Tlr7 (NM_001290756) Mouse Untagged Clone

CAT#: MC229313

Tlr7 (untagged) - Mouse toll-like receptor 7 (Tlr7), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tlr7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229313 representing NM_001290756
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGTTTTCGATGTGGACACGGAAGAGACAAATTTTGATCTTTTTAAATATGCTCTTAGTTTCTAGAG
  TCTTTGGGTTTCGATGGTTTCCTAAAACTCTACCTTGTGAAGTTAAAGTAAATATCCCAGAGGCCCATGT
  GATCGTGGACTGCACAGACAAGCATTTGACAGAAATCCCTGAGGGCATTCCCACTAACACCACCAATCTT
  ACCCTTACCATCAACCACATACCAAGCATCTCTCCAGATTCCTTCCGTAGGCTGAACCATCTGGAAGAAA
  TCGATTTAAGATGCAATTGTGTACCTGTTCTACTGGGGTCCAAAGCCAATGTGTGTACCAAGAGGCTGCA
  GATTAGACCTGGAAGCTTTAGTGGACTCTCTGACTTAAAAGCCCTTTACCTGGATGGAAACCAACTTCTG
  GAGATACCACAGGATCTGCCATCCAGCTTACATCTTCTGAGCCTTGAGGCTAACAACATCTTCTCCATCA
  CGAAGGAGAATCTAACAGAACTGGTCAACATTGAAACACTCTACCTGGGTCAAAACTGTTATTATCGAAA
  TCCTTGCAATGTTTCCTATTCTATTGAAAAAGATGCTTTCCTAGTTATGAGAAATTTGAAGGTTCTCTCA
  CTAAAAGATAACAATGTCACAGCTGTCCCCACCACTTTGCCACCTAATTTACTAGAGCTCTATCTTTATA
  ACAATATCATTAAGAAAATCCAAGAAAATGATTTTAATAACCTCAATGAGTTGCAAGTTCTTGACCTAAG
  TGGAAATTGCCCTCGATGTTATAATGTCCCATATCCGTGTACACCGTGTGAAAATAATTCCCCCTTACAG
  ATCCATGACAATGCTTTCAATTCATTGACAGAATTAAAAGTTTTACGTTTACACAGTAATTCTCTTCAGC
  ATGTGCCCCCAACATGGTTTAAAAACATGAGAAACCTCCAGGAACTAGACCTCTCCCAAAACTACTTGGC
  CAGAGAAATTGAGGAGGCCAAATTTTTGCATTTTCTTCCCAACCTTGTTGAGTTGGATTTTTCTTTCAAT
  TATGAGCTGCAGGTCTACCATGCATCTATAACTTTACCACATTCACTCTCTTCATTGGAAAACTTGAAAA
  TTCTGCGTGTCAAGGGGTATGTCTTTAAAGAGCTGAAAAACTCCAGTCTTTCTGTATTGCACAAGCTTCC
  CAGGCTGGAAGTTCTTGACCTTGGCACTAACTTCATAAAAATTGCTGACCTCAACATATTCAAACATTTT
  GAAAACCTCAAACTCATAGACCTTTCAGTGAATAAGATATCTCCTTCAGAAGAGTCAAGAGAAGTTGGCT
  TTTGTCCTAATGCTCAAACTTCTGTAGACCGTCATGGGCCCCAGGTCCTTGAGGCCTTACACTATTTCCG
  ATACGATGAATATGCACGGAGCTGCAGGTTCAAAAACAAAGAGCCACCTTCTTTCTTGCCTTTGAATGCA
  GACTGCCACATATATGGGCAGACCTTAGACTTAAGTAGAAATAACATATTTTTTATTAAACCTTCTGATT
  TTCAGCATCTTTCATTCCTCAAATGCCTCAACTTATCAGGAAACACCATTGGCCAAACTCTTAATGGCAG
