Gtf2ird1 (NM_001244936) Mouse Untagged Clone

CAT#: MC229238

Gtf2ird1 (untagged) - Mouse general transcription factor II I repeat domain-containing 1 (Gtf2ird1), transcript variant 11

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gtf2ird1
• Synonyms: 1700012P16Rik; BEN; Cream1; ESTM9; Gtf2il; GTF3; MusTRD1; Tg(Alb1-Myc)166.8Sst; WBSCR11; X83320
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229238 representing NM_001244936
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTTGCTGGGGAAGCACTGTGACATCCCCACCAACGGCTGTGGGTCTGAGCGCTGGAACTCCACCT
  TCGCCCGCAAGGACGAACTCATCAACAGTCTGGTGTCCGCCTTAGACTCCATGTGCTCGGCGCTCTCCAA
  GCTGAACACGGAGGTGGCCTGCGTGGCGGTACACAATGAGAGCGTCTTCGTGATGGGCACCGAGAAGGGA
  AGGGTGTTTCTGAACACTCGGAAGGAGCTACAGTCAGACTTCCTCAGGTTCTGCCGGGGACCCCTGTGGA
  ACGATCCAGAAGCAGGACACCCTAAAAAGGTGCAGCGCTGTGAAGGCGGTGGCCGGAGCCTCCCGCGGTC
  CTCTCTGGAGCAGTGCTCGGATGTGTACCTGCTGCAGAAGATGGTAGAGGAAGTGTTTGATGTTCTTTAT
  AGTGAGGCTATGGGCAGGGCCACCGTGGTACCTTTGCCCTATGAGAGGCTGCTCAGGGAGCCGGGGCTAC
  TGGCGGTGCAGGGGCTGCCCGAGGGCCTGGCCTTCCGGAGGCCAGCAGAGTATGACCCCAAGGCACTCAT
  GGCCATATTGGAGCACAGTCACCGAATTCGGTTTAAGCTCAGGAGGCCTCCTGATGACGGTGGGCAGGAC
  ACGAAGGCGCTGGTGGAGATGAACGGTATCTCTCTGCTACCCAAGGGGTCCCGAGACTGTGGTCTGCATG
  GCCAGGCCTCCAAGGTCGCTCCCCAAGACCTGACCCCCACCGCCACCCCATCCTCTATGGCCAACTTCCT
  GTACAGCACTTCGATGCCCAACCACACGATCCGGGAACTCAAGCAGGAGGTGCCAACCTGCCCGTTGACC
  CCCAGCGACCTGGGCATGGGCTGGCCCGTGCCTGAGCCCCATGTCCCCAGCACCCAAGATTTCTCTGATT
  GCTGTGGACAGACGCCTGCAGGGCCTGCTGGGCCTCTCATCCAGAATGTCCATGCTTCCAAGCGCATCCT
  CTTCTCCATCGTCCATGACAAGTCAGAGAAGTGGGATCCCTTCATCAAGGAAATGGAGGACATCAATACC
  CTGCGGGAGTGCGTGCAGATTCTGTTTAACAGCAGATACGCGGAAGCCCTGGGCCTGGACCACATGGTCC
  CTGTGCCCTATAGGAAGATTGCCTGTGACCCCGAGGCTGTGGAAATTGTGGGTATTCCAGACAAGATCCC
  CTTCAAGCGACCCTGTACTTACGGAGTGCCGAAGCTGAAGAGGATTCTGGAGGAGCGACACAGCATTCAC
  TTCATTATCAAGAGAATGTTCGATGAGCGCATTTTCACAGGGAACAAGTTTACCAAAGACCCCATGAAGC
  TGGAGCCAGCTAGCCCACCAGAAGACACTTCCACAGAAGTCTGTAGGGACAGCATGCTGGACCTGGCTGG
  GACTGCTTGGTCAGACATGAGCAGCGTCTCTGAAGACTGTGGGCCAGGAACCTCAGGAGAGATAGCAATG
  TTGAGGCCTATCAAAATCGAGCCAGAGGAGCTGGACATTATTCAGGTTACGGTCTCAGATCCTTCACCTA
  CCTCTGAGGAGATGACTGACTCGTTACCTGGGCATCTGCCCTCAGAGGATTCCGGTTATGGGATGGAAAT
  GCCGGCTGACAAAGGCCCCAGTGAAGAACCGTGGTCAGAAGAGAGGCCGGCCGAAGAGAGCCCTGGTGAC
  GTGATCCGGCCCCTACGGAAGCAGGTGGAGATGCTGTTCAACACGAAATATGCCAAAGCTATTGGTACCT
