Adamts13 (NM_001290465) Mouse Untagged Clone

CAT#: MC229287

Adamts13 (untagged) - Mouse a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 13 (Adamts13), transcript variant IAP-b

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Adamts13
• Synonyms: ADAM-TS13; ADAMTS-13; Gm710; vWF-CP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229287 representing NM_001290465
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCAGCTTTGCCTGTGGTTGACGTGCCAGCCTTGTTATGCTGTCAGTGTCAGAGGAATCCTCACTG
  GTGCCATCTTCATTCTGGGCTGCTGGGGGCTCTCTGACTTCCAGAAGAGTCTTCTTCAAGATCTGGAGCC
  CAAGGATGTGTCTTCTTACTTTGGCCACCATGCTGCTCCATTCACAGGCCATCCTCCCTCTCACCTCCAG
  AGACTGAGACGGAGAAGGACTTTGGAGGACATTCTGCACCTGGAACTCCTGGTAGCTGTGGGCCCCGATG
  TTTCCCGGGCTCATCAGGAGGACACAGAACGCTACGTGCTCACTAATCTCAATATCGGGTCAGAACTGTT
  GAGAAACCCATCCCTGGGAGTCCAGTTCCAGGTGCACCTGGTGAAGCTAATCACCCTCTCTGACTCAGAG
  AGTACTCCGAATATCACGGCCAACATCACCTCATCCTTGATGAGCGTCTGCGAGTGGAGCCAGACGATCA
  ACCCCCACGATGACAGGGATCCAAGTCACGCTGACCTGATTCTCTATATCACCAGGTTTGACCTGGAGTT
  GCCTGATGGCAACCAGCAGGTTCGGGGTGTCACCCAGCTGGGAGGTGCCTGCTCCCTTTCCTGGAGTTGC
  CTTATCACTGAGGATACTGGCTTTGACCTGGGGGTCACCATCGCCCATGAGATTGGGCACAGCTTCGGGC
  TGGACCATGATGGTGCTCCAGGTAGTGGCAGCACCTGCAAGGCCAGTGGCCACGTGATGGCGGCTGATGG
  CGCAACACCTACTGGAGGGACCCTGGAGTGGTCTGCCTGCAGCCAAAGGCAGTTGCAGCACCTACTCAGC
  ACAGGGCAAATGCACTGCTTCCAGGACCCACCTGGGCTGCAGTCAGGACTTACACGGCACCAGCTGATGG
  CACAGCCTGGCCTCTACTACAGTGCAGATGATCAGTGCCGTGTGGCTTTCGGTTCTGGGGCTGTCGCCTG
  CACCTTCTCCAGGGAGGGTCTGGATGTATGCCAGGCCCTGTCCTGCCACACAGACCCCTTGGACCAAAGC
  AGCTGCAGCCGCCTCCTTGTTCCTCTCCTGGATGGGACAGAATGTGGTGTGGAGAAGTGGTGCTCCAAGG
  CTCGCTGTCGCTCCCTAGCTGAGCTGGCTCCTGTGGCTGCAGTACATGGACACTGGTCTAGCTGGGGCCC
  CCATAGTCCCTGCTCCCGATCCTGTGGAGGAGGTGTGATTACCAGGAGGCGGTGGTGCAACAACCCCAGG
  CCTGCATTTGGGGGACGTGCATGTGTGGGTGAAGACCTCCAGGCTAAGATGTGCAACACGCAGGCTTGTG
  AGAAGACTCAGCTGGAGTTCATGTCCGAGCAGTGTGCCCAGACAGACAGACAACCACTGCAACTTTCCCA
  AGGCACTGCCTCCTTCTACCACTGGGATGCTGCTGTGCAGTATAGTCAAGGAGATACCCTGTGCAGACAC
  ATGTGCTGGGCTGTTGGAGAAAGCTTCATTGTCAGCCGTGGGGACAGGTTCCTAGATGGGACCCGTTGTG
  TGCCAAGTGGTCCTCAGGATGATGGGACCCTAAGCCTCTGTTTGTTGGGCAGCTGCAGGACCTTTGGCTG
  TGATGGCAGGATGGACTCCCAGAAGGTTTGGGATGCGTGCCAGGTGTGTGGAGGAGACAACAGCACCTGC
  AGCTCACGGAATGGTTCTTTCACAGCTGGGAGAGCCAGAGAATATGTCACGTTCCTGATTGTTACTCCCA
  ACATGACCAACGCACACATTGTCAACCGCAGGCCTCTCTTCACACACTTGGGTGAGATGCCAGAGGATCC
  TTCCTTGTACTTAGCCACTGATTTCTTAGTCTTCGAGATGCCTCCACCCCTAACACCTGGTGCCTGGTGG
  GAGAAACCCAACTCCCCAGACCCCCTTAGAGCTGCTACACTCACAGCTAAGCTTATCCCAGGGAAGGTGT
  ACAGACGATATGGAGGAGAATATGGGGATCTTACACACCCAGACATCACCTTTTCCTACTTTCAACTGAA
