Opa1 (NM_001199177) Mouse Untagged Clone

CAT#: MC229246

Opa1 (untagged) - Mouse optic atrophy 1 (Opa1), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Opa1
• Synonyms: 1200011N24Rik; AI225888; AI847218; lilr3; mKIAA0567
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229246 representing NM_001199177
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCGAGCAGGTCGGGCGGCCGTGGCCTGTGAAGTCTGCCAATCCTTAGTGAAACACAGTTCTGGAA
  TACAAAGAAACGTACCGCTCCAAAAACTCCATCTGGTTTCACGAAGTATTTATCGTTCACATCATCCTGC
  CCTCAAGCTTCAAAGACCCCAACTAAGGACACCATTTCAGCAGTTCTCTTCTCTAACTCACCTTTCATTA
  CATAAATTGAAACTTTCTCCAATTAAATATGGCTACCAGCCCCGCAGGAACTTTTGGCCAGCAAGGTTAG
  CTGCAAGACTCTTAAAACTTCGATATATCATACTGGGATCTGCTGTTGGAGGTGGCTATACAGCCAAAAA
  AACCTTCGATGAATGGAAAGATATGATACCAGACCTTAGTGACTATAAGTGGATTGTGCCTGACTTTATA
  TGGGAAATTGATGAGTATATTGATTTGGAGAAAATTAGAAAAGCCCTGCCCAGCTCAGAAGACCTTGCCA
  GTTTAGCTCCCGACCTGGACAAGATTACTGAGAGCCTCAGCTTGTTGAAGGACTTCTTCACTGCAGGTCC
  CAAATTGGTTAGTGAAGTCCTAGAAGTTTCTGAGGCCCTTCTCTTGTTAGGTTCACCTGGAGAAACAGCA
  TTTCGAGCAACAGATCATGGATCTGAAAGTGACAAGCATTACAGGAAGGTGTCAGACAAAGAAAAGATTG
  ACCAACTTCAAGAAGAACTTCTGCATACTCAGTTAAAGTATCAGAGGATCTTGGAGCGCCTGGAAAAGGA
  GAACAAAGAGCTGCGGAAGCTGGTGCTGCAGAAGGACGACAAAGGCATCCACCACAGGAAGCTCAAGAAA
  TCTTTGATTGATATGTATTCTGAAGTTCTTGATGTTCTTTCTGATTATGATGCCAGTTACAATACACAAG
  ATCACCTACCACGGGTTGTTGTGGTTGGAGATCAGAGTGCTGGGAAAACCAGTGTGCTGGAAATGATTGC
  TCAGGCCCGGATCTTCCCGAGAGGGTCCGGCGAGATGATGACACGCTCTCCAGTGAAGGTGACTCTCAGT
  GAAGGCCCTCACCATGTGGCCTTGTTTAAAGATAGCTCTCGGGAATTTGATCTCACCAAGGAGGAAGATC
  TTGCAGCATTAAGACATGAAATCGAACTCCGAATGAGGAAAAATGTGAAAGAAGGTTGTACTGTTAGCCC
  CGAGACCATATCTCTAAATGTCAAAGGCCCTGGGCTGCAGAGGATGGTGCTCGTGGACTTGCCTGGTGTC
  ATCAACACCGTGACATCAGGCATGGCTCCCGACACAAAGGAAACTATTTTCAGTATCAGCAAAGCTTACA
  TGCAGAATCCTAACGCCATCATCCTGTGCATCCAAGACGGATCCGTAGATGCTGAGCGCAGTATTGTTAC
  AGACTTGGTCAGTCAAATGGATCCTCATGGAAGAAGAACCATATTTGTTTTGACCAAAGTAGACCTGGCA
  GAAAAAAATGTAGCCAGTCCAAGCAGGATACAACAGATAATTGAAGGCAAGCTCTTCCCAATGAAAGCTC
  TGGGTTATTTTGCTGTCGTAACAGGAAAAGGAAACAGCTCTGAAAGTATTGAAGCTATAAGAGAGTATGA
  AGAGGAATTTTTTCAGAATTCAAAACTGCTAAAGACAAGCATGCTAAAGGCACACCAGGTCACCACGAGA
  AATCTCAGCCTTGCTGTGTCAGACTGCTTTTGGAAAATGGTTCGAGAGTCAGTTGAACAACAGGCTGATA
  GTTTTAAAGCCACGCGCTTTAACCTAGAGACGGAATGGAAGAATAACTACCCGCGCCTGCGAGAGCTCGA
  CAGGAATGAACTCTTTGAAAAAGCTAAAAATGAGATCCTCGATGAGGTCATCAGTCTGAGCCAGGTCACG
