Ints3 (NM_145540) Mouse Untagged Clone

CAT#: MC229305

Ints3 (untagged) - Mouse integrator complex subunit 3 (Ints3), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ints3
• Synonyms: C77668
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229305 representing NM_145540
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTTACAGAAGGGAAAGGGGACCGTAGCAGCTGCTGCATCAGGAGCAGCGGGAGGTGGAGGAGGAG
  GAGCGGGAGCAGGAGCCCCAGGAGGGGGGAGGCTGCTACTTTCAACCAGTTTGGATGCCAAGGATGAGTT
  AGAGGAGAGGTTGGAAAGGTGCATGAGTATTGTCACATCGATGACCGCTGGTGTCTCCGAGAGAGAGGCC
  AATGATGCTCTCAATGCCTATGTGTGCAAAGGACCTCCCCAGCATGAGGAGATCTGCCTAGGCCTGTTTA
  CTCTTGTCCTCACTGAGCCTGCCCAAGCCCAGAAGTGTTACCGGGACTTGGCTCTGGTGAGTCGTGATGG
  TATGAACATCGTCCTGAACAAGATCAACCAGCTACTTATGGAGAAGTACTTGAAGCTGCAAGATACCTGC
  CGTACTCAGTTGGTGTGGTTGGTTCGGGAACTAGTAAAGAGTGGAGTTCTGGGAGCTGATGGCGTTTGCA
  TGACATTTATGAAGCAGATTGCAGGTGGCGATGTTACAGCCAAAAATATCTGGTTGGCAGAAAGTGTCCT
  GGATATCCTGACGGAGCAGAGGGAGTGGGTGCTGAAGAGCAGCATCCTCATTGCCATGGCTGTGTACACG
  TACCTCCGACTTATTGTGGACCACCATGGGACTGCCCAGCTCCAGACCCTTCGGCAGAAGGAAGTTGACT
  TCTGCATCTCCTTGCTTCGGGAACGGTTCATGGAATGTTTGATGATTGGCCGGGACCTTGTAAGATTACT
  TCAGAATGTTGCTAGGATACCAGAATTTGAACTACTGTGGAAAGATATCATCCATAACCCTCAGGCCTTG
  AGTCCTCAGTTTACAGGTATCCTACAGCTACTTCAGTCAAGAACATCCCGAAAATTTCTAGCCTGTCGTC
  TAACCCCAGACATGGAAACGAAGCTCCTCTTCATGACATCCCGGGTGCGGTTTGGCCAGCAAAAGCGGTA
  CCAGGACTGGTTCCAGCGCCAGTACCTCTCAACCCCTGACAGTCAGTCTCTGCGCTGTGACCTCATTCGA
  TACATCTGTGGGGTAGTCCACCCCTCTAACGAAGTGCTGAGTTCTGATATCTTGCCCCGGTGGGCCATCA
  TTGGCTGGCTCCTGACAACATGCACGTCCAACGTTGCTGCCTCCAATGCCAAGCTGGCTTTGTTTTATGA
  CTGGCTGTTCTTCAGCCCAGAAAAGGATAGTATTATGAACATAGAGCCAGCTATCCTGGTCATGCATCAC
  TCCATGAAGCCCCACCCAGCCATCACTGCCACGCTCCTGGACTTCATGTGCCGAATCATTCCTAACTTCT
  ACCCACCATTGGAAGGCCACGTTCGGCAGGGTGTCTTTTCTTCCCTCAACCACATTGTGGAGAAACGAGT
  CTTGGCACACTTGGCTCCCCTGTTTGACAACCCTAAATTGGATAAAGAGCTTCGGTCCATGCTTCGGGAG
  AAGTTTCCTGAGTTCTGCAGCTCGCCCTCCCCTCCTGTGGAAGTCAAAATTGAAGAGCCTGTTTCCATGG
  AAATGGACAACCACCTCTCAGATAAGGATGAGAGTTGCTATGACAATGCAGAGGCAGCCTTCAGTGATGA
  TGAGGAGGATCTTAATAGCAAAGGGAAGAAGAGAGAGTTTCGCTTCCACCCCATCAAGGAGACTGTCGTG
