Dlgap4 (NM_001277186) Mouse Untagged Clone

CAT#: MC229253

Dlgap4 (untagged) - Mouse discs, large homolog-associated protein 4 (Drosophila) (Dlgap4), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dlgap4
• Synonyms: AI225853; BC024558; DA; DAP-4; DAP4; S; SAPAP-4; Sapap4; WBP1; WBP16
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229253 representing NM_001277186
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGGCCTCGGTGACAGCCGCCCCCGCCACCTCTCCGACAGCCTGGACCCACCTCACGAGCCCCTCT
  TTGCTGGACCTGACCGAAACCCCTACCTGCTGTCGCCCACCGAGGCCTTTGCCCGGGAAGCCCGCTTCCC
  CGGGCAAAACACACTGCCGGGGGATGGGCTCTTCCCACTCAACAACCAGCTGCCCCCACCAAGCAGCACC
  TTCCCCCGAATCCACTACAACTCCCACTTTGAGGTGCCCGAGGAAAGCCCATTCCCCAGCCACGCCCAAG
  CCACTAAGATCAATCGGCTGCCCGCCAACCTCCTGGACCAGTTTGAGAAACAACTGCCTATCCACCGAGA
  TGGCTTCAGCACCCTCCAGTTCCCCCGGGGGGAGGCCAAGGCCAGGGGTGAGAGTCCAGGCCGCATTCGC
  CACCTGGTCCACTCTGTCCAGCGTCTCTTCTTCACTAAGGCACCCTCCATGGAGGGCACCGCGGGCAAGG
  TGGGTGGCAATGGCAGCAAGAAAGGTGGCCTGGAGGACGGAAAAGGCCGGAGGGCCAAAAGCAAGGAACG
  GGCCAAGGCTGGGGAGCCCAAACGGCGAAGCCGCTCCAACATCTCAGGCTGGTGGAGTTCAGATGACAAC
  TTGGACGGTGAGGGTGGTGCCTTCCGCAGTGGCCCTGCCTCGGGGCTGATGACTCTGGGCCGCCAGCAAG
  AACGCACCCAGCCCCGCTACTTCATGCACGCCTACAACACCATCAGTGGGCACATGCTCAAAACCACCAA
  GAACACTACCACCGAGCTGACTGCCCCGCCGCCCCCGCCTGCTCCCCCTGCTACCTGTCCCAGCCTTGGA
  GTGGGCACCGACACCAACTATGTCAAACGTGGTTCCTGGTCCACTCTGACCCTCAGTCATGCCCATGAGG
  TCTGCCAGAAGACCTCAGCCACTTTGGATAAGAGCCTGCTCAAGTCCAAATCCTGCCACCAGGGTTTGGC
  CTACCATTACCTGCAGGTGCCCGGCGGAGGGGGAGAGTGGAGCACCACGCTGCTATCCCCTCGAGACATG
  GATAGCACCGCTGAGGGGCCCATCCCGTGCCGGCGCATGCGCAGTGGCAGCTATATCAAGGCCATGGGCG
  ATGAAGACAGTGATGAGTCTGGGGGTGGTAGCCCCAAACCTTCACCCAAGACTGCAGCGCGGCGTCAGAG
  CTACCTGAGGGCTACGCAGCAGTCCCTGGGAGAGCAGAGCAACCCTCGACGGAGTTTGGACCGCTTGGAT
  TCAGTGGACATGCTGCTGCCTTCCAAGTGCCCCAGCTGGGAAGACGACTACAACCCCATCAGTGACAGCC
  TCAATGACTCCAGCTGTATCAGCCAGGTTTTTGGACAGGCTTCCCTGATCCCCCAGTTGTTTGGCCATGA
  TCAACAGGTCCGGGAGGCAGATCTGAGCGACCAGTACGAGGCAGCCTGCGAGTCGGCCTGCAGTGAGGCC
  GAGTCTACAACCGCAGAGGCACTCGACTTGCCGCTGCCCAGCTACTTCCGCTCACGCAGCCACAGCTACC
  TGCGAGCCATCCAGGCAGGCTGCTCGCAAGAGGAGGACAGTGTCTCCCTGCAGTCCCTCTCCCCACCGCC
