St18 (NM_001244693) Mouse Untagged Clone

CAT#: MC229310

St18 (untagged) - Mouse suppression of tumorigenicity 18 (St18), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: St18
• Synonyms: AV348974; mKIAA0535; Myt3; NZF-3; Nzf3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229310 representing NM_001244693
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGCCGAGGTTGAAGATAAAACGTTGCATACTCTTTCAAAAGGAACTGAAGTTCCAATGGATTCTC
  TGATCCCAGAGCTCAGGGTTCCATATGATTGCTCTATGGCGAAGAAGAGGAGAGCTGAAGAGCAGGCATC
  GGGAGTTCCAATTAACAAGAGGAAATCTCTGCTAATGAAACCCCGGCACTACAGCCCAGACATGGGCTGC
  AAGGAAAGCCCAGACAACAGGAACGAGGATGATGGCCTCCTAGAAACAAACGACCATGCCACTGCAGATG
  AAATCATGGTAAAATCCATGGATGAAACTCTTCATTTACCTGCACAAGACAGCTCACTCCAGAAGAAAGA
  CCAATACACATGTTATCCAGAGCTCATGGTCAAATCTCTGGTGCACTTAGGCAAATTTGAGGAAAGTGAG
  TCTGTGCAGACCACATGTGAGAACTTAAATGGCAGCAGTATCCAGTCTTTAAAAGCAGAGAGTGATGAAG
  CACATGAAGGTTCTATGGTTCATTCCGACAATGGAAGAGACAAAGTCCACCATTCCCAGCCACCTTTCTG
  CTCTTCTGGTGACAGCGAAAGTGACTCTGACAGTGCAGAGAATGGGTGGGGCAATGGCTCCAACTCATCA
  GAAGACACTGACACTCACAAAGGCCCCAAACACAAGCTGACATACAATAGAAAGGACCTGTTGGAAGTTC
  CTGAGATAAAAGCTGAAGATGACAAGTTTATCCCTTGTGAGAACAGGTGTGATTCTGACACAGATGGGAG
  AGACCCACAGAACTCTCATATGGAACCCTTGGTTGTGAAAGCCCAGCCTTCCTTCCCAGAGGTTGAAGAG
  GGTGAGAGCCTGGCCACAGTAACAGAAGAGCCTGCTGAGGTGGAGAAAGCAAAGGGGAACCTGAGTTTGC
  TGGAACAGGCTATTGCTCTACAGGCTGAACGAGGTTCTGTCTTCCATCACACTTATAAGGAGCTGGACCG
  ATTTTTCCTGGACCACCTGGCAAGGGAACGGAGGCAACCTAGAGTTACTGATGCCAATGGAAGACAAATC
  TTTACTAATAAACATTCACCAAGACCTGAAAGGAGGGAGGCCAAGTGCCCCATTCCTGGCTGTGATGGCA
  CGGGGCACGTAACAGGGCTCTACCCTCACCACCGCAGCCTTTCTGGGTGCCCCCACAAAGTGCGAGTTCC
  TCTGGAAATTCTCGCCATGCATGAGAACGTGCTCAAGTGTCCCACGCCAGGATGCACAGGAAGGGGACAT
  GTGAACAGCAATCGCAACACTCACAGAAGTCTTTCTGGTTGTCCGATTGCTGCAGCTGAAAAGCTGGCAA
  TGACCCAGGACAAAAGTCAACTTGATTCTTCTCAGACGGGGCAGTGTCCTGAGCAGGCACACAGGGTGAA
  TTTGGTGAAGCAAATTGAATTTAATTTCCGCTCACATGCCATCACTTCTCCAAGAGCCTCTGCATCAAAA
  GAACAAGAGAAGTTTGGGAAAGTACCGTTTGATTATGCCAGTTTTGATGCACAAGTATTTGGGAAACGTC
  CTCTCCTACAAACAGGGCAAGGACAAAAAGCACCACCATTTCCTGAATCAAAGCATTTTTCAAATCCAGT
  GAAATTTCCTAATGGACTGCCTAGTGCTGGCGCCCACACACAGAGCACAGTCCGTGCCAGCTCTTATGGC
