Daam1 (NM_001286452) Mouse Untagged Clone

CAT#: MC229332

Daam1 (untagged) - Mouse dishevelled associated activator of morphogenesis 1 (Daam1), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Daam1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229332 representing NM_001286452
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCACGGAAGAGAGGCGGCCGGGGGATTTCATTTATCTTTTGCTGTTTCCGAAACAATGACCATC
  CAGAAATCACATATCGGCTTAGGAATGATAGCAACTTTGCACTTCAGACAATGGAGCCGGCGCTGCCCAT
  GCCCCCTGTGGAGGAGCTCGATGTCATGTTCAGTGAACTTGTGGATGAACTTGACCTCACAGACAAACAC
  AGGGAAGCCATGTTTGCACTTCCAGCAGAGAAAAAGTGGCAGATATACTGTAGCAAGAAAAAGGACCAGG
  AAGAAAACAAGGGAGCAACCAGTTGGCCCGAGTTCTACATTGATCAGCTCAATTCCATGGCTGCTAGGAA
  ATCTCTGCTGGCTTTAGAGAAGGAAGAAGAGGAGGAGAGGAGTAAAACCATCGAGAGTCTGAAGACAGCA
  CTGAGAACCAAGCCCATGAGGTTTGTAACCAGATTCATCGACTTGGATGGCCTGTCATGTATTCTCAACT
  TTCTGAAGACCATGGACTATGAGACCTCTGAGTCTCGGATACACACCTCCCTCATTGGCTGTATAAAGGC
  ACTAATGAACAACTCTCAAGGCCGGGCCCATGTCCTGGCTCATTCTGAGAGTATCAATGTAATTGCTCAG
  AGTCTGAGCACAGAGAACATTAAAACAAAGGTGGCCGTGCTGGAAATCCTGGGCGCTGTGTGCCTGGTTC
  CTGGGGGCCACAAGAAAGTTCTGCAAGCTATGCTGCACTACCAGAAGTATGCCAGCGAGAGAACCCGCTT
  CCAGACGTTAATTAATGACTTGGATAAAAGCACTGGGCGTTATCGGGATGAAGTAAGTCTCAAGACGGCC
  ATCATGTCCTTCATCAATGCAGTGCTGAGCCAGGGAGCCGGAGTGGAGAGTTTGGACTTCAGGCTCCATC
  TTCGCTATGAATTTCTGATGTTAGGAATTCAACCTGTTATAGATAAATTAAGGGAACATGAAAATTCAAC
  ACTAGACAGGCATTTAGACTTTTTTGAAATGCTCCGAAATGAAGATGAACTAGAATTTGCCAAAAGATTT
  GAACTGGTTCACATAGACACAAAAAGCGCCACTCAGATGTTTGAGCTGACCAGGAGGAGGCTAACCCACA
  GCGAAGCCTACCCCCACTTCATGTCCATTCTGCACCATTGCCTCCAGATGCCTTACAAGAGGAGTGGTAA
  CACAGTTCAGTACTGGCTGCTGTTAGACAGAATCATACAGCAGATAGTTATCCAGAATGACAAAGGGCAG
  GATCCCGACTCCACACCTCTGGAAAACTTTAATATCAAGAACGTCGTAAGAATGTTGGTTAATGAAAATG
  AAGTTAAGCAATGGAAAGAGCAGGCAGAGAAGATGAGGAAAGAGCACAATGAGCTACAACAGAAGCTGGA
  GAAGAAAGAGAGAGAATGTGATGCTAAGACCCAAGAGAAGGAAGAAATGATGCAGACCTTAAACAAGATG
  AAAGAGAAGCTGGAAAAGGAGACAACTGAGCACAAGCAAGTTAAGCAGCAGGTGGCAGACCTCACGGCGC
  AGCTCCACGAGCTGAACAGGAGGGCCGTCTGTGCAGCAGTCCCAGGAGGACCCTCACCCGGAGCCCCTGG
  AGGACCCTTTCCTTCCTCTGGGTTGGGGTCTCTTCTCCCTCCCCCACCACCCCCACTCCTTTCAGGTGGA
  GCACTTCCTCCTCCACCACCTCCCCTCCCTCCTGGGGGTCCTCCTCCTCCCCCAGGGCCTCCTCCCTTAG
  GAGGAGTTCTGCCACCTCCTGGTGCTCCAGTGAGTCTAACACTCAAGAAGAAGAATATTCCCCAGCCCAC
