGGCAGCAGAAGTGGAATCAGTGAAAG
  AGCAGCTGCCAGCTAAAGCATTGGAAACGATGGCAGAGCAGACCACTGATGTGGTGCACTCTCCATCCAC
  AGACACAACACCAGGCCCAGACACAGAGGCAGCACTGGCTAAAGACATAGAAGAGATCACCAAGCCAGAT
  GTGATATTGGCAAATGTCACGCAGCCATCTACTGAATCGGATATGTTCCTGGCCCAGGACATGGAACTAC
  TCACAGGAACAGAGGCAGCCCACGCTAACAATATCATATTGCCTACAGAACCAGACGAATCTTCAACCAA
  GGATGTAGCACCACCTATGGAAGAAGAAATTGTCCCAGGCAATGATACGACATCCCCCAAAGAAACAGAG
  ACAACACTTCCAATAAAAATGGACTTGGCACCACCTGAGGATGTGTTACTTACCAAAGAAACAGAACTAG
  CCCCAGCCAAGGGCATGGTTTCACTCTCAGAAATAGAAGAGGCTCTGGCAAAGAATGATGAGTCCTCTGC
  AGAAATACCTGTGGCTCAGGAGACAGTGGTCTCAGAAACAGAGGTGGTCCTGGCAACAGAAGTGGTACTG
  CCCTCAGATCCCATAACAACATTGACAAAGGATGTGACACTCCCCTTAGAAGCAGAGAGACCGTTGGTGA
  CGGACATGACTCCATCTCTGGAAACAGAAATGACCCTAGGCAAAGAGACAGCTCCACCCACAGAAACAAA
  TTTGGGCATGGCCAAAGACATGTCTCCACTCCCAGAATCAGAAGTGACTCTGGGCAAGGACGTGGTTATA
  CTTCCAGAAACAAAGGTGGCTGAGTTTAACAATGTGACTCCACTTTCAGAAGAAGAGGTAACCTCAGTCA
  AGGACATGTCTCCGTCTGCAGAAACAGAGGCTCCCCTGGCTAAGAATGCTGATCTGCACTCAGGAACAGA
  GCTGATTGTGGACAACAGCATGGCTCCAGCCTCCGATCTTGCACTGCCCTTGGAAACAAAAGTAGCAACA
  GTTCCAATTAAAGACAAAGGAACTGTACAGACTGAAGAAAAACCACGTGAAGACTCCCAGTTAGCATCTA
  TGCAGCACAAGGGACAGTCAACAGTACCTCCTTGCACGGCTTCACCAGAACCAGTCAAAGCTGCAGAACA
  AATGTCTACCTTACCAATAGATGCACCTTCTCCATTAGAGAACTTAGAGCAGAAGGAAACGCCTGGCAGC
  CAGCCTTCTGAGCCTTGCTCAGGAGTATCCCGGCAAGAAGAAGCAAAGGCTGCTGTAGGTGTGACTGGAA
  ATGACATCACTACCCCGCCAAACAAGGAGCCACCACCAAGCCCAGAAAAGAAAGCAAAGCCTTTGGCCAC
  CACTCAACCTGCAAAGACTTCAACATCGAAAGCCAAAACACAGCCCACTTCTCTCCCTAAGCAACCAGCT
  CCCACCACCTCTGGTGGGTTGAATAAAAAACCCATGAGCCTCGCCTCAGGCTCAGTGCCAGCTGCCCCAC
  ACAAACGCCCTGCTGCTGCCACTGCTACTGCCAGGCCTTCCACCCTACCTGCCAGAGACGTGAAGCCAAA
  GCCAATTACAGAAGCTAAGGTTGCCGAAAAGCGGACCTCTCCATCCAAGCCTTCATCTGCCCCAGCCCTC
  AAACCTGGACCTAAAACCACCCCAACCGTTTCAAAAGCCACATCTCCCTCAACTCTTGTTTCCACTGGAC
  CAAGTAGTAGAAGTCCAGCTACAACTCTGCCTAAGAGGCCAACCAGCATCAAGACTGAGGGGAAACCTGC
  TGATGTCAAAAGGATGACTGCTAAGTCTGCCTCAGCTGACTTGAGTCGCTCAAAGACCACCTCTGCCAGT
  TCTGTGAAGAGAAACACCACTCCCACTGGGGCAGCACCCCCAGCAGGGATGACTTCCACTCGAGTCAAGC
  CCATGTCTGCACCTAGCCGCTCTTCTGGGGCTCTTTCTGTGGACAAGAAGCCCACTTCCACTAAGCCTAG
  CTCCTCTGCTCCCAGGGTGAGCCGCCTGGCCACAACTGTTTCTGCCCCTGACCTGAAGAGTGTTCGCTCC
  AAGGTCGGCTCTACAGAAAACATCAAACACCAGCCTGGAGGAGGCCGGGTCCAGATAGTATCCAAAAAAG
  TGAGCTACAGCCATATTCAATCCAAGTGTGGTTCCAAGGACAATATTAAGCATGTCCCTGGAGGTGGCAA
  TGTTCAGATTCAGAACAAGAAAGTGGACATATCCAAGGTCTCCTCCAAGTGTGGGTCCAAAGCTAATATC
  AAGCACAAGCCTGGTGGAGGAGATGTCAAGATTGAAAGTCAGAAGTTGAACTTCAAGGAGAAGGCCCAAG
  CCAAAGTGGGATCCCTTGATAACGTGGGCCACTTGCCTGCAGGAGGTGCCGTGAAGACTGAGGGCGGTGG
  CAGTGAGGCCCTTCCGTGTCCAGGCCCCCCCGCTGGGGAGGAGCCAGTCATCCCTGAGGCTGCGCCTGAC
  GCTGGCGCCCCTACTTCAGCCAGTGGCCTCAGTGGCCACACCACCCTGTCAGGGGGTGGTGACCAAAGGG
  AGCCCCAGACCTTGGACAGCCAGATCCAGGAGACAAATTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001205332
• Insert Size: 3261 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001205332.1, NP_001192261.1
• RefSeq Size: 5556 bp
• RefSeq ORF: 3261 bp
• Locus ID: 17758
• UniProt ID: P27546
• Cytogenetics: 9 59.83 cM
• Gene Summary: Non-neuronal microtubule-associated protein. Promotes microtubule assembly.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) differs in the 3' UTR and has multiple coding region differences, compared to variant 1, one of which results in a frameshift. The resulting protein (isoform 4) has a distinct C-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.