  GAGGAGCCGGTTGATGTCACCCCTTACCTTGATCAGTTGGACGAGTCCCTGAGGGACAAAGTGCTACAGC
  TACAGAAGGGAAGTGATACAGAGGCCCAGTGTGAGGTTATGCAGGAAATTGTGGATCAGGTCCTGGAGGA
  AGACTTTGACTCGGAGCAACTGTCTGTCCTGGCTTCCTGCCTACAGGAGCTCTTCAAGGCCCACTTTCGA
  GGGGAGGTGTTGCCTGAAGAGGTCACTGAAGAGTCCCTGGAGGAGTCAGTAGGGAAACCACTGTACCTAA
  TATTTAGGAACCTGTGCCAGATGCAGGAGGACAACAGCAGCTTCTCGCTGCTTCTGGACCTTCTCTCCGA
  GCTGTATCAAAAGCAACCCAAGATCGGCTACCACCTGCTCTACTACTTGAGGGCAAGCAAAGCCGCCGCA
  GGGAAGATGAACCTGTATGAGTCGTTTGCCCAGGCTACCCAGCTGGGTGATCTGCACACCTGTCTAATGA
  TGGACATGAAGGCCTGCCAAGAGGATGATGTGCGACTGCTATGCCACCTCACACCTTCCATCTACACAGA
  GTTTCCAGATGAAACTTTGAGGAGTGGGGAGCTGCTGAACATGATTGTGGCTGTTATTGACTCTGCACAG
  CTCCAGGAGCTGGTCTGCCATGTGATGATGGGGAACCTGGTCATGTTTCGGAAGGACTCAGTCCTCAACA
  TACTCATTCAGAGCCTAGACTGGGAAACGTTTGAGCAGTACTGTGCCTGGCAGCTCTTCCTGGCCCACAA
  CATCCCCTTGGAGACCATTATTCCTATTCTCCAGCACCTCAAATACAAGGAGCACCCAGAGGCCCTGTCC
  TGTCTCCTGCTTCAGCTACGGAGAGAGAAGCCGAGTGAGGAGATGGTGAAGATGGTGCTGAGCAGGCCCT
  GCCATCCTGATGACCAGTTCACCACCAGCATCCTGAGGCACTGGTGCATGAAGCACGACGAGCTGCTGGC
  AGAGCACATCAAGGCGCTGCTCATCAAGAACAACAGCCTGCCCCGCAAGAGGCAGAGCCTAAGAAGCTCT
  AGCAGCAAGCTGGCCCAGCTGACCCTGGAACAGATCCTGGAACACTTGGACAACCTAAGGCTCAACCTAG
  CCAACACCAAGCAGAACTTCTTTAGCCAGACACCAATCCTCCAGGCGCTGCAGCATGTCCAGGCAAGCTG
  TGACGAAGCTCACAAGATGAAGTTCAGTGACCTCTTCTCCCTGGCGGAGGAATATGAGGACTCTTCCACT
  AAGCCACCCAAGAGCCGGCGGAAGGCAGCTCTGTCCAGCCCTCGAAGTCGAAAGAATGCTACCCAGCCCC
  CCAATGCTGAAGAGGAGTCAGGCTCCAGCAGTGCCTCGGAGGAAGAAGATACAAAGCCTAAGCCTACCAA
  ACGAAAACGAAAAGGCTCCTCTGCTGTGGGTTCTGACAGTGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_145540
• Insert Size: 3126 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145540.3, NP_663515.2
• RefSeq Size: 4460 bp
• RefSeq ORF: 3126 bp
• Locus ID: 229543
• UniProt ID: Q7TPD0
• Cytogenetics: 3 F1
• Gene Summary: Component of the Integrator (INT) complex. The Integrator complex is involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Mediates recruitment of cytoplasmic dynein to the nuclear envelope, probably as component of the INT complex.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.