  CAGAGCCGGTCAAGGTGCCCTACTCCAAGTTCCTGATGCACCCGGAGGAGCTGTTCGTACTGGGACTGCC
  TGAAGGCATCTCTCTTCGGAGACCCAACTGCTTTGGGATTGCAAAGCTGCGGAAGATTCTGGAAGCGAGC
  AACAGCATCCAGTTTGTCATCAAGAGACCCGAACTGCTCACTGACGGTGTCAAAGAACCTGTTCTGGACA
  CTCAAGAGAGGGACTCCTGGGACCGTCTTGTGGACGAGACCCCGAAGAGACAGGGCCTTCAAGAAAATTA
  CAACACCAGACTCTCGCGGATCGACATCGCCAACACGCTTAGGGAACAAGTCCAAGACCTGTTTAACAAG
  AAATACGGTGAAGCTCTGGGCATCAAATACCCAGTGCAGGTGCCCTACAAGAGAATCAAAAGCAACCCAG
  GCTCGGTAATCATTGAAGGCCTACCCCCCGGGATCCCATTCCGCAAACCCTGCACCTTTGGCTCCCAGAA
  CCTGGAAAGGATTCTCTCTGTGGCTGACAAGATCAAGTTCACGGTCACCAGGCCATTCCAAGGACTTATC
  CCAAAGCCTGATGAGGATGATGCCAACAGACTGGGGGAGAAGGTGATCCTCCGAGAGCAGGTGAAGGAGC
  TCTTCAATGAGAAATACGGTGAGGCCCTGGGACTGAATCGGCCTGTGCTGGTCCCTTACAAACTGATCCG
  GGACAGCCCAGATGCCGTGGAGGTGAAGGGCCTCCCAGATGACATCCCCTTCCGGAACCCCAACACCTAT
  GACATCCATCGGCTGGAGAAGATCCTGAAGGCCAGGGAGCATGTGCGGATGGTCATCATCAACCAGCTCC
  AACCTTTTGGGGACGTCTGCAACAATGCCAAGGTGCCAGCCAAAGACAACATTCCCAAGCGCAAGAGAAA
  GAGGGTCTCTGAAGGCAACTCAGTCTCCTCTTCTTCCTCCTCTTCATCTTCGTCCTCTAACCCAGAGTCT
  GTGGCATCCACCAACCAGATCTCCCTCGTGGTAAAGTCACGCGGATCCGAACTTCACCCTAATTCTGTGT
  GGCCTTTGCCCCTTCCTCGGGCAGGGCCATCCACAGCTCCAGGAACTGGGAGGCACTGGGCTCTGAGAGG
  GACTCAGCCAACCACAGAGGGGCAGGCACATCCACTCGTTTTGCCCACAAGATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001244936
• Insert Size: 2925 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001244936.1, NP_001231865.1
• RefSeq Size: 4321 bp
• RefSeq ORF: 2925 bp
• Locus ID: 57080
• UniProt ID: Q9JI57
• Cytogenetics: 5 74.55 cM
• Gene Summary: May be a transcription regulator involved in cell-cycle progression and skeletal muscle differentiation. May repress GTF2I transcriptional functions, by preventing its nuclear residency, or by inhibiting its transcriptional activation. May contribute to slow-twitch fiber type specificity during myogenesis and in regenerating muscles. Binds troponin I slow-muscle fiber enhancer (USE B1). Binds specifically and with high affinity to the EFG sequences derived from the early enhancer of HOXC8.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (11) differs in the 3' coding region and the 3' UTR, as compared to variant 1. The encoded isoform (j) lacks two internal segments and has a longer and distinct C-terminus, as compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.