Lig3 (NM_001291247) Mouse Untagged Clone

CAT#: MC229270

Lig3 (untagged) - Mouse ligase III, DNA, ATP-dependent (Lig3), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lig3
• Synonyms: D11Wsu78e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229270 representing NM_001291247
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTTTGGCTTTCAAGATCCTCTTCCCGAGAAACCTTTGTGCCCTTGGCAGAAAAGAACTGTGCCTGT
  TCCCAGAACAGAATCGCTGGGCTGCCATAAGTCAGTTCAGCCAGTGGTCGGAAACAAATCTCCTCGGTGG
  GTGCTGCCTTCTCCAGAGAAGAAAGCCTGTCCTAGCTCTCCAGAGAGGTCATCTAAGACCACGTGCCACC
  CACCTTACTTTCTGGCCAGGGTCGCATGTGGGACTCTGTACTGGCCCCTGTGCGATGGCAGAGCAGCGGT
  TCTGTGTGGACTATGCCAAGCGGGGCACAGCTGGATGCAAGAAATGCAAGGAGAAGATTGTAAAGGGCGT
  GTGCCGCATTGGCAAAGTGGTGCCCAACCCCTTCTCAGAGTCTGGGGGTGATATGAAAGAGTGGTACCAC
  ATTAAGTGCATGTTTGAGAAACTGGAACGGGCTCGGGCCACCACAAAGAAAATTGAAGACCTCACAGAGC
  TGGAAGGCTGGGAAGAGCTGGAAGATAACGAAAAGGAACAGATAAGCCAGCATATTGCAGACCTGTCTTC
  TAAGGCAGCTGCCACACCTAAGAAGAAAGCTGCTGTCCAGGCTAAGCTGACAACCACTGGCCAGGTGACA
  TCTCCAGTGAAAGGTGCTTCGTTTATTACCAGTACCAATCCTCGGAAGTTCTCTGGCTTTTCAGCAAAAC
  CCAACAACTCTGAGCAAGCCCCCTCAAGCCCTGCTCCTGGAACGAGTCTGTCTGCAAGTAAATGTGACCC
  TAAGCACAAAGATTGTCTGCTACGAGAGTTCCGGAAGCTGTGCGCCATGGTGGCTGAAAATCCTAGCTAT
  AACACCAAGACCCAGATCATCCATGACTTCTTGCAGAAAGGCTCCACAGGAGATGGCTTTCACGGTGATG
  TGTACCTGACAGTTAAGCTGCTGCTGCCTGGTGTCATCAAGAGTGTTTACAACTTGAATGATAAGCAGAT
  TGTAAAACTTTTTAGCCGAATTTTTAACTGCAACCCAGATGATATGGCCCGGGACCTAGAACAGGGTGAC
  GTGTCAGAGACGATCAGAATCTTCTTTGAGCAGAGCAAGTCTTTCCCCCCAGCTGCCAAAAGCCTCCTCA
  CCATTCAGGAAGTGGATGCCTTCCTCCTGCACCTCTCCAAGCTCACCAAAGAGGATGAGCAGCAGCAGGC
  CCTGCAGGACATAGCCTCCCGGTGTACAGCCAATGACCTTAAGTGCATTATTCGGCTGATCAAACATGAT
  CTGAAGATGAACTCGGGTGCAAAACACGTGTTAGATGCCCTTGACCCCAATGCTTATGAAGCCTTCAAAG
  CATCGCGCAACCTGCAGGATGTGGTGGAGCGAGTCCTTCACAATGAGCAGGAGGTGGAGAAGGACCCAGG
  CCGGCGACGGGCTCTGAGGGTCCAGGCCTCACTGATGACGCCTGTGCAGCCCATGCTGGCTGAGGCCTGC
  AAATCCATTGAATATGCAATGAAGAAGTGTCCCAATGGCATGTTCTCTGAGATCAAGTACGATGGTGAGC
  GAGTCCAGGTGCATAAGAAGGGGGACCACTTCAGCTACTTCAGCCGCAGTCTCAAGCCTGTCCTGCCTCA
  CAAGGTGGCCCACTTCAAGGACTATATACCCAAAGCTTTTCCTGGGGGTCAAAGCATGATCTTGGATTCC
  GAGGTACTCCTGATTGACAACAACACAGGCAAACCACTGCCCTTTGGGACTCTGGGAGTGCACAAGAAAG
  CTGCTTTCCAGGATGCTAATGTCTGCCTGTTTGTTTTTGATTGTATCTACTTTAATGATGTCAGCTTGAT
  GGACAGGCCTCTCTGTGAGCGACGGAAGTTTCTTCATGACAACATGGTTGAAATCCGTAACCGGATCATG
  TTCTCAGAAATGAAGCAAGTCACAAAAGCTTCAGACCTGGCTGACATGATAAACCGGGTGATCCGAGAGG
  GCTTGGAAGGGTTGGTGCTAAAGGATGTGAAGGGTACATATGAGCCTGGGAAGCGGCACTGGCTGAAAGT
  TAAGAAAGATTATTTGAATGAGGGGGCCATGGCTGATACAGCTGATCTGGTGGTTCTTGGGGCCTTCTAT
  GGGCAAGGGAGCAAAGGCGGAATGATGTCCATCTTCCTCATGGGCTGCTATGACCCTGACAGCCAAAAGT
  GGTGCACTGTTACAAAATGTGCTGGAGGCCATGATGATGCCACACTTGCCCGCCTGCAGAAAGAGCTGGT
  TATGGTGAAGATCAGCAAGGATCCTAGTAAGATACCCAGCTGGCTGAAGATCAACAAGATCTATTATCCT
  GACTTTATCGTCCCAGACCCAAAGAAAGCTGCTGTGTGGGAGATCACAGGGGCAGAATTCTCCAGATCTG
  AGGCACACACTGCTGATGGCATCTCCATCCGATTTCCTCGATGCACCCGAATCCGGGACGACAAGGACTG
  GAAATCTGCCACTAACCTCCCCCAACTCAAGGAACTGTACCAGCTGTCCAAAGACAAGGCAGACTTTGCT
  GTGGTGGCTGGAGATGAGGCAAGCCCCACTACTGGAGGCAGCAGTGGCGAGAACGAGGGCACTGCTGGGT
  CTGCTGGGCCCTGCAAGGGCCCCCCCAGTAAGTCCTCTGCCAGTGCCAAGACCACCGAACAGAAGCTCAA
  TAGCCCCAGTAGCAGAGGTGGCATCAAACCGATTCCAAAGCATTCCCCCATGAAACCAGGAGAGAAGCTG
  GCTGTGAAGTCTTCTCCAGTCAAAGTGGGAATGAAGAGGAAAGCTACTGATGAGACCCCATGCCTGAAAA
  AGGTACTGTTGGATGTCTTCACTGGGGTGCGGCTCTACTTGCCACCTTCTACACCAGACTTCAAACGTCT
  CAAACGCTACTTTGTGGCATTCGACGGGGACCTGGTACAGGAATTTGACATGGGCTCAGCCACACATGTG
  CTAGGTAACAGGGAAAAGAACACTGATGCCCAGTTGGTCTCCTCAGAGTGGATTTGGGCATGTATCCGGA
  AACGGAGGCTGATAGCTCCCTGCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001291247
• Insert Size: 3036 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291247.1, NP_001278176.1
• RefSeq Size: 5985 bp
• RefSeq ORF: 3036 bp
• Locus ID: 16882
• Cytogenetics: 11 50.23 cM