Chrd (NM_001278041) Mouse Untagged Clone

CAT#: MC229235

Chrd (untagged) - Mouse chordin (Chrd), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Chrd
• Synonyms: Chd
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229235 representing NM_001278041
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGAGCCTCCCGGCCCCGCCGGCCCCGCGGCTGCTCCTCGGGCTGCTGCTGCTCGGCTCGCGGCCGG
  CCAGTGGCACTGGCCCTGAGCCCCCAGCACTGCCCATCCGTTCCGAGAAGGAGCCGCTGCCTGTCCGGGG
  AGCGGCAGAGCCCGGAGCAGGCATTACGGCACGAGCAACCGGACAGCGGCCGCCAGCCAAGCCCGTCCCC
  GCAGGCTGCTCCTTCGGCGGGAAGGTCTATGCCTTGGACGAGACGTGGCACCCGGACCTGGGGGAGCCTT
  TTGGGGTGATGCGCTGCGTGCTGTGCGCCTGTGAAGCGCCTCAGTGGGCTCGCCGTGGGAGGGGTCCTGG
  CAGGGTCAGCTGCAAGAACATCAAACCTCAGTGCCCCACCCTGGCCTGCAGGCAGCCGCGCCAGCTGCCA
  GGACACTGCTGCCAGACCTGCCCGCAGGAGCGCAGCAATCTAGATCCACAGCCCGCTGGCCTGGTCTTCG
  AGTATCCAAGGGATCCAGAGCATCGCAGTTACAGTGATCGAGGGGAACCCGGCGTTGGGGAGCGGACCCG
  TGCTGATGGCCACACAGACTTTGTGGCGCTGCTGACAGGACCGAGGTCGCAGGCGGTAGCTCGTGCTCGA
  GTCTCTTTGCTGCGCTCAAGTTTACGCTTCTCTGTCTCCTACCAGCGGCTGGACCGTCCCAGCAGGGTTC
  GTTTCACAGATCCCACAGGCAACATCCTGTTTGAACACCCTGCAACCCCCACCCAGGATGGCCTGGTCTG
  TGGGGTGTGGCGGGCAGTGCCTCGGCTGTCTGTGAGGCTCCTGAGGGCAGAGCAGCTTCGGGTAGCCCTT
  GTGACATCCACTCACCCTTCAGGAGAAGTCTGGGGGCCTCTCATTTGGCAGGGTGCTCTCGCTGCAGAGA
  CCTTCAGTGCCATCCTGACCCTGGAAGACCCACTGCAGCGGGGTGTGGGGGGCATAGCCCTGCTCACCCT
  CAGTGACACAGAAGATTCCTTGCATTTTTTGCTGCTCTTCCGGGGTCTGCTGGGAGGACTAGCTCAGGCT
  CCCTTGAAGCTTCAGATTCTCCACCAGGGACAGCTACTTCGGGAGCTCCAGGCCAACACCTCCGCTCAGG
  AGCCAGGTTTTGCTGAGGTGCTGCCCAGCCTTACAGACCAAGAGATGGACTGGTTGGAGCTGGGGGAGCT
  GCAGATGGTCCTAGAGAAGGCGGGTGGGCCAGAGCTACGCATCAGTGGATACATCACCACCAGGCAGAGC
  TGTGATGTCCTTCAAAGTGTCCTTTGTGGGGCTGATGCCCTGATCCCAGTCCAGACGGGTGCTGCTGGGT
  CAGCCAGCTTCATATTGCTAGGAAATGGCTCCCTTATCTATCAGGTGCAAGTGGTAGGTACAGGTAGCGA
  GGTGGTGGCCATGACACTGGAGACCAAGCCTCAGCGGAAGAACCAGCGCACTGTCCTGTGCCACATGGCT
  GGACTCCAGCCGGGAGGACACATGGCTGTGGGTATGTGCTCTGGGCTGGGTGCCCGAGGGGCTCATATGC
  TGCTACAGAACGAGCTGTTCCTGAATGTTGGTACCAAGGACTTCCCAGATGGAGAGCTTCGGGGGCATGT
