Prob1 (NM_001270646) Mouse Untagged Clone

CAT#: MC229266

Prob1 (untagged) - Mouse proline rich basic protein 1 (Prob1)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Prob1
• Synonyms: Gm1614
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229266 representing NM_001270646
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGACTGCGCTCGCCCCGCTAGCCCTTCCTGGGCTCTCAAGGCGGCTGCCTGCCCCAGCGGGGCGTC
  AGGACTCGTCCGGTTCGTCCGGCTCCTACCACACAGCTCCGGGTTCTCCGGAGCCCCCGGACGTTGGGCC
  GGACACAGAAGGCCGGGGGACTTGGCTCTGGGTGGCTCCTGGGCGGGGGGCGGGCGCGCAGCCTGTCCTG
  TCCGTCAGCGCCCAGAATAGCCGCCAGCAGCACGGCTCCGGCTCGGGTTTCCCGCGAGGCCCGGGCTCCG
  GCCCGCTGCCGCCCCGGCCCCAGCTGCGCATGCTGCCGTCGGGGGAGATGGAAGTCATCTTCGGCGCGGG
  GCCTCTGTTCAACCGCTCCGACGCGGAGGATCTGCAGGAGCAACAGCTCATGGCGCCTACCTTCAGCAGT
  CCCCAGCTGCCCGGGCCAGCGTCTCCTGCCTCTGTCTCATCGCAGCCGCAGGTCCCCGACGGAGGCTCTC
  GCTGGGCCACCTACCTGGAGCTGCCGCCCCGCGAGCCGAGTCCTGCCGTCTCAGGCCAGTACGAGTGTGT
  GGAGGTGGCCCTGGAGGAGCGCAGCGCGCCCGTTAGACCCCGCACTGTGCCGAAACGTCAGATCGAGCTG
  CGTCCGCGGCCTCGGAGTCCCTCGCAGGACAGTCGCGCGCCGCGTCCCCGACTGCTCCTGCGCACGGGCT
  CCCTGGACGAGTCTCTGAGCCGCTTGCAGGCCGCCGCGGGCATCGTGCAGACGGCGCTGGCTAGAAAACT
  AGGCTCTGCGGTCCCAGCCCCCAGCAACGTCACTTTTAAGTCTACCGCCAAGCCAGAATCCACGACGAAC
  TCTCAGGAGACGACAGACAGCACTCGTGTGCTCCTGGAAGAGGCTAAGTCTCGGCCACCCCGCGCGCACG
  ATAATTCCTCCTCTGCCAGAGTCCCGCGGCCTTGGCCTAGCCTCCGAGAGCGAGCGATTCGGCGGGACAA
  GCCGGCGCCCGGGACCGAGCCTCTGGGTCCAGTTAGTTCCAGCATCTTTCTGCAGTCAGAGGAGAAACCT
  CATCAGGCTTACAAACAGGAATCCAAGACCCAGTTTCCCCGTGAGACTCCTGATAGAACCGTGCCGAGAG
  CACGGAGTCCTCCTTTTCATTCTAGAGGTTCCCGGGTGGTTCCCAGTAGGGTTGTGAGGCCCAGGAGCCC
  CTCTCCTCCGCAGCAGGCTCCAAACGGGTCTCCCCACAACCCCCCACAACCTTCTAAGATTGTTTGGAGA
  CGGAGCAGCCCGGCCTTCCCTGGCGGCACATCCTCTGCGTGGGAAAATCGGAATGCTTCCGTCGAGGAAG
  TCGTCAGCAGAAGGACCCCTTCCCCACCACCACTTCCCCAATGGACTCAGGGTGTCGCTAGAGTTGGGAA
  CCCATGTCCTGAAGCTCCTCTTGTGTGGAAGGTTCCGAATTCGAGAGGCGGAGATGCAGTAGGGCTGAGT