  CAGCACTGGCAGCCTCAGCAACAGTCGCACGCTTCCAAGTTCATCATGCCTAGTGGCATATAAGAAGACC
  CCACCACCGGTCCCTCCACGAACCACATCGAAACCGTTCATCTCAGTCACCGTCCAGAGCAGTACTGAGT
  CTGCTCAGGATACCTACCTGGACAGTCAAGACCACAAGAGCGAAGTGACGAGCCAGTCGGGCTTGAGCAA
  CTCATCAGACAGCCTGGACAGCAGTACCCGGCCACCCAGTGTGACACGGGGTGGAATTACCCCAGGCCCT
  GAAGCTCCGGAGCCACCCCCTAAGCATGCAGCTCTGAAGAGTGAACAAGGGACACTGACGAGCTCTGAGT
  CCCATTCCGAGGCCATCCCCAAAAGGAAACTGTCATCGATAGGAATACAAGAGAGGACTAGAAGGAGCGG
  TTCCCACCTCTCGGAGGACAACGGACCAAAAGCGATCGATGTGATGGCGCCCTCCTCAGAAAGCAGTGCC
  CCCTCTCACAGCATGTCCTCCCGTCGGGACACTGACTCTGATACCCAGGATGCCAACGACTCCAGTTGTA
  AGTCATCTGAGAGGAGTCTCCCAGACTGTACCTCACACCCCAATTCCATCAGCATTGATGCTGGCCCCCG
  ACAGGCCCCCAAGATTGCCCAGATCAAGCGCAACCTCTCCTATGGAGACAACAGCGACCCTGCCCTGGAG
  GCGTCTTCGCTGCCCCCACCCGACCCTTGGCTGGAGACTTCCTCCAGCTCCCCAGCAGAGCCCGCTCAGC
  CAGGGGCCTGCCGCAGGGATGGCTACTGGTTCCTGAAGCTCCTTCAGGCAGAAACGGAGAGACTGGAAGG
  CTGGTGCTGCCAGATGGACAAGGAGACCAAAGAGAACAACCTCTCTGAAGAAGTCTTAGGGAAAGTTCTC
  AGTGCTGTGGGCAGCGCCCAGCTCCTGATGTCCCAGAAATTCCAGCAGTTCCGCGGCCTCTGTGAGCAGA
  ACTTGAACCCTGATGCCAACCCCCGTCCGACAGCCCAGGACCTGGCAGGGTTCTGGGACCTGCTGCAGCT
  GTCCATTGAGGACATCAGCATGAAGTTCGATGAGCTCTACCACCTCAAGGCCAACAGCTGGCAGCTGGTG
  GAGACCCCCGAGAAGAGGAAGGAAGAGAAGAAGCCACCCCCTCCAGTCCCGAAGAAGCCAGCCAAATCCA
  AGGCGGCAGTGAGCCGCGACAAGGCCTCAGACGCGGGGGACAAGCAGCGTCAGGAGGCCAGAAAGAGACT
  CCTGGCCGCCAAGCGAGCAGCGTCTGTGCGGCAGAACTCGGCCACAGAGAGTGCGGACAGCATCGAGATT
  TATGTCCCCGAGGCCCAGACCAGGCTCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001277186
• Insert Size: 2970 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001277186.1, NP_001264115.1
• RefSeq Size: 4866 bp
• RefSeq ORF: 2970 bp
• Locus ID: 228836
• UniProt ID: B1AZP2
• Cytogenetics: 2 H1
• Gene Summary: This gene encodes a membrane-associated guanylate kinase found at the postsynaptic density in neuronal cells. The encoded protein may play a role in synapse organization and neuronal signalling. Alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq, Mar 2013]
  Transcript Variant: This variant (4) lacks an exon and contains an alternate in-frame exon, compared to variant 1. The encoded isoform (d) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.