  CATGGTCAGTACAGTGAAGACACCCACATAGCAGCAGCTGCTGCCATCCTGAACCTTTCCACCCGCTGCA
  GGGAAGCCACAGACATCCTCTCCAACAAACCACAAAGCCTGCGTGCCAAGGGAGCTGAGATAGAAGTAGA
  TGAAAACGGCACATTGGACTTAAGCATGAAAAAAAATCGAATCCACGACAAGTCTATACCCCCAACTTCC
  TCACCTACTACAATTACAACCCCATCCTCATCCCCATTCAACGCAAGCAGCCTTCTGGTCAATGCTGCCT
  TCTATCAGGCCCTCTCTGATCAAGAAGGCTGGAATGTGCCCATCAACTATAGCAAATCCCATGGGAAGAC
  AGAGGAGGAGAAAGAGAAAGATCCTGTGAACTTCCTAGAAAATTTAGAGGAAAAAAAGTTTGCTGGAGAG
  GCCTCTATCCCAAGCCCAAAGCCCAAGCTGCATACAAGAGATCTCAAGAAAGAATTAATCACCTGTCCAA
  CACCAGGATGTGATGGAAGCGGCCATGTCACAGGCAACTATGCATCTCACCGCAGTGTCTCTGGATGTCC
  TCTAGCAGATAAGACTCTGAAGTCTCTCATGGCTGCCAACTCTCAAGAGCTTAAGTGTCCAACCCCAGGT
  TGTGATGGTTCTGGTCATGTGACTGGAAACTATGCCTCCCACCGAAGCTTGTCTGGTTGCCCTCGTGCAA
  GGAAAGGTGGCATCAAAATGACCCCAACAAAAGAAGAAAAAGAAGACTCTGAACTTAGATGCCCTGTAAT
  AGGGTGTGACGGCCAAGGCCACATATCAGGTAAATACACATCACACCGCACAGCTTCTGGCTGTCCTCTG
  GCTGCCAAAAGACAGAAAGAGAATCCCCTCAATGGGGCACCTCTCTCCTGGAAACTGAACAAGCAAGAGC
  TTCCTCACTGTCCTCTGCCAGGATGCAATGGTCTGGGTCATGTAAACAACGTTTTTGTCACCCACAGAAG
  CTTATCTGGATGCCCTCTTAATGCACAAGCTATCAAAAAAGTCAAGGTCTCTGAGGAACTAATGACTATC
  AAGCTCAAAGCAACTGGGGGTATTGATGGTGATGAAGAAATTAGGCATCTGGATGAAGAAATCAAGGAAC
  TGAATGAATCCAACCTTAAAATTGAAGCAGATATGATGAAACTTCAGACCCAGATAACATCTATGGAGAG
  CAACTTGAAGACCATAGAGGAGGAGAACAAGCTTATAGAACAGAGCAATGAGAGTCTGCTGAAGGAGCTG
  GCAGGGCTTAGCCAGGCTCTCATCTCCAGCCTTGCTGACATCCAACTTCCACAGATGGGGCCTATCAATG
  AGCAGAATTTTGAAGCATATGTAAATACTCTCACAGACATGTACAGCAATCTGGAACAGGACTATTCCCC
  AGAATGCAAAGCTCTACTGGAAAGCATCAAGCAGGCGGTGAAGGGCATCCATGTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001244693
• Insert Size: 3138 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001244693.1, NP_001231622.1
• RefSeq Size: 5835 bp
• RefSeq ORF: 3138 bp
• Locus ID: 240690
• UniProt ID: Q80TY4
• Cytogenetics: 1 A1
• Gene Summary: Repressor that binds to DNA sequences containing a bipartite element consisting of a direct repeat of the sequence 5'-AAAGTTT-3' separated by 2-9 nucleotides. Represses basal transcription activity from target promoters (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate exon in the 5' UTR, compared to variant 1. Variants 1, 2, and 3 encode the same protein.