Arhgef2 (NM_001198911) Mouse Untagged Clone

CAT#: MC229233

Arhgef2 (untagged) - Mouse rho/rac guanine nucleotide exchange factor (GEF) 2 (Arhgef2), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arhgef2
• Synonyms: AA408978; GEF; GEF-H1; GEFH1; Lbcl1; Lfc; LFP40; mKIAA0651; P40
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229233 representing NM_001198911
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCGGCAGGGACAAGACCCGGGAAAAGGAGAAGATGAAGGAAGCTAAAGATGCCCGCTATACCAACG
  GCCACCTCTTCACCACCATCTCCGTCTCCGGCATGACCATGTGCTATGCCTGTAACAAGAGCATCACAGC
  CAAGGAAGCCCTCATTTGTCCTACATGTAACGTGACCATCCACAACCGCTGTAAAGACACCCTGGCCAAC
  TGTACCAAGGTCAAGCAGAAGCAACAGAAAGCTGCACTGCTGAGGAACAACACTGCTTTGCAGTCTGTCT
  CCCTTCGAACGACCACCAGAGAGCGGCCAACGTCTGCCATTTACCCTTCCGACAGCTTCCGGCAGTCCCT
  CCTGGGTTCTCGGCGGGGCCTCTCCTCCTTATCTTTGGCCAAAAGTGTTTCCACTACCAACATTGCTGGA
  CATTTCAATGATGAGTCTCCTCTGGGGCTGCGTCAGATCCTCTCCCAGTCCACAGACTCCCTCAACATGC
  GGAACCGAACCCTGTCCGTGGAATCCCTTATTGATGAAGGTGTAGAAGTGTTCTACAATGAGCTGATGAG
  CGACTTTGAGATGGATGAGAAGGACTTTGAGGCGGATTCTTGGAGCCTTGCCGTGGACAGCAGCTTCCTG
  CAGCAGCACAAAAAGGAAGTGATGAAGAAGCAAGATGTCATCTATGAGCTGATCCAGACAGAGCTGCACC
  ACGTGAGAACCTTGAAGATTATGACCCGCCTCTTTCGCACTGGGATGCTGGAAGAGTTGCAGATGGAGCC
  AGAAGTGGTCCAGGGCCTGTTCCCCTGCGTGGATGAACTCAGTGACATTCACACACGTTTCCTTAATCAG
  CTTCTGGAACGGCGGCGCCAGGCTCTATGTCCAGGCAGCACCCGGAACTTTGTCATCCATCGTTTGGGTG
  ACTTGCTCATCAGTCAGTTCTCAGGTTCCAATGCTGAGCAGATGCGCAAGACCTACTCAGAGTTCTGCAG
  CCGCCACACCAAGGCCTTAAAGCTCTATAAGGAGCTGTATGCTCGAGACAAACGCTTCCAACAGTTCATC
  CGGAAAATGACCCGCTCAGCTGTGTTGAAGCGGCATGGAGTTCAGGAATGCATTCTCCTGGTGACTCAGC
  GGATCACCAAATACCCTGTGCTCATCAACAGAATCCTGCAGAATTCCCACGGGGTTGAAGAAGAGTACCA
  AGACTTGGCGTCAGCCCTAGGACTAGTGAAGGAGTTGTTGTCCAATGTGGACCAGGATGTGCACGAGCTG
  GAGAAAGAGGCCCGCCTGCAGGAGATTTACAACCGAATGGATCCCCGGGCTCAGACCCCCGTACCTGGCA
  AGGGCCCCTTCGGCCGAGATGAACTTTTACGGAGAAAACTTATCCATGAAGGCTGCCTGCTCTGGAAGAC
  AGCCACAGGCCGCTTCAAAGATGTCCTGTTGCTGCTGATGACAGATGTGCTCGTGTTTCTCCAGGAAAAG
  GACCAGAAATACATTTTCACGTCTCTGGACAAGCCCTCAGTGGTGTCCTTGCAGAACCTCATCGTAAGAG
  ACATAGCCAACCAGGCGAAAGGGATGTTTCTGATTAGTTCTGGACCGCCTGAGATGTATGAGGTGCATGC
  AGCGTCCCGAGACGACCGGACTACCTGGATCCGTGTCATCCAGCAGAGTGTGCGCCTGTGCCCGTCCAGG
  GAGGACTTTCCTCTGATCGAGACAGAGGATAAGGCGTATCTCCGGAGGATCAAGACGAAACTGCAGCAGA
  AAAACCAGGCGCTAGTGGAGCTGCTACAGAAGAATGTTGAGCTGTTTGCCGAGATGGTCCACTTCCAGGC
  CTTAAAGGCTGGCTTCGTTGGAATGCCCCCACCCGCCCTGCCCAGGGGCCTTTTCCGTCTTGAGTCCTTT
  GAGTCCCTCCGAGGCGAGCGCCTGCTAAAGGATGCCCTCCGTGAAGTGGAAGGCCTGAAAGACCTGCTGT
  TGGGCCCATGTGTGGACCTGCCTATGACATCCCGAGAACCAGCCTTACCCTTGGACTCTGACAGCGGTAG
