Mllt10 (NM_001252561) Mouse Untagged Clone

CAT#: MC229254

Mllt10 (untagged) - Mouse myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila), translocated to, 10 (Mllt10), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mllt10
• Synonyms: Af10; B130021D15Rik; D630001B22Rik; mKIAA4140
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229254 representing NM_001252561
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAGATGTGAGCTGTGTCCCCATAAAGATGGAGCTTTAAAAAGAACAGATAATGGGGGTTGGGCTC
  ATGTGGTTTGTGCCCTCTATATTCCAGAGGTACAATTTGCCAATGTTTCTACAATGGAGCCAATCGTTTT
  ACAGTCTGTCCCACATGATCGTTATAATAAGACTTGCTATATATGTGATGAACAAGGACGAGAAAGCAAA
  GCAGCCACTGGTGCTTGCATGACATGTAATAAACATGGATGTCGACAGGCTTTCCATGTAACATGTGCTC
  AGTTTGCCGGGCTGCTTTGTGAAGAAGAAGGGAATGGCGCAGACAATGTTCAATACTGTGGCTACTGTAA
  ATACCACTTTAGTAAGCTGAAAAAGAGCAAACGGGGATCTAATAGGTCATATGAGCAAAGTTTAAGTGAC
  TCTTCCTCTCACTCTCAGGATAAGCATCATGAGAAAGAGAAAAAAAAATATAAAGAAAAGGACAAACACA
  AACAAAAACACAAGAAGCAACCAGAACCGTCACCTGCCTTGGTTCCTTCTTTGACTGTTACTACAGAAAA
  AACTTATACAAGCACTAGCAACAACTCCATATCTGGATCATTGAAGCGCTTGGAAGATACTGCAGCAAGA
  TTTACAAATGCAAATTTCCAGGAAGTCTCTGCCCATACCTCTAGTGGAAAAGATGTTTCAGAGGCTAGAG
  GGTCAGAGGGCAAAGGGAAGAAATCTTCAGCTCACAGCTCAGGTCAAAGGGGAAGGAAGCCTGGTGCAGG
  AAGAAATCCAGGAACAGCTGTGTCAGCATCTAGCCCCTTTCCACAAGGCAGTTTTTCAGGAACTCCAGGC
  AGTGTAAAGTCATCTTCTGGAAGTTCAGTGCAGTCTCCCCAGGACTTCTTAAGCTTTACAGACTCAGATC
  TGCGAAGTGACAGTTACACTCACACCCAGCAGCCATCATCAACCAAAGATGTACATAAAGGAGAGTCTGG
  AAGCCAGGAAGCGGCAGTAAACAGTTTTAGCTCTTTAGTTGGTCACCCTGTGACCTCGACTGTTATTTCA
  CAGCCTAAAAGCTTTGACAATTCACCTGGAGAGTTGGGTAGTTCGAGCCTCCCTACAGCAGGATATAAGC
  GGGCTCAGACTTCTGGTATAGAAGAAGAAGCTGTAAAGGAAAAGAAACGAAAAGGAAATAAGCAAAGCAA
  ACATGGCCCCGGCCGACCCAAAGGAAACAAAAATCAAGAAAATGTTTCTCACCTCTCAGTTTCTTCTGCT
  TCACCAACATCGTCCGTAGCATCAGCTGCAGGAAGTGTAACAAGCTCTAGTCTTCAGAAATCTCCTACGC
  TGCTCAGGAATGGAAGTTTGCAGAGTCTTAGTGTTGGCTCGTCTCCAGTTGGCTCAGAAATTTCCATGCA
  GTATCGACATGATGGAGCTTGTCCAACAACTACCTTCTCAGAGTTGCTGAATGCAATACACAATGGTATT
  TATAACAGCAATGATGTAGCCGTATCCTTTCCAAACGTGGTGTCTGGCTCAGGATCTAGTACTCCTGTCT
  CTAGCTCTCATATACCTCAGCAGTCATCTGGTCATTTGCAACAGGTGGGAGCTCTCTCCCCCTCAGCTGC
