Atp9a (NM_001289445) Mouse Untagged Clone

CAT#: MC229290

Atp9a (untagged) - Mouse ATPase, class II, type 9A (Atp9a), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Atp9a
• Synonyms: IIa
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229290 representing NM_001289445
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTGAATGTCAGCCCAGGTGCTGTGAGTGGCTGAGATGTTGCGGTGGAGGGGAGCCCAGGCCCCGTA
  CTGTCTGGTTGGGACACCCCGAGAAGAGGGACCAGCGGTACCCTCGAAATGTCATCAACAACCAGAAGTA
  CAATTTCTTCACATTTCTTCCTGGGGTGTTGTTCAGCCAGTTCAGATACTTCTTCAACTTCTACTTCCTG
  CTTCTCGCCTGCTCGCAGTTCGTCCCAGAGATGAGGCTTGGCGCCCTGTACACCTACTGGGTTCCTCTGG
  GCTTCGTGCTGGCTGTCACCATCATCCGTGAGGCAGTAGAGGAGATCCGATGTTATGTGCGTGACAAGGA
  GATGAACTCCCAGGTCTACAGCCGGCTCACGTCACGAGGGACCGTGAAGGTGAAGAGTTCAAACATCCAG
  GTGGGAGACCTCATCCTTGTGGAAAAGAACCAGCGGGTCCCTGCTGACATGATCTTCCTGAGGACGTCAG
  AGAAAAACGGCTCTTGCTTCTTGCGCACGGATCAGCTGGATGGAGAGACAGACTGGAAGCTTCGGCTCCC
  GGTGGCCTGCACACAGAGGCTTCCCACGGCTGCTGACCTCCTGCAGATTCGGTCCTATGTGTACGCTGAA
  GAGCCCAACATCGACATTCACAACTTCCTGGGGACTTTCACCAGGGAAGACAGTGACCCTCCGATCAGTG
  AGAGTCTGAGCATTGAGAACACGCTGTGGGCCGGCACCGTCATAGCATCAGGCACTGTTGTAGGCGTTGT
  TCTCTACACTGGCAGAGAACTGCGGAGTGTCATGAATACTTCCGACCCCAGAAGTAAGATTGGCCTGTTC
  GACCTGGAGGTGAACTGCCTCACCAAGATCCTGTTTGGTGCGCTGGTGGTGGTGTCGCTGGTCATGGTGG
  CCCTGCAGCACTTTGCCGGCCGCTGGTACCTGCAGATCATCCGCTTCCTGCTCCTGTTTTCCAACATCAT
  TCCTATCAGCTTGCGTGTGAACTTGGACATGGGCAAGATCGTGTACAGCTGGGTGATCCGCAGGGATTCC
  AAAATCCCCGGGACCGTGGTTCGTTCCAGCACAATTCCTGAGCAGCTGGGCAGGATTTCGTACTTGCTCA
  CAGACAAGACAGGAACCCTGACCCAGAATGAGATGGTGTTCAAGCGGCTGCACCTGGGTACGGTGGCCTA
  CGGCCTGGACTCCATGGACGAAGTGCAGAGTCACATCTTCAGCATTTACACCCAGCAATCCCAGGATCCA
  CCTGCTCAGAAGGGCCCCACGGTCACCACCAAGGTTCGGAGGACCATGAGCAGCCGTGTCCACGAGGCTG
  TGAAGGCCATTGCACTCTGCCACAACGTGACACCCGTGTACGAGTCCAATGGTGTGACGGACCAGGCTGA
  GGCTGAGAAGCAGTTTGAGGACTCCTGCCGAGTGTACCAGGCATCCAGCCCGGATGAGGTGGCTCTGGTC
  CAGTGGACAGAAAGTGTGGGACTGACGCTGGTGGGTCGAGACCAGTCCTCCATGCAGCTGAGGACCCCTG
  GTGACCAGGTCCTGAATCTCACTATCCTTCAGGTCTTCCCGTTCACCTATGAGAGCAAGCGGATGGGCAT
  CATCGTGCGGGATGAGTCCACGGGGGAAATCACGTTCTACATGAAGGGAGCAGACGTCGTCATGGCTGGC
  ATTGTCCAGTACAACGACTGGCTGGAGGAGGAGTGTGGCAACATGGCCCGGGAGGGACTACGTGTGCTGG
  TGGTAGCCAAGAAGTCCCTCACAGAGGAGCAGTACCAAGACTTTGAAGCCCGCTACGTCCAGGCTAAGCT
  GAGTGTGCATGACCGCTCGCTGAAGGTGGCCACGGTGATCGAGAGCCTGGAGATGGAGATGGAGCTGCTG
  TGCCTGACTGGTGTGGAGGACCAGCTGCAGGCAGATGTCAGGCCCACGCTGGAGACGCTGCGCAACGCTG