  TGAACTCTGGCCGTTGAGAGAGTTGCGGTACTTAGACTTCTCCAACAACCGGCTTGATTTACTCTACTCA
  ACAGCCTTTGAAGAGCTCCAGAGTCTTGAAGTTCTGGATCTAAGTAGTAACAGCCACTATTTTCAAGCAG
  AAGGAATTACTCACATGCTAAACTTTACCAAGAAATTACGGCTTCTGGACAAACTCATGATGAATGATAA
  TGACATCTCTACTTCGGCCAGCAGGACCATGGAAAGTGACTCTCTTCGAATTCTGGAGTTCAGAGGCAAC
  CATTTAGATGTTCTATGGAGAGCCGGTGATAACAGATACTTGGACTTCTTCAAGAATTTGTTCAATTTAG
  AGGTATTAGATATCTCCAGAAATTCCCTGAATTCCTTGCCTCCTGAGGTTTTTGAGGGTATGCCGCCAAA
  TCTAAAGAATCTCTCCTTGGCCAAAAATGGGCTCAAATCTTTCTTTTGGGACAGACTCCAGTTACTGAAG
  CATTTGGAAATTTTGGACCTCAGCCATAACCAGCTGACAAAAGTACCTGAGAGATTGGCCAACTGTTCCA
  AAAGTCTCACAACACTGATTCTTAAGCATAATCAAATCAGGCAATTGACAAAATATTTTCTAGAAGATGC
  TTTGCAATTGCGCTATCTAGACATCAGTTCAAATAAAATCCAGGTCATTCAGAAGACTAGCTTCCCAGAA
  AATGTCCTCAACAATCTGGAGATGTTGGTTTTACATCACAATCGCTTTCTTTGCAACTGTGATGCTGTGT
  GGTTTGTCTGGTGGGTTAACCATACAGATGTTACTATTCCATACCTGGCCACTGATGTGACTTGTGTAGG
  TCCAGGAGCACACAAAGGTCAAAGTGTCATATCCCTTGATCTGTATACGTGTGAGTTAGATCTCACAAAC
  CTGATTCTGTTCTCAGTTTCCATATCATCAGTCCTCTTTCTTATGGTAGTTATGACAACAAGTCACCTCT
  TTTTCTGGGATATGTGGTACATTTATTATTTTTGGAAAGCAAAGATAAAGGGGTATCAGCATCTGCAATC
  CATGGAGTCTTGTTATGATGCTTTTATTGTGTATGACACTAAAAACTCAGCTGTGACAGAATGGGTTTTG
  CAGGAGCTGGTGGCAAAATTGGAAGATCCAAGAGAAAAACACTTCAATTTGTGTCTAGAAGAAAGAGACT
  GGCTACCAGGACAGCCAGTTCTAGAAAACCTTTCCCAGAGCATACAGCTCAGCAAAAAGACAGTGTTTGT
  GATGACACAGAAATATGCTAAGACTGAGAGTTTTAAGATGGCATTTTATTTGTCTCATCAGAGGCTCCTG
  GATGAAAAAGTGGATGTGATTATCTTGATATTCTTGGAAAAGCCTCTTCAGAAGTCTAAGTTTCTTCAGC
  TCAGGAAGAGACTCTGCAGGAGCTCTGTCCTTGAGTGGCCTGCAAATCCACAGGCTCACCCATACTTCTG
  GCAGTGCCTGAAAAATGCCCTGACCACAGACAATCATGTGGCTTATAGTCAAATGTTCAAGGAAACAGTC
  TAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290756
• Insert Size: 3153 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290756.1, NP_001277685.1
• RefSeq Size: 3862 bp
• RefSeq ORF: 3153 bp
• Locus ID: 170743
• UniProt ID: P58681
• Cytogenetics: X F5
• Gene Summary: Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate exon in the 5' region, compared to variant 1. Variants 1, 2, 3, and 4 encode the same isoform (a).