  GTCATCTGTAACCCCTGCTGCTGCTACAACTCAGGCAAATACCGTTTCTGGATCTTCTCTCAGTCAGGCA
  CCAGCCCATATGTATGGCAGTAGATTAAATCAGAATCCATCCATGGCAGTTCTCATAGCTCAGTCTGAAA
  GCAGCCAAACAGATCAAGATCTTGGAGACAATGCCCGTAGCCTGGGTGGCCGAGGAAGCTCGCCCCGAGG
  AAGCCTCTCTCCGAGATCCCCTGTGAGCAACTTACAGCTTCGCTATGATCAACCGAGCAATAGCAGTTTG
  GAAACTGTGCCTCCAGTAGCAGCAAGTATTGAACAGCTCTTGGAGAGGCAGTGGAGTGAAGGACAGCAGT
  TTTTACTAGAGCAGGGGACTCCTGGTGACATTCTAGGAATGTTAAAGTCATTACATCAACTTCAAGTTGA
  AAATCGAAGATTAGAAGAACAGATCAAAAATTTGACTGCCAAGAAAGAACGTCTTCAGTTGTTGAATGCA
  CAGCTTTCAGTGCCTTTCCCAGCCATAACGACAAACCCCAGTCCGTCCCATCAGATGCACACATACACAG
  CACAGACTGCTCCTCCTCCTGATTCCCTGAACAGCAGTAAGAGCCCACATATAGGAAACAGCTTTTTACC
  TGACAATTCTCTTCCTGTATTAAATCAGGACTTGACCTCCAGTGGACAAAGCACCAGCAGTTCTTCTGCT
  CTTTCTACTCCCCCTCCTGCTGGCCAGAGTCCTGCTCAGCAGAGCTCTGGAGTCAGTGGGGTTCAGCAGG
  TGAATGGCGTGACAGTGGGGGCACTAGCTAGTGGGATGCAGACCGTCACATCCACCATCCCAGCTGTGTC
  CGCAGTGGGTGGAATAATTGGAGCTCTGCCAGGTAACCAACTGGCAATTAACGGCATTGTGGGAGCTTTA
  AATGGTGTGATTCAGACTCCAGTCACAATCTCCCAGAACCCTGCCCCTCTCACCCACACCAGTGTACCAC
  CTAATGCAGCGCATCCAATGCCGGCCGCCGCACTGACCAACAGTGCCTCGGGACTAGGGCTGCTGTCGGA
  CCAGCAGAGACAGATGTTTATCCAGCAGCAGCAGTTCCAGCAGCTGTTAAACTCCCAGCAGCTCACACCC
  GAACAGCATCAGGCCTTCCTATACCAGCTGATGCAGCAGCAGCACCACCCGCCTGAGCTCCAGCAGCTGC
  AGCTCCCGGGACCCACGCAGATCCCCATCAACAATCTCCTTGCAGGTGCTCAGGCGCCTCCACTCCACAC
  AGCTACCACCAACCCCTTCCTCACCATCCACGGGGACAGCACGAGCCAGAAGGTCACAAGACTTAGTGAT
  AAAACTGGGCCTGTAGCTCAAGAGAAAAGTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001252561
• Insert Size: 2973 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252561.1, NP_001239490.1
• RefSeq Size: 5116 bp
• RefSeq ORF: 2973 bp
• Locus ID: 17354
• Cytogenetics: 2 A3
• Gene Summary: Probably involved in transcriptional regulation. Binds to cruciform DNA (By similarity). In cells, binding to unmodified histone H3 regulates DOT1L functions including histone H3 'Lys-79' dimethylation (H3K79me2) and gene activation (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) includes an alternate exon and uses an alternate start codon, compared to variant 1. The encoded isoform (2) is shorter and has a distinct N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.