Srgap1 (NM_001242411) Mouse Untagged Clone

CAT#: MC229328

Srgap1 (untagged) - Mouse SLIT-ROBO Rho GTPase activating protein 1 (Srgap1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Srgap1
• Synonyms: 4930572H05Rik; Arhgap13
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229328 representing NM_001242411
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCACCCCGAGCAGATTCAAGAAGGACAAAGAGATCATAGCCGAGTATGAAAGTCAAGTCAAAGAAA
  TCCGAGCCCAGCTGGTAGAACAACAAAAATGCCTGGAGCAGCAGACAGAGATGCGAGTTCAGCTGCTCCA
  GGACCTACAAGATTTCTTCCGGAAAAAAGCCGAAATTGAGACGGAATATTCTCGGAACCTGGAGAAGCTG
  GCAGAAAGGTTCATGGCCAAAACAAGAAGCACTAAGGACCATCAACAGTTCAAGAAAGACCAAAACCTGT
  TGTCTCCAGTGAACTGCTGGTATTTGCTCCTGAACCAAGTGAGAAGAGAGAGCAAAGACCACGCCACTTT
  GAGTGACATCTATCTGAACAATGTCATCATGCGGTTCATGCAGATAAGCGAGGACTCCACCCGGATGTTT
  AAAAAGAGCAAAGAGATTGCGTTCCAGCTTCACGAGGACCTCATGAAGGTTCTGAATGAGCTCTACACGG
  TGATGAAAACCTACCACATGTACCACTCAGAGAGCATCAGTGCAGAGAGTAAGCTGAAAGAAGCCGAGAA
  ACAAGAGGAGAAGCAGATTGGGAGGTCCGGCGACCCAGTCTTCCACATTAGGCTGGAAGAGAGGCATCAG
  AGGCGGAGCTCTGTGAAGAAAATTGAAAAAATGAAGGAAAAGAGACAAGCAAAATATTCAGAAAATAAGC
  TGAAATCGATCAAAGCCCGCAACGAGTATCTCCTAACACTTGAAGCAACCAACGCCTCCGTGTTCAAGTA
  TTACATCCATGATCTTTCAGACTTAATAGACTGCTGTGATCTCGGCTACCATGCAAGTCTGAACAGAGCC
  CTCAGAACGTACCTCTCGGCGGAGTACAACCTTGAAACCTCCAGACACGAAGGCTTAGACATCATTGAAA
  ACGCTGTGGACAATTTAGAGCCCAGGAGTGACAAGCAGAGGTTCATGGAGATGTACCCTGCGGCATTCTG
  CCCGCCAATGAAATTCGAGTTTCAGTCTCACATGGGTGATGAGGTGTGTCAGGTCAGCGCCCAGCAGCCC
  GTCCAGGCGGAGCTCATGCTCAGGAACCAACAGCTGCAGTCCCGACTGGCCACACTCAAGATCGAGAGCG
  AGGAGGTCAAGAAAACGACAGAAGCCACCCTCCAGACGATCCAAGATATGGTCACCATTGAGGACTATGA
  TGTGTCTGAGTGTTTCCAGCACAGCCGATCCACAGAGTCTGTGAAGTCCACTGTGTCAGAAACCTACCTG
  AGTAAACCCAGCATCGCCAAGAGACGAGCCAACCAGCAAGAAACGGAGCAGTTCTACTTCATGAAACTTC
  GAGAGTTCCTGGAAGGCAGTAACCTCATCACAAAGCTGCAGGCTAAGCATGACTTGCTGCAGAGGACTCT
  TGGAGAAGGCCATAGAGCTGAATATATGACTACAAGCCGGGGACGAAGGAACTCTCACGCAAGACATCAG
  GACTCAGGCCAGGTTATCCCTCTCATTGTGGAAAGCTGCATCCGATTCATTAATCTCTATGGCCTTCAGC
  ACCAGGGGATTTTCAGAGTGTCTGGCTCCCAGGTGGAAGTGAACGATATCAAGAATTCTTTCGAGAGAGG
  TGAGAACCCTCTGTCCGATGAGCAGAGCAACCACGATATCAACTCGGTGGCCGGCGTGCTGAAGCTGTAC
  TTCCGAGGGCTGGAAAACCCCCTCTTTCCTAAGGAAAGGTTTACTGACCTGATTTCCTGTATCAGAATAG
  ATAACCTCTATGAGAGGGCGCTCCACATCCGCAAACTCCTCCTGACCTTGCCCAGGTCGGTCCTTATAGT
