Epb41l2 (NM_001199265) Mouse Untagged Clone

CAT#: MC229250

Epb4.1l2 (untagged) - Mouse erythrocyte protein band 4.1-like 2 (Epb4.1l2), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Epb41l2
• Synonyms: 4.1G; AW555191; D10Ertd398e; Epb4.1l2; NBL2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229250 representing NM_001199265
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTACTGAAGTTGGCTCTGCATCTGAAGTGAAGAAGGGCTCTGACCAGGCTGGAGCAGATGCCTCCA
  AGGAGAAAGCTAAAGAAGTAGAAAATGAGCAGACTCCAGTATCTGAGCCAGAGGAAGAAAAGGGTTCCCA
  GCCAGGTCCTCCGGTTGAGAGGCAAAGCACTCCCCGCCTGCGGAAACGAGGGAAGGATCCGTCTGAGAAC
  AGGGGCATCTCTCGATTCATACCCCCGTGGCTTAAGAAGCAGAGGTCTTACAATTTAGTAGTAGCCAAAG
  ATGGAGGAGATAAAAAAGAGCCTACTCAAGCTGACGTGGAAGACCAGATCTTAGGCAAAGAGGAATCCCT
  TCCTGAAGAAGAGAGTCGGGCCAAGGGTGACGCAGAAGAAATGGCTCAGAGGAAGCACCTGGAGGTTCAG
  GTGGAGGTCAGGGAAGCAAAACCAGCCTTGAAAAGCAGCGTGGAGACCCAGCCTGCTGAAGAAGTGAGGA
  AGGACAAGGAGGAAACGATACAAGACACACAGGAAGAGAAACTGGAAGGAGGTGCAGCCAAAAGGGAGAC
  AAAGGAAGTACAGACAAGTGAGCTGAAGGCAGAGGTGGCCTCTCAGAAAGCTACCAAGAAGACCAAGACT
  GTCCTGGCCAAAGTGACCCTTCTAGATGGCACGGAATACAGCTGTGACCTTGAGAAACGTGCCAAGGGAC
  AAGTGTTGTTTGACCGAGTGTGCGAGCACCTGAACCTCTTGGAGAAGGACTACTTTGGACTTCTATTTCA
  GGATCATCCTGAGCAGAAAAACTGGCTAGATCCTGCAAAGGAAATAAAGAGACAGCTGAAAAATCTTCCC
  TGGCTGTTCACTTTCAATGTGAAATTTTATCCTCCAGATCCTTCTCAGCTGACTGAAGATATCACCAGAT
  ACTTCTTGTGCCTTCAGCTAAGGCAGGACATCGCCTCTGGCCGCCTGCCCTGCTCCTTTGTGACTCACGC
  CCTCTTGGGATCCTACACCTTGCAGGCCGAGCATGGGGACTATGACCCAGAAGAATATGACAGTATTGAT
  CTTGGTGACTTCCAGTTTGCCCCGGCCCACACCAAGGAGTTGGAGGAAAAGGTGTCAGAACTGCATAAAA
  CTCACAGGGGCTTATCTCCAGCACAAGCCGACTCTCAGTTCTTAGAGAACGCAAAGAGGCTTTCCATGTA
  CGGTGTTGACCTGCACCATGCCAAGGACTCTGAAGGTGTGGACATCAAGCTGGGTGTGTGTGCCAATGGC
  CTTCTCATTTACAAAGACAGACTAAGGATCAATCGTTTTGCTTGGCCGAAGATTTTGAAAATTTCCTATA
  AGCGCAGTAATTTCTACATTAAAGTTAGACCAGCAGAGCTGGAACAGTTTGAGAGTACCATTGGATTCAA
  ATTGCCAAACCACCGGGCAGCTAAACGGCTATGGAAGGTGTGTGTGGAACATCACACTTTCTACAGGCTT
  GTGTCTCCAGAGCAGCCACCAAAGACCAAGTTCTTGACCCTGGGGTCCAAATTCCGCTACAGCGGCCGCA
  CGCAGGCACAGACCAGAGAAGCAAGTACCCTCATAGACAGGCCGGCACCACAATTTGAGCGTGCCTCTAG
  TAAACGTGTCTCGAGGAGCCTAGACGGAGCCCCGATTGGTGTTGTGGACCAAAGCCCTCCTGGAGAGGGC
  TCAGTGCCTGGCCCTGGAGTTATCAGCTATACTACCATCCAGGATGGCCGGAGGGACAGCAAAAGCCCAA
  CAAAAGCTACACCCCTGCCCGCTGAGGGAAAGAAAAATACCTTGAGAGTAGACGGGGATAATATTTATGT
  CAGACATAGCAATTTAATGTTGGAGGACCTGGATAAGGCCCAGGAGGCCATTCTGAAACATCAGGCTAGC
  ATTAGTGAACTCAAGCGCAATTTCATGGCGTCCACACCTGAGCCTCGCCCCAGTGAATGGGAGAAGCGGC
  GGGTCACACCTCTGCCTTTCCAACCACAAGCGAGTTCACATGAGACTCTGAATGTAGTGGAGGAGAAGAA
  GCGGGCAGAGGTTGGGAAAGACGAAAGCGTAATCACAGAAGAAATGAATGGTAAAGAGATGTCACCGGGG
  CATGGTCCTGGGGAGACTCGTAAGGTGGAGCCTGTGGCACACAAAGACTCCACCTCCCTGTCTTCTGAGA
  GCAGCAGCAGCAGCAGTGAGAGTGAGGAGGACGTGGGAGAGTACCAGCCCCACCACCGAGTGACCGAGGG
  CACCATAAGGGAAGAGCAGGAGGAGTGTGATGAAGAGCTGGAGGAAGAGCCCGGCCAAGGAGCCAAGGTA
  GTAGAGAGGGAGGCAGCAGTGCCCGACGCCGTCCCAGACAGACAAGCAGGGGCCAGTGTGCTCCCAGTAG
  AAACAGAGGCCCAGGAACATGTAGTTGCCCAAAAGTTACCTGGAGAAAAGGGTGCACACGGAGGCACTGC
  TGAGCAGGACCCGAGAGAAGAAGCAGAGGAAGACCCGCACAGAGTTAATGGAGAGGTGCCCCATCTTGAC
  CTTGATGGTTTGCCAGAGATTATTTGTTGTTCAGAGCCACCAGTGGTAAAAACAGAGATGGTAACAATTT
  CTGATGCCTCACAAAGGACAGAAATCTCCACCAAGGAAGTCCCCATTGTTCAAACTGAGACCAAAACCAT
  CACATATGAGTCTCCACAGATTGACGGCGGGGCTGGTGGTGATTCGGGCGTGTTACTGACCGCACAAACC
  ATCACATCTGAGTCCGCGTCAACAACGACAACCACACACATCACCAAGACTGTGAAAGGTGGGATCTCTG
  AAACACGAATTGAGAAACGCATTGTGATCACAGGAGACGCAGCTCTGGATCATGATCAGGCGCTGGCTCA
  GGCCATACGGGAAGCGAGAGAGCAGCACCCTGATATGTCGGTCACAAGGGTGGTAGTGCACAAAGAAACA
  GAGTTGGCGGAGGAAGGAGAAGAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001199265
• Insert Size: 2967 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001199265.1, NP_001186194.1
• RefSeq Size: 4389 bp
• RefSeq ORF: 2967 bp
• Locus ID: 13822
• UniProt ID: O70318
• Cytogenetics: 10 12.26 cM
• Gene Summary: Required for dynein-dynactin complex and NUMA1 recruitment at the mitotic cell cortex during anaphase.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 both encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.