Ripor2 (NM_001286100) Mouse Untagged Clone

CAT#: MC229316

Fam65b (untagged) - Mouse family with sequence similarity 65, member B (Fam65b), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ripor2
• Synonyms: Fam65b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229316 representing NM_001286100
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGTGGGATCCCAGTCCTTCTCTCCTGGCGGGCCCAATGGCATCATCCGGAGCCAGTCCTTCGCAG
  GTTTCAGTGGGCTGCAGGAAAGGCGGTCCAGGTGTAACTCCTTCATTGAAAATGCCTCGGCTCTCAAGAA
  GCCTCAGGCCAAGCTGAAGAAAATGCATAATCTTGGACACAAAAATAACAACACTCCCAAGGAGCCTCAG
  CCGAAGAGAGTGGAGGAGGTCTACCGGGCCTTGAAGAACGGGCTTGATGAGTACCTGGAGTTTCACCAGA
  CGGAGCTGGACAAATTGACTGCTCAATTAAAAGATATGAAGAGAAACTCTCGCCTGGGTGTGCTGTATGA
  CCTAGACAAGCAAATTAAAACGATTGAGAGATACATGCGACGCCTGGAGTTTCACATTAGTAAGGTAGAC
  GAGCTCTATGAAGCCTATTGTATCCAACGGCGTCTCCAAGATGGTGCCAGCAAAATGAAGCAAGCCTTCG
  CGACGTCCCCTGCCAGCAAGGCTGCCCGGGAGAGTCTGTCTGAGATCAACCGCAGCTACAAGGAATACAC
  GGAGAACATGTGTGCCATTGAAGCGGAGTTGGAGAGTCTGCTGGGAGAATTCTCCATCAAGATGAAAGGT
  CTGGCTGGCTTTGCCCGCCTCTGTCCTGGAGATCAATATGAAATTTTCATGAAGTATGGTCGGCAGAGGT
  GGAAACTGAAGGGCAAAATAGAAGTGAATGGTAAACAGAGCTGGGATGGAGCAGAAACCGTCTTCCTGCC
  CCTCATAGTTGGGTTCATTTCAATCAAGGTCACAGAACTCAAAGGACTGGCGACTCACATCCTGGTAGGC
  AGTGTGACCTGCGAGACCAAAGAGCTGTTTGCAGCCCGACCCCAGGTGGTGGCTGTTGACATCAATGACC
  TTGGTACCATCAAACTGAACCTGGAAATCACCTGGTATCCCTTTGACGTGGAGGACACAACCCCATCGTC
  GGGCCCTGGGAACAAGACAGCTGCTCTCCAGCGAAGGATGTCCATGTACAGCCAGGGCACCCCAGAAACA
  CCTACCTTCAAAGACCAGTCCTTCTTTAGATGGTTGCGGCTGTCTGTCTTGAGTGCCTTGCGAGACACTT
  TCTTTGCCACGTTGCACCACAACCACTCTGTCGGTGACCTGCCTTCCCTCAGCCTGAACCCCAAGGCCCT
  GCTAGAATTCTATTCAAATCTGCCAGATGACATCTTTGAGAGTGGAAAGGCTGCAGAGGAGAAAAGGCCA
  CTGTCCCTCAGCTTCAGTGACCTGCAGGATGGAGACTGTGTCTTCACCTCCAGCTCAGCCACCTCCCCCT
  CCAGTTCACACTCAGCCCATCCAGAGATTACCATCACCCCCGCAGAGCTTACCCACAGCAGCCTGTCCTC
  ACAAAATGAGGGCACCGAGGACAGCAGCTCAGCGTCTTCCAGGAATTCCTTGGGTGAGGACCATGAGCCC
  AAGTCCCACCCAAAGAGTGATACGGTAGAGCCCGGGAAGCCGGGTGTGGCTACAAGGTCTGGTACCGAGA
  GCCTGTTTCTGGAGAGTAGTGTGGCCGAGGCTCTCCTGCAGGAATCGGATGAGGCCTCTGAGCTCAAGCC
  CGTGGAACTGGACACTTTTGAAGGAAACATCACAAAGCAACTGGTGAAGAGGCTCACCTCGGCTGAGGGG
  CCCGTCACTACTGACAAGCTTTTCTTTGAAGGCTCTGTTGGTAGCGAGTCTGAGGCTGGCCGGTCCTTTC
  TGGATGGCAGCCTGGAAGATGCCTTCAATGGACTCTTCCTTGCATTAGACCCACACAAGAAGCAGTACAA
  AGAGTTCCAGGATCTGAACCAAGAAGTCACTCACTTGGATGATGTTCTCAAATGCAAGCCGGCTGGCAGC
  CGCAGCAGGTCTTCCAGCCTAAGTCTGACGGTTGAGAGTGCTTTAGAAAGCTTTGATTTCCTCAACACCT
  CTGACTTTGACGAAGAGGAAGAGGATGGCGATGACGTCTGCCACGTTGGAGGAGGCGCAGACTCCGTGTT
  TTCAGATACTGAGACTGAGAAGAGTGGTTACAGGTCAGTTCACCCGGAAGCCAGGGGTCACCTTAGCGAG