  GCATCAAGGTTTGGATGCTAACAGGGGACAAGCTGGAGACAGCCACGTGCACAGCCAAGAACGCACATCT
  GGTGACCAGAAACCAAGATATCCATGTTTTCCGACTGGTGACCAACCGCGGGGAGGCCCACCTGGAGCTG
  AATGCCTTCCGTAGGAAGCATGACTGTGCCCTGGTCATCTCTGGAGACTCCCTGGAGGTTTGCCTCAAAT
  ACTATGAGTACGAGTTCATGGAACTGGCCTGCCAGTGCCCGGCTGTGGTGTGCTGCCGCTGTGCCCCAAC
  CCAGAAGGCCCAGATTGTTCGGCTGCTCCAGGAACGCACCGGGAAGCTCACCTGTGCAGTAGGGGACGGA
  GGCAATGACGTCAGCATGATCCAGGAATCCGACTGCGGCGTGGGCGTGGAGGGCAAGGAAGGGAAGCAGG
  CCTCGCTGGCAGCGGACTTCTCCATCACCCAGTTCAAGCATCTCGGCCGCTTGCTCATGGTGCACGGTCG
  GAACAGCTACAAGCGCTCGGCGGCCCTCAGTCAGTTTGTGATCCACAGGAGCCTCTGCATCAGCACCATG
  CAGGCTGTCTTCTCGTCTGTGTTCTACTTTGCATCCGTTCCTCTCTACCAAGGCTTCCTGATCATTGGGT
  ATTCTACCATCTACACGATGTTTCCCGTGTTCTCCCTGGTTTTGGACAAAGACGTGAAGTCGGAAGTCGC
  CATGTTGTATCCTGAGCTCTACAAGGACCTGCTTAAGGGGCGGCCACTGTCCTACAAGACGTTCTTAATT
  TGGGTGTTAATCAGCATCTATCAAGGGAGCACCATCATGTACGGGGCGCTGCTGCTGTTCGAGTCGGAGT
  TTGTACACATCGTGGCAATCTCCTTCACATCCCTCATCCTCACTGAGCTACTGATGGTGGCGCTCACCAT
  CCAGACGTGGCACTGGCTCATGACAGTGGCCGAGCTACTCAGCCTGGCCTGCTACATTGCCTCCCTGGTG
  TTCCTCCATGAGTTCATCGATGTCTACTTCATTGCCACCCTGTCATTCCTCTGGAAGGTGTCCGTCATCA
  CCTTGGTCAGCTGTCTCCCCCTCTATGTCCTCAAGTACCTGCGGAGACGGTTCTCCCCACCCAGCTACTC
  GAAGCTCACTTCCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289445
• Insert Size: 3096 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289445.1, NP_001276374.1
• RefSeq Size: 3739 bp
• RefSeq ORF: 3096 bp
• Locus ID: 11981
• Cytogenetics: 2 H3
• Gene Summary: Plays a role in regulating membrane trafficking of cargo proteins, namely endosome to plasma membrane recycling and endosome to trans-Golgi network retrograde transport. In complex with MON2 and DOP1B, regulates SNX3 retromer-mediated endosomal sorting of WLS, a transporter of Wnt morphogens in developing tissues. Participates in the formation of endosomal carriers that direct WLS trafficking back to Golgi, away from lysosomal degradation. Appears to be implicated in intercellular communication by negatively regulating the release of exosomes. The flippase activity towards membrane lipids and its role in membrane asymmetry remains to be proved.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) contains an alternate 5' terminal exon and initiates translation at an alternate start codon, compared to variant 1. It encodes isoform 2, which has a shorter and distinct N-terminus, compared to isoform 1.