Mib2 (NM_001256107) Mouse Untagged Clone

CAT#: MC229214

Mib2 (untagged) - Mouse mindbomb homolog 2 (Drosophila) (Mib2), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mib2
• Synonyms: 2210008I11Rik; skd; Zzank1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229214 representing NM_001256107
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCTGGACCCCCATGCAGGTGTGCAGGTGGGCATGCGTGTGGTACGCGGAATGGACTGGAAATGGG
  GCCAGCAGGATGGAGGTGAGGGCGGTGTGGGCACCGTGGTGGAGCTTGGCCGCCATGGCAGCCCCTCGAC
  CCCCGACCGTACAGTTGTCGTTCAGTGGGACCAGGGCACACGTACCAACTATCGAGCTGGCTACCAAGGT
  GCCCATGACCTGTTGCTCTATGACAACGCCCAAATCGGTATCCGCCACCCCAATATCATCTGTGACTGCT
  GCAAGAAACATGGGCTTCGGGGCATGCGTTGGAAGTGCCGCGTCTGCTTTGACTATGACCTCTGCACACA
  GTGCTACATGCACAACAAGCATGACCTGACCCATGCCTTCGAGCGCTATGAGACATCTCACTCACGCCCG
  GTTACGCTGAGCCCCCGACAGGGCCTCCCTCGGATCCCACTGAGGGGCATCTTTCAAGGAGCTAAGGTGG
  TACGAGGCCCTGACTGGGAATGGGGCTCACAAGATGGAGGGGAAGGCAAGACAGGTCGTGTGGTGGACAT
  CCGTGGCTGGGATGTGGAGACAGGCCGAAGTGTGGCCAGTGTGACGTGGGCAGATGGAACCACGAACGTG
  TACCGAGTGGGCCACAAGGGCAAGGTGGATCTCAGATGTGTTGGTGAGGCAGCTGGCGGCTTTTACTACA
  AGGAGCACCTCCCAAAGCTTGGCAAGCCAGCAGAGCTGCAGCGCAGGGTGAGTGCTGATGGCCAGCCCTT
  CCAGCGTGGGGACAAGGTCAAGTGTCTGCTGGACACAGATGTCCTAAGGGATATGCAAGAAGGCCATGGT
  GGCTGGAACCCTAGGATGGCAGAGATGGGCACCGTGCACCGCATCACAGACCGTGGGGACGTGCGTGTGC
  AGTTCAACCACGAGACCCGCTGGACCTTCCACCCTGGGGCTCTCACCAAGCACAACAGCTTCTGGGTGGG
  TGATGTGGTCCGGGTCATCGGTGACCTTGACACTGTGAAGCGACTTCAGGCCGGACATGGTGAATGGACC
  GACGACATGGCTCCCGCCCTGGGCCGAGTGGGGAAAGTGGTGAAGGTGTTTGGAGATGGAAACTTGCGTG
  TGGCGGTTGGCGGTCAGCGGTGGACCTTCAGCCCCTCTTGCCTAGTGGCCTACCGGCCTGAGGAAGACGC
  CAACCTGGATGTGGCTGAGCGTGCCAGAGAGAACAAAAGCTCACTGAGTGTGGCCCTGGACAAGCTTCGG
  ACCCAGAAGAGTGACCCAGAGCACCCGGGGAGGCTGGTAGTAGAGGCCGCACTAGGAAATGTAGCCCGGG
  CTCTAGATCTTCTGCGGAGGCATCCAGAGCAGGTGGACACCAAGAACCAGGGTAGAACTGCCCTGCAGGT
  GGCTGCTTACCTGGGCCAGGTAGAGCTGGTGCGGCTGCTGCTGCAGGCAAGAGCAAGTATGGACCTGCCG
  GATGATGAGGGCAACACTGTGCTGCACTACACAGCCATGGGGAACCAGCCTGAGGCCACAAGGGTGCTCC
  TGAGTGCAGGGTGTGCAGTGGATGCTCGGAATGGCACACGCAGCACAGCCCTACATGTGGCTGTACAGAG
  GGGCTTCCTAGAGGTGGTAAAGATCCTGTGTGAACGTGGTTGTGATGTCAACTTGCCGGATGCGCATGCA
  GACACACCCCTGCATTCTGCCATCTCTGCTGGTGCTGGTGCCAGCAGCATTGTTGAAGTCCTCACCGAGG
  TCCCTGGCATCGATGTCACTGCTACCAATAGCCAGGGCTTCACACTGCTACACCATGCATCCCTCAAGGG
  CCATGTGCTAGCAGTCAGGAAGATTCTGGCAAGGGCCCGGCAGCTGGTGGATGCCAAGAAGGAGGATGGC
  TTTACCGCACTGCATCTGGCAGCCCTCAACAACCACCGGGAGGTGGCCCAGGTCCTAATCCGAGAGGGTC
  GCTGTGATGTGAATGTGCGGAACCGGAAGCTTCAGTCTCCACTTCATCTGGCTGTACAGCAGGCCCACCT
  GGGGCTGGTACCGCTGCTAGTGGACGCAGGCTGCAGTGTCAACACGGAGGACGAGGAGGGTGACACGGCC
  CTGCATGTGGCACTACAGCGTCACCAGCTGTTGCCCCTTGTGGCTGACAGGGCTGGGGGAGACCCAGGGC
  CCTTGCAGCTGCTGTCAAGGCTACAGGCCTCAGGCCTCCCAGGCTGCACAGAGTTGACTGTAGGCGCTGC
  AGTGGCCTGCTTCCTGGCGTTGGAGGGTGCTGATGTGAGCTACGCAAACCACCGCGGCCGGAGCCCACTG
  GACCTGGCCACAGAGGGCCGAGTGCTCAAGGCCTTGCAGGGCTGTGCCCAGCGCTTCAGGGAGCGACAGG
  CAGGTGGCGGTGGGGGTGTGCCCCCGGGCCCCCGGCATGTGCTGAGTACTCCTAACACCGTGACGAACCT
  GCACGTGTCTGGCACGGCAGGGCCAGAAGCTGCCGAGTGCCTGGTGTGCTCGGAGCTGGCATTGCTAATT
  CTGTTCTCACCGTGTCAGCACCGCACAGTGTGTGAGGAGTGCGCTCGAAGGATGAAGAAGTGTATCAGGT
  GCCAGGTGGTCATCAGCAAGAAGCTCCGCCCAGACGGCTCAGAGGTGGTAAATGCCATCCAGGTGCCCGG
  CCCGCCTCGGCAACTGGTGGAGGAGCTACAGAGCCGCTACAGGCAGATGGAGGAGCGCATCACCTGCCCC
  ATCTGCATCGACAGCCACATCCGCCTGGTGTTCCAGTGCGGCCATGGAGCGTGCGCGCCCTGTGGCGCCG
  CGCTTAACGCCTGCCCCATCTGCCGCCAGCCCATCCGCGACCGCATTCAGATCTTCGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001256107
• Insert Size: 2862 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001256107.1, NP_001243036.1
• RefSeq Size: 4019 bp
• RefSeq ORF: 2862 bp
• Locus ID: 76580
• Cytogenetics: 4 E2
• Gene Summary: E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1), as well as variant 4, encodes isoform 1.