Nup98 (NM_001287167) Mouse Untagged Clone

CAT#: MC229248

Nup98 (untagged) - Mouse nucleoporin 98 (Nup98), transcript variant 5

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nup98
• Synonyms: 4732457F17; AI849286; Nup96
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229248 representing NM_001287167
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTAACAAATCATTTGGAACCCCCTTTGGGGGTAGTACAGGGGGCTTTGGCACAACGTCAACATTTG
  GGCAAAATACTGGCTTTGGTACGACTAGTGGAGGAGCATTTGGAACATCTGCATTTGGTTCTAGCAACAA
  TACTGGAGGCTTATTTGGAAATTCACAGACCAAACCAGGAGGATTATTTGGTACCAGTTCATTTAGCCAG
  CCAGCAACCTCCACAAGCACTGGGTTTGGGTTTGGCACATCAACAGGAACATCAAATAGCTTATTTGGAA
  CTGCAAGTACCGGGACCAGTCTTTTCTCATCCCAGAACAATGCATTTGCACAAAATAAACCAACTGGCTT
  TGGGAATTTTGGAACCAGTACTAGCAGTGGAGGACTCTTTGGAACTACAAATACCACCTCTAATCCTTTT
  GGTAGCACATCTGGCTCCCTTTTTGGGCCAAGTAGTTTTACAGCAGCACCTACAGGAACTACCATCAAAT
  TTAATCCTCCCACTGGTACAGATACTATGGTCAAAGCTGGAGTTAGCACTAACATCAGTACAAAGCATCA
  GTGTATTACTGCTATGAAAGAATATGAAAGCAAGTCATTAGAGGAACTACGTTTGGAGGATTATCAGGCT
  AACCGGAAAGGCCCACAGAACCAAGTGGGAGGAGGCACCACGGCTGGCTTATTTGGGTCTTCTCCAGCAA
  CTTCCAGTGCAACAGGGCTCTTCAGCTCCTCCACCACTAATTCAGCCTTTTCATATGGTCAGAACAAAAC
  TGCCTTTGGAACTAGCACAACTGGATTTGGAACAAATCCAGGTGGTCTCTTTGGCCAACAGAATCAACAG
  ACTACCAGTCTCTTCAGCAAACCATTTGGCCAGGCTACAACCACCCCGAATACTGGCTTTTCCTTTGGTA
  ATACCAGCACCCTTGGACAGCCAAGCACCAATACTATGGGCCTATTTGGAGTAACCCAAGCCTCACAACC
  AGGAGGTCTTTTTGGGACAGCTACAAACACCAGCACTGGGACAGCATTTGGGACAGGAACAGGTCTCTTT
  GGGCAGCCCAATACTGGATTTGGTGCAGTTGGTTCGACCCTGTTTGGCAATAACAAGCTTACAACTTTTG
  GAACCAGCACAACCAGTGCTCCTTCATTTGGTACAACCAGTGGCGGGCTCTTCGGTAACAAACCAACCCT
  GACTTTAGGAACCAATACAAACACTTCCAATTTTGGGTTTGGCACAAATAACAGTGGGAGCAGTATTTTT
  GGAAGTAAGCCAGCAGCTGGAACTTTGGGAACTGGACTTGGTACAGGATTTGGAACAGCTCTTGGTGCTG
  GACAGGCATCTTTGTTTGGAAACAACCAACCTAAGATTGGAGGGCCTCTTGGTACAGGAGCCTTTGGGGC
  CCCTGGATTTAATACTTCGACAGCCATTTTGGGCTTTGGCGCCCCCCAGGCCCCAGTAGCTTTGACAGAT
  CCAAATGCTTCTGCTGCCCAGCAGGCTGTTCTCCAGCAGCACCTCAATAGCCTAACATACTCACCCTTTG
  GAGACTCCCCCCTCTTCCGGAATCCTATGTCAGATCCTAAGAAGAAAGAAGAGAGACTGAAACCAACCAA
  TCCAGCTGCTCAGAAAGCTCTTACAACACCTACTCATTATAAACTTACACCTCGCCCTGCTACCAGAGTC
  AGGCCAAAGGCTTTGCAAACAACAGGCACAGCCAAATCACATCTCTTTGATGGGCTGGATGACGATGAAC