  GACTGCCCTATGCTACAGTGGGCACAGTGCCCGCTATGACAGATTGCCTGTGCCTCTGGCAGGGGCACTA
  GTGCTGCCCCCTGTGCGGAGTCAGGCAGCAGGGCACGCCTGGCTCTCCTTGGATACACATTGTCACTTAC
  ACTATGAAGTTCTATTGGCTGGGCTTGGTGGCTCAGAGCAAGGAACTGTCACTGCCCACCTCCTTGGGCC
  TCCTGGGATGCCAGGGCCCCAGCGGCTGCTGAAGGGATTCTATGGCTCAGAGGCTCAGGGCGTGGTAAAA
  GATCTGGAGCCTGTGCTGCTGCGGCACCTGGCACAGGGCACTGCCTCCCTGCTCATCACCACCAAGAGTA
  GCCCCAGAGGAGAACTACGTGGGCAGGTGCACATTGCCAGTCAGTGTGAGGCGGGAGGCCTGCGTCTGGC
  CTCAGAAGGAGTGCAGATGCCCTTGGCTCCAAATGGAGAGGCGGCTACGTCACCCATGCTGCCTGCTGGC
  CCTGGCCCTGAAGCCCCAGTACCAGCCAAACATGGCAGCCCTGGGAGACCCCGAGATCCTAACACATGTT
  TCTTCGAGGGGCAGCAGCGGCCCCACGGGGCTCGCTGGGCACCCAACTATGACCCACTCTGCTCCCTCTG
  CATCTGCCAGAGACGAACAGTGATCTGTGACCCTGTAGTATGCCCACCACCAAGCTGTCCCCACCCGGTG
  CAGGCACTGGACCAGTGCTGTCCCGTGTGTCCAGAGAAACAACGCAGTAGAGACCTCCCCAGCCTACCAA
  ATCTGGAGCCAGGAGAGGGCTGCTATTTTGATGGTGACCGGAGCTGGAGGGCAGCGGGTACCCGATGGCA
  CCCTGTTGTGCCCCCCTTTGGCCTAATTAAGTGTGCTGTCTGCACCTGCAAGGGGGCCACGGGAGAGGTG
  CACTGTGAGAAGGTGCAGTGTCCTCGCCTGGCCTGTGCTCAGCCTGTCCGTGCCAACCCCACCGACTGCT
  GCAAACAGTGTCCAGTAGGGTCAGGGACTAATGCCAAGCTGGGAGACCCCATGCAGGCTGATGGGCCTCG
  GGGGTGTCGCTTTGCTGGGCAGTGGTTCCCAGAGAATCAGAGCTGGCACCCATCAGTGCCCCCCTTTGGG
  GAGATGAGCTGTATTACCTGCAGATGTGGGGCAGGGGTACCCCACTGTGAGCGGGACGATTGTTCACCGC
  CGCTATCCTGTGGCTCAGGGAAGGAGAGTCGATGCTGTTCCCACTGCACAGCCCAAAGGTCCTCTGAGAC
  TAGAACCCTTCCAGAGCTGGAGAAAGAAGCGGAGCACTCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001278041
• Insert Size: 2913 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001278041.1, NP_001264970.1
• RefSeq Size: 3358 bp
• RefSeq ORF: 2913 bp
• Locus ID: 12667
• UniProt ID: Q9Z0E2
• Cytogenetics: 16 12.51 cM
• Gene Summary: Dorsalizing factor. Key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta family bone morphogenetic proteins (BMPs) and sequestering them in latent complexes.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame acceptor splice site in the 5' coding region compared to variant 1, which results in a longer isoform (2) containing an internal protein segment not found in isoform 1.