AU040320 (NM_001035526) Mouse Untagged Clone

CAT#: MC229348

AU040320 (untagged) - Mouse expressed sequence AU040320 (AU040320), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: AU040320
• Synonyms: A730047D20Rik; AAVR; Kiaa0319l
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229348 representing NM_001035526
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAAGAGACTGGGAGTCAAGCCAAGTCCCGCTTCCTGGGTTTTGCCAGGATATTGTTGGCAGACAT
  CAGTGAAGCTGCCGAGAAGCCTGTACCTGCTTTACAGTTTCTTCTGCTTCAGCGTTCTGTGGTTGTCAAC
  AGATGCTGATGAGAGCAGATGCCAACAGGGGAAGACACTTTATGGAGCTGGCTTGAGAACTGAGGGAGAA
  AATCACCTCCGGCTTCTTGCAGGAAGCCTGCCTTTCCACGCCTGTCGGGCTGCCTGCTGCCGGGACTCTG
  CCTGCCACGCTCTATGGTGGCTGGAAGGGATGTGCTTTCAGGCTGACTGCAGTAAGCCCCAGAGCTGCCA
  GCCTTTTAGGACAGACTCTTCCAATTCCATGCTGATCATTTTTCAAAAATCCCAAACTACAGATGATTTG
  GGCCTTCTGCCTGAAGATGATGAACCACATCTTCTGAGGCTAGGCTGGGGCAGGACATCGTGGAGGAGGC
  AGAGCCTTCTTGGGGCTCCCCTCACCCTTTCTGTACCCTCTAGTCACCACCAGAGCTTACTCAGGGATCG
  GCAGAAGAGAGATCTCAGTGTGGTACCTACACATGGAGCGATGCAGCATTCTAAAGTGAATCACTCGGAG
  GAAGCAGGTGCTCTGAGTCCCACCTCTGCAGAGGTCCGCAAAACCATTACAGTTGCCGGTTCCTTCACCA
  GTAACCACACTACACAGACTCCTGAGTGGCCCAAGAATGTGTCCATCCATCCTGAACCATCCGAGCACTC
  CAGTCCTGTATCTGGTACTCCGCAAGTAAAAAGCACTGAGCACAGTCCAACTGATGCCCCTCTGCCAGTG
  GCCCCCTCCTACAGCTATGCCACCCCCACGCCCCAGGCCTCTTCTCAGAGCACCTCAGCACCACACCCAG
  TTGTAAAGGAGCTGGTGGTGTCTGCTGGGAAGAGCGTCCAGATCACCCTGCCTAAGAATGAAGTTCAGTT
  AAATGCCTTCGTCCTTCCAGAAGCAGAGCCAGGAGAAACCTACACCTACGACTGGCAGCTGATCACTCAT
  CCTACAGACTACAGTGGAGAGGTGGAGAGGAAACATTCCCAGAGCCTCCAACTGTCCAAGCTGACTCCAG
  GCCTGTACGAATTCAAGGTGACTGTGGATGGCCAGAATGCCCATGGGGAAGGCTACGTGAATGTGACAGT
  GAAACCAGAGCCCCGTAAGAACCGGCCTCCCGTTGCTGTGGTGTCACCTCAGTTCCAGGAGATCTCGCTG
  CCAACCACTTCTACCATCATTGATGGCAGCCAGAGCACGGATGACGATAAAATTGTCCAGTACCACTGGG
  AAGAGCTTAAGGGGCCCCTGAGAGAAGAGAAGATCTCTGAAGACACAGCCATACTAAAACTTAGTAAGCT
  CGTCCCGGGGAACTACACCTTCAGCTTAACTGTTGTCGACTCTGACGGGGCTACCAACTCCACCACTGCA
  AGCCTGACTGTGAACAAAGCTGTGGACTACCCTCCCGTGGCCAATGCAGGCCCCAACCAAGTGATCACCC
  TGCCTCAGAACTCCATCACCCTCTTTGGAAACCAGAGCACGGATGACCACGGCATCACCAGCTATGAGTG
  GTCGCTCAGCCCGAGCAGCAAAGGGAAGGTGGTGGAGATGCAGGGAGTTAGAACGCCAGCCCTGCAGCTG