  GCTCTGACTGAAGACACAGGTGTAGGCACCAGTGTGGCAGGAAGCCCGCTCCCACTCACCACAGGAAATG
  AGAGCCTGGACATCACCATAGTCAAACATCTACAATACTGCACCCAGCTCATCCAGCAAATCGTCTTCTC
  TAGCAAAACCCCCTTTGTGGCAAGAAGTCTCTTGGAGAAGCTGTCGAGGCAGGTCCTTGTGATGCAGAAG
  CTGGCAGCCGTGAGTGACGAGAACCTCGGGAACATCACCTCTGTGGTGGAAGCCATACCAGAATTTCACA
  AAAAGCTCTCCTTGCTGGCCTTCTGGACCAAATGCTGCAGCCCCAGCGGGGTCTACCACAGCTCCGCTGC
  CCGCCTCATCAAGCAGCTGGAGGCCAGCTTCGCCAGAAGCATCAACAAAGACTATCCAGGACTGGCAGAG
  CCAGTGTTTAGAACCCTGGTGTCCCAAATCCTGGACCGAGCTGAACCTCTCCTTTCCTCCAGCCTCTCGT
  CGGAAGTCATCACTGTCTTCCAGTACTACAGCTTCTTCACCAGCCACGGTGTGAGTGACCTGGAGACTTA
  CCTGGGCCAGCTAACCAGGCAAGTTGCCATGGTTCAGACTCTGCAGTCACTGAGAGATGAGAAACTGCTG
  CAGACTATGAGCGACCTTGCACCCAGCAACCTCCCCGCTCAACAGGAAGTCCTCAGGACGCTGGCCCTGC
  TGCTCACCAGGGATGACAATGAGGTCAGCGAAGCTGTGACACTCTACTTGGCAGCAGCCTCCAAAAATGA
  GCACTTCAGAGAAAAGGCCTTGCTCTATTATTGCGAAGCACTAACGAAGGCCAATCTCCAGCTACAGAAG
  GCAGCATGTCTGGCCCTAAAGAGCCTGGAGGCCACCGAAAGCATTAAAATGCTAGTGACGTTGTGTCAGT
  CTGACACTGAAGAAATCAGAACCGTGGCCTCGGAAACCCTCCTCTCTCTTGGAGAAGACGGGCGACTGGC
  GTACGAGCAATTAGACAAATTCCCTCGAGATTGTGTTAAAGTGGGAGGTCGTCATGGAACCGAAGTTGCC
  ACAGCCTTTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001286100
• Insert Size: 3162 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286100.1, NP_001273029.1
• RefSeq Size: 5466 bp
• RefSeq ORF: 3162 bp
• Locus ID: 193385
• UniProt ID: Q80U16
• Cytogenetics: 13 A3.1
• Gene Summary: Acts as an inhibitor of the small GTPase RHOA and plays several roles in the regulation of myoblast and hair cell differentiation, lymphocyte T proliferation and neutrophil polarization (PubMed:25588844, PubMed:27269051). Plays a role in fetal mononuclear myoblast differentiation by promoting filopodia and myotube formation (PubMed:17150207). Maintains naive T lymphocytes in a quiescent state and prevents chemokine-induced T lymphocyte responses, such as cell adhesion, polarization and migration (By similarity). Involved also in the regulation of neutrophil polarization, chemotaxis and adhesion (PubMed:25588844). Required for normal development of inner and outer hair cell stereocilia within the cochlea of the inner ear (PubMed:27269051). Plays a role for maintaining the structural organization of the basal domain of stereocilia (PubMed:27269051). Involved in mechanosensory hair cell function (PubMed:27269051). Required for normal hearing (PubMed:27269051).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) uses an alternate 5'-terminal exon and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (4) has a shorter N-terminus, compared to isoform 1.