  GCAGCAGGCAGCCTGGGTATGGACCGCTAAGCGTGGACCCTGCTCAGTGAGCTGTGGGGCAGGGCTGCGC
  TGGGTGACCTACAGCTGCCAGGATCAAGCTCAAGACAAGTGGGTAAAGAACGCCCAGTGCCAAGGGAGCC
  CACAGCCACCTGCATGGCAAGAGCCTTGTGTCTCTGCCCCCTGCTCCCCATATTGGGTAGCTGGGGACTT
  CAGCCCATGTAGCGTGTCTTGTGGCGGGGGCCTTCGGGAGCGGTCACTGCGCTGTGTAGAGACCCAAGAT
  GGCTTCTTAAAGACACTGCCACCTGCCCGGTGCAGAGCAGTAGCCCAGCAGCCAGCAGCAGAAGTGGAAA
  ACTGCAACTCCCAGCCCTGTCCCACCAGGTGGGAGGTGTCAGACCCTGGCCCTTGCATGTCATCTGCCTG
  TGAGGCAGGTCTGGACTCAAGGAATGTGACATGTGTGTCCAGGGCGGGTGACCCGGAGAAGCCAGAAACT
  GCAGGCCCCTGCCGCACCGACGAGATGTCAGCTATGCTGGAGCCCTGCTCCAGGAGCCTGTGTTCTCCAG
  GCTTGGGTCAGGTGGACAACACCATGTCTCTGGGAGAGGAGGCTCCATCCCCGGTGGGCAGTGACAAGCC
  AGGGGCTCAGGCTGAGCATGTGTGGACCCCTCTGGTGGGGCTGTGCTCCATCTCTTGTGGGAGAGGTCTG
  AAGGAACTGTATTTCCTGTGCATGGATTCTGTCCTCAAAATGCCTGTCCAGGAAGAGCTATGCGGCTTGG
  CTAGTAAGCCCCCAAGCCGGTGGGAGGTCTGCAGGGCTCGCCCCTGTCCTGCTCGGTGGGAGACTCAAGT
  CTTGGCACCGTGCCCGGTGACCTGTGGTGGGGGGCGAGTGCCACTGTCTGTTCGTTGTGTGCAGCTAGAC
  CGTGGCCACCCGATATCTGTACCTCACTCCAAGTGCTCGCCAGTGCCTAAGCCAGGCTCCTTCGAGGACT
  GCAGCCCTGAGCCTTGTCCTGCTAGGGCACTAGTGTGGGAAGCCGCCCCCACATTCGCCGTCACAAGATG
  GCGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290465
• Insert Size: 3087 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290465.1, NP_001277394.1
• RefSeq Size: 3447 bp
• RefSeq ORF: 3087 bp
• Locus ID: 279028
• Cytogenetics: 2 A3
• Gene Summary: This gene encodes a member of "a disintegrin and metalloproteinase with thrombospondin motifs" (ADAMTS) family of multi-domain matrix-associated metalloendopeptidases that have diverse roles in tissue morphogenesis and pathophysiological remodeling, in inflammation and in vascular biology. In certain mouse strains (C57BL/6, for example) an intracisternal A-type particle (IAP) retrotransposon sequence is located in the intron 23 that causes an alternate splicing event resulting in a shorter transcript variants encoding shorter isoforms. The encoded preproprotein undergoes proteolytic processing to generate an active enzyme that cleaves von Willebrand factor (VWF) in circulating blood. [provided by RefSeq, Jul 2016]
  Transcript Variant: This variant (IAP-b) uses an alternate in-frame splice site and lacks an alternate in-frame exon in the central coding region, and it also lacks seven 3' exons but instead contains an alternate 3' terminal exon and thus differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (IAP-b) has a distinct C-terminus and is shorter than isoform 1. This isoform (IAP-b) may undergo proteolytic processing similar to isoform 1.