  TCCGCAATGCAAGAAGGAGACTATACCTACCAGCTCACAGTGACTGACACCGCAGGACAACAGGCCACCG
  CCCAAGTGACTGTGATTGTGCAGCCTGAGAACAACAAGCCTCCTCAGGCAGATGCAGGCCCAGACAAAGA
  GCTGACCCTGCCCGTGGACAGCACAACCCTGGACGGCAGCAAGAGCACAGATGACCAGAGAGTCGTCTCT
  TACCTTTGGGAGCAGAGTCGGGGACCTGACGGGGTGCAGCTGGAGAATGCCAACAGCAGTGTCGCCACTG
  TGACTGGGCTGCAAGTCGGGACTTATGTATTCACCTTGACTGTCAAAGATGAGAGGAACCTACAGAGCCA
  GAGCTCCGTTAATGTCATTGTCAAAGAAGAAATAAACAAACCGCCAGTAGCCAAGATCGCTGGGAACGTG
  GTGGTGACCTTGCCCACGAGCACAGCTGAGCTGGATGGCTCGAGGTCCTCAGATGACAAGGGGATAGTCA
  GCTACCTGTGGACTCGAGATGAGACGAGCCCAGCCGCAGGGGAGGTGCTGAATCACTCTGACCACCACCC
  TGTCCTCTTCCTCTCCAACCTGGTGGAGGGGACCTACACGTTTCACCTGAAAGTGACAGATGCAAAGGGC
  GAGAGCGACACAGACCGGACGACAGTGGAAGTGAAGCCTGACCCCAGGAAAAGCAACCTAGTGGAGATCA
  TCTTGGATGTGAACGTCAGTCAGCTGACTGAGAGGCTGAAGGGGATGCTCATCCGCCAGATTGGGGTCCT
  CCTGGGGGTGCTGGATTCCGACATCATTGTGCAAAAGATTCAGCCGTACACGGAGCAGAGCACCAAGATG
  TTGTTTTTTGTTCAGAACGACCCTCCCCACCAGCTCTTCAAAGGCCATGAGGTGGCAGCCATGCTCAAGA
  GCGAGCTGCAGAAGCAGAAGGCTGACTTCCTCATCTTCAGAGCCCTGGAAATCAGCACAGTCACATGTCA
  GCTGAACTGTTCTGACCATGGCCACTGTGACTCATTCACCAAGCGCTGTGTCTGTGACCCGTTTTGGATG
  GAGAATTTCATCAAGGTGCAGCTGAGGGATGGAGACAGCAACTGTGAATGGAGCGTGCTCTACGTCATCA
  TTGCCTCCTTTGTCATTGTTGTTGCCTTGGGGATCCTGTCATGGACTACAATCTGCTGCTGCAAGAGGCA
  AAAAGGAAAACCCAAGAGGAAAAGCAGATACAAGATCCTGGATGCCACAGATCAGGAGAGCCTGGAGCTG
  AAACCAACCTCCCGAGCAGGCAGCAAACAGAAAGGCCCCACGCTGAGCAGCAGCCTGATGCATTCTGAAT
  CGGAGCTGGACAGCGACGATGCCATCTTCACATGGCCAGACCGGGAGAAGGGCAAACTACTGGCCCTCCA
  CTGGTGTAGATCCCAAAGCCTGCAGCATATTGGCTGGTGGTGTAGGGCCCCAGTCTGCCATGAAACCTCA
  TCTACCAACAGCACACCTGGCTCTGACTCCGGCTTCTGTAAAGGCGGGAAGAACCTCAGCTCAGACTACA
  GAACCAGGACGAAGTCAGCTTTGGTCCATGGGAAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001035526
• Insert Size: 3258 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001035526.1, NP_001030603.1
• RefSeq Size: 3815 bp
• RefSeq ORF: 3258 bp
• Locus ID: 100317
• UniProt ID: Q8K135
• Cytogenetics: 4 D2.2
• Gene Summary: Possible role in axon guidance through interaction with RTN4R.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) differs in the 5' UTR, compared to variant 2. Both variants 1 and 2 encode the same isoform.