Grip1 (NM_001277292) Mouse Untagged Clone

CAT#: MC229326

Grip1 (untagged) - Mouse glutamate receptor interacting protein 1 (Grip1), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Grip1
• Synonyms: 4931400F03Rik; eb; GRIP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229326 representing NM_001277292
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGGCTGGAAGAAGAACATCCCCATCTGCTTACAAGCGGAGGAGCAGGAGAGAGATGAGAGTCCCT
  ACACTAAATCTGCCAGCCAGACAAAGCCGCCCGATGGAGCATTGGCTGTGAGGAGACAGAGCATCCCAGA
  GGAATTCAAGGGCTCCACAGTGGTGGAGTTGATGAAGAAGGAGGGAACCACTCTTGGCCTGACGGTATCG
  GGAGGCATTGATAAAGATGGCAAGCCAAGAGTGTCCAACCTGCGGCAGGGAGGAATCGCTGCCAGAAGTG
  ACCAGCTGGATGTGGGCGACTACATCAAGGCGGTGAATGGGATCAACCTGGCCAAGTTCCGCCACGATGA
  GATCATCAGCCTGCTGAAAAATGTCGGGGAAAGAGTGGTCCTGGAGGTCGAGTACGAGCTTCCACCGGTC
  TCTGTACAAGGATCCAGTGTTATGTTCCGAACTGTGGAGGTCACGCTGCACAAAGAAGGCAACACCTTTG
  GTTTTGTCATCCGAGGGGGAGCGCATGATGACAGGAACAAGTCCCGTCCGGTTGTGATAACCTGTGTTCG
  TCCTGGAGGGCCTGCTGACAGAGAGGGCACCATCAAACCTGGAGACAGGTTGCTCAGCGTGGATGGAATT
  CGGCTCCTGGGAACCACCCATGCCGAGGCCATGAGCATCCTTAAACAGTGCGGACAAGAAGCCACGCTGC
  TGATAGAATACGATGTCTCTGTGATGGATTCTGTGGCGACAGCATCCGGGCCACTACTAGTTGAAGTTGC
  CAAAACTCCAGGTGCCAGCCTTGGGGTTGCCCTAACTACCTCCGTGTGCTGTAACAAGCAGGTCATTGTC
  ATAGACAAAATCAAATCTGCAAGCATTGCGGACAGATGTGGCGCGCTACACGTGGGAGACCACATCCTCT
  CCATTGACGGCACGAGTATGGAGTACTGTACCCTCGCAGAAGCGACCCAGTTCCTGGCCAATACCACTGA
  CCAGGTCAAGCTGGAGATTCTCCCACACCATCAGACCCGCCTGGCCCTAAAGGGCCCTGACCATGCGGCT
  ATGGTGCCCTCATCTTCTCCTACCTCCATGAGTGCGTACAGTCTGAGTTCCCTGAACATGGGGACTTTAC
  CTCGAAGCCTCTACTCCACCAGCCCACGAGGAACCATGATGAGGAGGAGACTGAAAAAGAAAGACTTCAA
  AAGCTCACTGTCTTTAGCCTCCAGCACTGTGGGGTTGGCTGGCCAGGTCGTTCACACTGAAACCACAGAG
  GTTGTGCTGACGGCTGACCCTGTCACGGGCTTCGGAATCCAACTGCAGGGCAGCGTGTTTGCCACAGAGA
  CGCTCTCCTCTCCGCCTCTGATTTCCTATATTGAAGCTGACAGCCCAGCAGAGAGATGTGGTGTGCTACA
  GATTGGAGACAGAGTCATGGCCATTAATGGAATCCCAACAGAAGACAGCACCTTCGAGGAAGCCAATCAA
  CTCCTGAGAGACTCTTCCATCACGAGCAAAGTCACACTAGAAATCGAGTTTGATGTTGCAGAGTCTGTCA
  TCCCAAGTAGTGGAACATTTCATGTAAAACTGCCCAAGAAGCACAGCGTGGAACTTGGAATAACCATCAG
  TTCGCCATCCAGTAGAAAACCGGGGGACCCCCTTGTCATTTCAGATATCAAGAAAGGCAGTGTGGCACAC
  AGAACCGGAACTCTGGAACTGGGAGACAAACTGCTTGCGATAGATAACATCCGGCTGGATAACTGTTCCA
  TGGAAGATGCGGTCCAGATCCTCCAGCAGTGTGAAGACCTGGTGAAGCTCAAAATCCGCAAAGATGAAGA
  TAACTCAGACGAGCAAGAGAGTTCCGGAGCGATTATTTACACGGTGGAGCTGAAGCGCTATGGGGGGCCC
  CTTGGCATCACAATTTCTGGAACTGAAGAGCCGTTTGATCCTATTATCATCTCGAGCCTCACTAAAGGGG