  CCAAAGCACTGGGAGGAAATCCTGCAGCAATCCCTGTGGGAACGAGTGTCAACACATGTGATTGAGAACA
  TCTACCTTCCAGCTGCCCAGACCATGAATTCGGGAACATTTAACACCACAGTAGACATCAAGCTTAAACA
  GTGGACTGACAAGCAGCTTCCTAATAAAGCAGTCGAGGTTGCCTGGGAGACTCTACAAGAGGAATTTTCC
  CGCTTCATGACAGAACCCAAAGGAAAGGAACACGACGACATATTTGACAAACTTAAGGAGGCTGTGAAGG
  AGGAGAGTATCAAGCGGCACAAGTGGAACGACTTTGCCGAGGATAGCTTGAGGGTTATTCAGCACAATGC
  TTTGGAAGACCGGTCCATATCAGATAAGCAACAGTGGGATGCAGCCATTTACTTCATGGAAGAGGCGCTT
  CAAGGTCGTCTCAAGGATACTGAAAATGCTATTGAAAACATGATTGGGCCAGACTGGAAAAAGAGGTGGA
  TGTACTGGAAGAATCGGACCCAAGAGCAGTGTGTTCACAACGAAACCAAGAACGAGTTGGAGAAGATGCT
  GAAGGTTAATGATGAGCACCCAGCTTACCTGGCAAGTGATGAGATTACCACAGTCCGGAAGAACCTGGAG
  TCTCGAGGAGTGGAAGTCGATCCAAGCTTGATTAAGGATACTTGGCATCAAGTTTATAGAAGACATTTCT
  TAAAAACAGCTCTAAATCATTGTAACCTTTGTCGCAGAGGTTTTTATTACTACCAGAGGCATTTTATAGA
  TTCTGAGCTGGAATGCAATGACGTGGTCCTGTTTTGGCGAATACAGCGCATGCTCGCTATCACTGCCAAT
  ACATTAAGGCAGCAGCTTACAAACACTGAAGTTAGGCGACTAGAGAAAAACGTTAAAGAGGTATTAGAAG
  ATTTTGCAGAAGACGGTGAGAAGAAGGTTAAATTGCTCACTGGCAAACGAGTTCAGCTGGCAGAAGATCT
  CAAGAAAGTTAGAGAAATTCAAGAAAAGCTTGATGCTTTCATTGAAGCTCTTCACCAGGAGAAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001199177
• Insert Size: 2937 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001199177.1, NP_001186106.1
• RefSeq Size: 6002 bp
• RefSeq ORF: 2937 bp
• Locus ID: 74143
• Cytogenetics: 16 20.65 cM
• Gene Summary: Dynamin-related GTPase that is essential for normal mitochondrial morphology by regulating the equilibrium between mitochondrial fusion and mitochondrial fission (PubMed:11847212, PubMed:24616225, PubMed:26785494, PubMed:28746876). Coexpression of isoform 1 with shorter alternative products is required for optimal activity in promoting mitochondrial fusion (By similarity). Binds lipid membranes enriched in negatively charged phospholipids, such as cardiolipin, and promotes membrane tubulation. The intrinsic GTPase activity is low, and is strongly increased by interaction with lipid membranes (By similarity). Plays a role in remodeling cristae and the release of cytochrome c during apoptosis (PubMed:16839884, PubMed:16839885). Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space (PubMed:16839884, PubMed:16839885). Plays a role in mitochondrial genome maintenance (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.