  CTGTCCTGGGGTCACTGCCAATGGAGAGGCCAGAACCTTCAATGGCTCCATTGAACTCTGTAGAGCAGAC
  TCGGATTCCAGCCAGAAGGATCGGAATGGAAATCAGTTGAGATCACCACAGGAGGAGGTGTTACAGCCAT
  TGATCAATCTTTATGGACTTCTACATGGCCTGCAGGCTGTTGTGGTCCAGCAGGAAAGACTGATGGAAGC
  CCTGTTCCCTGAGGGCCCTGAACGGTGGGAAAAGCTATCCCGAGCCAACTCTCGGGATGGTGAAGCTGGC
  CGGGCTGCGGTTGCTTCTGTAACTCCTGAGAAGCAGGCCACGGAGCTGGCACTACTGCAGAGGCAACACA
  CCCTGTTGCAGGAAGAGCTGCGGCGCTGCCAGCGGCTCGGGGAAGAGCGGGCAACTGAAGCTGGCAGCCT
  GGAGGCCAGGCTCCGAGAGAGCGAGCAAGCCCGGGCCCTGCTGGAGCGGGAGGCTGAAGAGATCCGCCGG
  CAGCTTGCAGCCTTGGGCCAAAACGAGCCACTCCCGGCAGAAGCGCCCTGGGCTCGCAGGCCTCTGGACC
  CACGGCGCCGCAGCCTTCCAGCGGGCGACGCTTTATACTTGAGCTTCAATCCCCCCCAGCCCAGTCGAGG
  CCATGACCGCCTGGATTTGCCTGTGACTGTTCGTTCCCTCCACCGACCCTTTGATGACCGAGAGGCGCAA
  GAACTTGGTAGCCCCGAGGATCGACTACAGGACAGCAGTGACCCTGATACTGGTAGTGAGGAGGAAGTCA
  GTAGCCGCCTGTCTCCACCTCACAGTCCTCGAGACTTCACCCGAATGCAGGACATTCCTGAAGAGACAGA
  AAGCCGAGATGGGGAGCCCACAGCTTCAGAGAGCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001198911
• Insert Size: 2907 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001198911.1, NP_001185840.1
• RefSeq Size: 4422 bp
• RefSeq ORF: 2907 bp
• Locus ID: 16800
• UniProt ID: Q60875
• Cytogenetics: 3 F1
• Gene Summary: Activates Rho-GTPases by promoting the exchange of GDP for GTP. May be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, innate immune response, and cancer. Binds Rac-GTPases, but does not seem to promote nucleotide exchange activity toward Rac-GTPases. May stimulate instead the cortical activity of Rac. Inactive toward CDC42, TC10, or Ras-GTPases. Forms an intracellular sensing system along with NOD1 for the detection of microbial effectors during cell invasion by pathogens. Involved in innate immune signaling transduction pathway promoting cytokine IL6/interleukin-6 and TNF-alpha secretion in macrophage upon stimulation by bacterial peptidoglycans; acts as a signaling intermediate between NOD2 receptor and RIPK2 kinase. Contributes to the tyrosine phosphorylation of RIPK2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Overexpression activates Rho-, but not Rac-GTPases, and increases paracellular permeability (By similarity). Involved in neuronal progenitor cell division and differentiation (PubMed:28453519). Involved in the migration of precerebellar neurons (PubMed:28453519).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) has multiple differences at its 5' end, including a different translation initiation site, compared to variant 1. Variant 2 encodes a shorter protein (isoform 2) with a different N-terminus, compared to isoform 1.