  AGGGACTCGTCGCTGCCAGCCCTGCTCCCGCGGGAAGCACCTCATCCTATTGAAACGCGTACCCCATCTC
  CCGGAGAGACTTGGAGTCCTGTTGTGCAGGGTGCATCGACGGCACTGCATCAGGAAGCTATGAACGGTCT
  AACCCAGGAGCTGGAGCCGCCTACACCATCAGCACCCGCGACTCCAGAGCTCACAGAGGCTCTGTGTCTG
  CTCTCACTGGAAAATGCGGGTATCGCCGTCAGAGGCGGTCAACGGTCTCCAGATGTGGATACACCGGAAC
  AGCCCCGAAGTCACCTCACAAGCACTCTGGACGACGATGTGTGCCCAGAAGCCGTGGGCTCGGGAGAAGC
  GGCTTCTGAACGCCCGCGCGTGACCATTCCGCGGCCGCGAGACGTGCGCAAGATGGTTAAGACTACTTAT
  GCACCAAGCTTCCCTGCAGGGACCCCAGGCTCCGGGCTTCCCGCGCCTCCTGCGGACACCTGCGGGGAGG
  ACGGTGATGCATCCAAAACTCACAAGCTCCCCGCACTTGAGTCGCCCGCCCCGGCTCATTACACTTCCAT
  TTTTCTCAAGGACTTTCTGCCTGTGGTACCTCATCCCTACGAGTCACCAGAGCCTTCACTCCATGCAACC
  CCTAAGGATGCCTCCCAGTCCAGCGGGGTCCCGAGGCGGCGGGCAGAGAACAGCACCGCGAAGCCTTTCG
  CTCGAAGTGAGATCCGCTTGCCTGGCGCTCTAGCTTTCGGCCAGCGGCGGGAGAAAACCCCGAGTGTCCA
  AAGACGCGGTCCTGGAGGAGAAAACCCGGATGCTGAGGCCCAGCGCCTGGTCCGGGACCCTGAGGGTCGG
  ACCAGCCCTTTAGGAGGCGCTCGCACCTCAACCCAATGTTCCCCTTTAGAGTCGACAGGGACGCGACCAC
  CTCGCCCCGGCTCCCCGCAGGCGTGTCCAAACTCGAGCCTTAGGGCAGCGCCGGAATTGGAGACACCGCT
  TGTGGCTCCTGCAACTGCGGTCCAGGCGCCCCTACTTCCCGATCATCCGGCGACGGCAGCCAGGGCCGCT
  GCACCCTTCCCGCGAGCTGCTTCTGCGCCTCCCACCGACCGGCTCCCCCCTGCATCTTCCCTGGGGCGGA
  GGCCTCCGGGAGCCGCCCCCGCGGGGAAGGTCCTTGTAGACCCGGAGAGCGGCCGGTACTACTTTGTGGA
  GGCGCCGAGGCAGCCGAGGCTGCGACTACTTTTCGATCCCGAGAGCGGGCAGTACGTGGAAGTACTGCTG
  CCACCCTCGCCCTCTCCCTCCCGGCAGCCACGCCAGCCCTATGCCCCACTGGCCCTAGGGCCCGGCCTCT
  ACCCGCCCGCCTATGGGCCTATCTCTGGTCCATCACTGTCGCCGCTGTCCCCCAACCTGTCGGCCCTTGG
  TAGTCTCCAGCTACCTTGGGCTCCTGAGGCTGGACCCCTGGAGGGAATGTATTACATGCCCTTGAGCGGG
  ACCCCTAGTCCCGCACCACCAATGCTCTTCTGTGCCCCACCCTCCAGTTCTGGTCCCATCCAGTCCAGCA
  AGGGTTCTGTGTTTCCTTTGTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001270646
• Insert Size: 3033 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001270646.1, NP_001257575.1
• RefSeq Size: 4849 bp
• RefSeq ORF: 3033 bp
• Locus ID: 381148
• Cytogenetics: 18 B2