  CATCTCTAGCCAACGGAGCATTCATGCCTAAAAAGAGCATCAAGAAGTTGGTTTTGAAAAATCTCAACAA
  TAGCAATCTCTTTTCTCCTGTTAATCATGATTCAGAAGATCTAGCTTCACCCTCTGAGTATCCAGAAAAT
  GGAGAAAGATTTAGCTTCCTGAGCAAACCTGTTGATGAGAACAATCAGCAGGATGGAGAAGATGACTCTC
  TTGTATCACGATTTTACACTAATCCTATTGCCAAACCCATTCCACAAACTCCAGAGAGTGTTGGAAACAA
  AAATAACAGTAGCAGCAATGTGGAAGATACCATTGTTGCCTTGAACATGCGTGCTGCTTTGCGCAATGGA
  TTGGAAGGAAGCAGTGAAGAGACGTCATTCCATGATGAGTCATTGCAAGATGACCGAGAAGAGATAGAAA
  ATAATGCTTACCACATACACCCAGCAGGCATTGTTCTCACAAAAGTTGGTTATTACACTATCCCATCTAT
  GGATGACCTTGCTAAAATTACCAACGAGAAGGGAGAATGCATTGTTTCTGACTTCACCATTGGTCGTAAA
  GGATATGGCTCAATCTATTTTGAAGGAGATGTGAATTTGACAAATCTAAATTTGGATGATATTGTGCATA
  TCCGAAGGAAAGAAGTTATTGTCTATGTAGATGATAACCAAAAGCCACCTGTGGGTGAAGGGCTAAATAG
  GAAGGCTGAAGTTACTTTGGATGGAGTTTGGCCAACAGATAAAACATCCCGGTGTTTAATAAAGAGTCCA
  GATCGACTTGCTGATATCAACTATGAGGGGAGATTAGAAGCAGTCTCAAGAAAGCAAGGGGCCCAATTCA
  AGGAGTATCGGCCTGAAACTGGTTCTTGGGTATTTAAGGTCTCCCATTTTTCCAAGTATGGCCTTCAGGA
  TTCTGATGAAGAGGAGGAGGAACACCCACCCAAAACGACTTCAAAGAAGCTGAAGACTGCCCCTTTGCCC
  CCTGCAGGCCAGGCAACCACTTTCCAGATGACTCTTAATGGCAAGCCTGCACCCCCACCTCAGGTAGAGA
  AGGGCAGAGAATGTGAATGCAGCTGTGGTAAAGAAGTCAGCTGTGCAACCCTGGCTGCTATTTTTTACTC
  AAGATTTAAAGGGTTTTCTTGCTGTTTGGATCATTCTACAGATTGTAACTTTCAATTTCTCTCATTTGGA
  CTTGTAATGTTTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001287167
• Insert Size: 2955 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001287167.1, NP_001274096.1
• RefSeq Size: 3961 bp
• RefSeq ORF: 2955 bp
• Locus ID: 269966
• Cytogenetics: 7 54.71 cM
• Gene Summary: Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. NUP98 and NUP96 are involved in the bidirectional transport across the NPC. May anchor NUP153 and TPR to the NPC. In cooperation with DHX9, plays a role in transcription and alternative splicing activation of a subset of genes. Involved in the localization of DHX9 in discrete intranuclear foci (GLFG-body).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (5) differs in the 5' UTR, lacks several exons, and its 3' terminal exon extends past a splice site that is used in variant 1. This results in a novel 3' UTR, compared to variant 1. It encodes isoform 5 which is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.