  GATGAGGTACCTGTTTGCCTTCCTCAATCACCTCTCCCAGTACAGTGACGAGAACATGATGGACCCTTAC
  AATCTGGCCATTTGCTTCGGCCCAACACTGATGCCGGTTCCAGAGATACAGGACCAAGTGTCTTGCCAGG
  CACACGTGAACGAGATTGTGAAGACCATCATCATCCATCACGAGACTATTTTCCCAGATGCTAAAGAACT
  GGACGGCCCCGTGTATGAGAAATGCATGGCCGGAGGCGACTACTGCGACAGCCCCTACAGTGAGCATGGT
  ACATTGGAGGAGGTGGACCAAGACGCCGGCACAGAGCCCCACACCAGTGAAGACGAGTGTGAGCCCATCG
  AAGCCATAGCCAAGTTTGACTACGTCGGGCGGTCAGCTAGAGAGCTGTCCTTCAAGAAGGGTGCATCCCT
  GCTGCTGTATCACCGAGCCTCCGAGGATTGGTGGGAAGGCAGACACAACGGGATCGATGGCCTTGTGCCT
  CATCAGTACATAGTTGTGCAAGATATGGACGATACATTTTCAGACACGCTGAGCCAAAAGGCCGACAGCG
  AAGCCAGCAGCGGGCCAGTCACTGAAGACAAGTCCTCATCCAAGGACATGAACTCCCCGACTGACCGCCA
  TTCTGATAGCTATTTAGCCAGACAAAGAAAGAGAGGAGAGCCACCCCCTCCGGGAAGACGTCCCGGCAGG
  ACCAGCGATGGCCATTGTCCCCTGCATCCCCCGCATGCTCTGTCTAACTCCTCAATTGATCTGGGATCCC
  CAAACCTAGCCAGTCACCCCAGGGGCCTGTTGCAGAACCGTGGCCTCAATAATGACAGTCCTGAGAGGCG
  GCGCCGGCCGGGCCACGGCAGCCTGACCAATATCAGCCGGCATGACTCCCTCAAGAAGATCGACAGCCCT
  CCTATCCGGAGGTCCACATCATCAGGGCAGTACACAGGCTTCAATGACCACAAGCCACTGGACCCCGAGA
  CGATTGCTCAGGATATTGAGGAGACCATGAACACTGCTTTGAACGAACTCCGTGAACTCGAGAGGCAGAG
  CACAGTGAAGCACGCCCCCGATGTCGTGCTAGACACTTTGGAGCAAGTGAAGAACTCGCCCACCCCTGCG
  ACTTCCACAGAATCACTCAGCCCACTGCACAATGTCGCCCTCAGGGGCTCAGAGCCACAGATTCGCCGGA
  GCACCAGCTCCTCCAGCGAGACAATGAGTACTTTCAAGCCTATGGTGGCGCCCCGGATGGGCGTCCAGCT
  GAAGCCCCCAGCCCTTAGGCCAAAGCCTGCTGTTCTTCCAAAAACAAACCCTACGATGGGACCTGCCGCA
  CCTTCCCAGGGTCCCACAGACAAGTCTTGCACGATGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001242411
• Insert Size: 3189 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001242411.1, NP_001229340.1
• RefSeq Size: 7718 bp
• RefSeq ORF: 3189 bp
• Locus ID: 117600
• UniProt ID: Q91Z69
• Cytogenetics: 10 D2
• Gene Summary: GTPase-activating protein for RhoA and Cdc42 small GTPases. Together with CDC42 seems to be involved in the pathway mediating the repulsive signaling of Robo and Slit proteins in neuronal migration. SLIT2, probably through interaction with ROBO1, increases the interaction of SRGAP1 with ROBO1 and inactivates CDC42 (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate coding exon, that causes a frameshift; however, it contains an alternate downstream exon that restores the reading frame. The resulting isoform (2) has a different internal segment compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.