  GATTAGCTGAAAGGACTGGAGCGATCCACATCGGAGATAGAATCCTAGCCATCAATAGCAGCAGCTTGAA
  GGGGAAGCCTCTGAGTGAAGCCATCCACTTGCTCCAGATGGCAGGAGAGACTGTCACCCTGAAAATTAAG
  AAACAGACAGATGCCCAATCTGCATCAAGTCCCAAGAAGTTCCCCATCCCTGGCCACTCGGGGGACCTAG
  GAGATGGTGAGGAGGACCCCTCCCCAATACAGAAACCTGGCAAGCTCTCCGATGCGTACCCCTCCACGGT
  GCCCAGCGTGGACAGTGCTGTGGACTCCTGGGATGGGTCTGGAATAGATGCCAGCTATGGGAGTCAAGGC
  TCAACTTTTCAGACTTCAGGATACAATTACAACACCTATGATTGGAGGAGTCCAAAGCAAAGAACCAGCC
  TGTCCCCAGTCCCCAAGCCTCGAAGCCAGACGTACCCAGATGTGGGCCTGAGTAATGAAGACTGGGATCG
  ATCCACAGCCAGTGGCTTTGTAGGGGCTTCTGACAGTGCAGATGCTGAACAAGAGGAAAACTTCTGGTCT
  CAAGCATTGGAGGACCTGGAGACCTGCGGCCAGTCGGGGATCCTGAGAGAGCTTGAGGCAACAATCATGT
  CGGGGAGTACTATGAGTTTGAATCATGAGGCTCCAATGGCTCGCAGTCAGCTGGGGCGACAGGCCAGCTT
  CCAGGAACGGAGCAGTTCACGGCCACACTATAGCCAAACAACTCGCAGCAACACCCTGCCCTCAGACGTG
  GGCAGAAAGTCTGTAACCCTGCGGAAAATGAAGCAAGAAATAAAGGAGATCATGTCCCCAACTCCGGTGG
  AGCTACACAAGGTGACCTTATACAAGGACTCTGGCATGGAGGACTTCGGGTTCAGTGTGGCAGATGGCCT
  GCTGGAGAAAGGCGTGTATGTCAAAAATATCCGCCCAGCTGGGCCAGGTGATGTTGGGGGCTTGAAGCCC
  TACGACAGGCTCTTACAGGTAAATCACGTGCGGACGAGAGACTTTGACTGCTGCCTGGTGGTGCCTCTCA
  TAGCTGAATCTGGCAACAAGCTGGACCTGGTTATTAGCAGAAATCCACTGGCCTCCCAGAAGTCGATAGA
  ACAGCCGGCTCTGCCCAGCGACTGGAGCGAACAGAACAGCGCTTTCTTCCAGCAACCCAGCCACGGTGGT
  AATCTAGAGACACGAGAACCCACTAACACACTATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001277292
• Insert Size: 3186 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001277292.1, NP_001264221.1
• RefSeq Size: 5161 bp
• RefSeq ORF: 3186 bp
• Locus ID: 74053
• UniProt ID: Q925T6
• Cytogenetics: 10 67.33 cM
• Gene Summary: This gene encodes a protein containing multiple PDZ (post synaptic density protein, Drosophila disc large tumor suppressor, and zonula occludens-1 protein) domains. The encoded protein acts as a mediator between cytoskeletal and membrane proteins, particularly in neuronal cells, and facilitates complex formation at the cell membrane. Mutation of this gene can cause embryonic lethality resulting from defects of the dermo-epidermal junction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]
  Transcript Variant: This variant (4) contains multiple differences, compared to variant 1, including the lack of multiple exons. It represents use of an alternate promoter and initiates translation at an alternate start codon. The encoded isoform (4) is shorter and has a distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.