  CAATGCCCTGAAATCCTTCAACTGGTCCAAGTTGCCTGAGAACAAATTGGATGGAACAGTATGGACTGAA
  ATTGATGATACCAAAGTCTTCAAAATTCTAGACCTTGAAGACCTAGAAAGAACGTTCTCTGCCTACCAAA
  GACAGCAGAAAGAAGCAGATGCCATTGATGACACACTGAGTTCCAAACTTAAAGTCAAAGAGCTGTCAGT
  GATTGATGGTCGGAGAGCTCAGAATTGCAACATCCTTCTGTCGAGGTTGAAATTATCCAATGATGAAATC
  AAGCGGGCAATTCTAACAATGGATGAACAGGAGGATCTGCCCAAGGACATGTTAGAACAGCTTTTGAAAT
  TCGTTCCTGAGAAAAGCGACATCGACCTGCTAGAGGAACACAAGCATGAGCTGGATCGGATGGCCAAGGC
  TGACCGCTTCCTGTTTGAGATGAGCAGAATTAATCATTACCAGCAAAGACTGCAATCACTGTACTTCAAA
  AAGAAGTTTGCAGAGCGTGTGGCAGAAGTGAAGCCCAAAGTAGAAGCGATTCGCTCTGGCTCTGAGGAGG
  TGTTCAGGAGCCGCGCCCTCAAGCAGCTCCTGGAGGTGGTCTTGGCTTTTGGAAATTATATGAATAAAGG
  ACAAAGAGGCAATGCATATGGATTCAAGATCTCCAGCCTCAACAAGATAGCGGATACCAAATCCAGTATT
  GACAAGAACATTACTCTTTTGCACTATCTAATAACTATTGTGGAGAATAAGTACCCCAAAGTCCTCAACC
  TAAGCGAAGAGTTGCGGGATATTCCTCAAGCAGCGAAAGTAAACATGACTGAGCTGGACAAGGAGATCAG
  CACCCTGAGGAGTGGCCTGAAAGCTGTAGAGACGGAGCTGGAGTATCAGAAGTCTCAGCCCCCACAGCCT
  GGAGACAAGTTCGTGTCTGTTGTCAGCCAGTTCATCACCCTGGCTAGCTTCAGCTTCTCAGATGTTGAAG
  ATCTTCTAGCAGAAGCTAAAGAGCTGTTCACTAAAGCAGTGAAGCACTTTGGGGAAGAGGCTGGCAAGAT
  CCAGCCAGATGAGTTCTTCGGGATCTTTGATCAGTTTCTTCAGGCCGTGGCCGAAGCGAAACAGGAAAAT
  GAGAACATGAGAAAAAGGAAGGAGGAGGAGGAACGGCGGGCTCGCCTGGAAGCTCAGCTCAAAGAGCAGC
  GGGAGAGGGAGCGGAAGGTGAGGAAGGCCAAGGAGAGCAGTGAAGAGAGTGGAGAGTTTGACGACCTGGT
  CTCGGCGCTGCGCTCAGGAGAGGTGTTCGACAAAGACCTTTCAAAACTCAAACGGAACCGGAAACGCATT
  TCCAACCAGGTGACGGACAGCAGCCGGGAGCGACCAATCACAAAACTTAATTTTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001286452
• Insert Size: 3207 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286452.1, NP_001273381.1
• RefSeq Size: 6028 bp
• RefSeq ORF: 3207 bp
• Locus ID: 208846
• UniProt ID: Q8BPM0
• Cytogenetics: 12 C3
• Gene Summary: Binds to disheveled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments (By similarity). Involved in building functional cilia. Involved in the organization of the subapical actin network in multiciliated epithelial cells (By similarity). Together with DAAM2, required for myocardial maturation and sarcomere assembly (PubMed:26526197).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR and lacks an in-frame exon in the central coding region, compared to variant 1. The encoded isoform (b) is shorter, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.