Agtpbp1 (NM_001284219) Mouse Untagged Clone

CAT#: MC229211

Agtpbp1 (untagged) - Mouse ATP/GTP binding protein 1 (Agtpbp1), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Agtpbp1
• Synonyms: 1700020N17Rik; 2310001G17Rik; 2900054O13Rik; 4930445M19Rik; 5730402G09Rik; CCP1; nmf243
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229211 representing NM_001284219
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCATTCGGAAGGGGATTCTACAGAGCTTAAAAAGTGTCACGAACATCAAGTTGGGCAGAAAAGCAT
  TTATTGATGCGAATGGGATGAAAATTCTGTATAACACTTCTCAAGAGTGCTTGGCGGTCAGGACTCTTGA
  TCCTCTTGTCAACACATCCAGTCTGATAATGAGAAAATGCTTCCCCAAAAACCGCCTTCCGCTCCCCACC
  ATTAAAAGTTCTTTCCACTTCCAATTGCCAATTATCCCTGTGACTGGACCTGTGGCCCAGCTCTACAGCT
  TGCCGCCTGAAGTGGACGATGTGGTGGACGAGAGTGACGACAACGACGACATTGATTTAGAAGTGGAAAA
  TGAACTCGAGAATGAAGATGACCTAGATCAGAGTTTTAAGAATGATGATATTGAAACAGATATTAATAAA
  TTAAGACCTCAGCAAGTACCAGGACGAACAATAGAAGAACTAAAAATGTACGAGCACCTTTTCCCCGAGC
  TTGTTGATGATTTTCAGGACTATGAACTGATCTCTAAGGAACCCAAACCTTTTGTGTTTGAGGGGAAGGC
  TCGGGGCCCCATTGTAGTTCCCACAGCTGGAGAGGAGGTACCTGGGAATTCAGGGAGCGTAAAGAAAGGA
  GTGGTAATGAAGGAGAGAGCAAGTCCTAAAGGAGAGGAAGCCAAGGAAGACCCTAAGGGCCACGACAGGA
  CACTGCCGCAGCAGCTGGGTGGCCAGAGCAGAGTGGCCCCCTCAGCCCACAGCTTCAACAATGATCTTGT
  GAAGGCCCTAGACCGAATCACACTGCAGAATGTTCCTTCGCAAGTAGCCTCGGGCTTGAACGCAGGAATG
  AGGAAGGACTTTGGTCTCCCTCTCACTGTCCTCTCATGCACGAAAGCTTGTCCTCACGTGGCTAAGTGTG
  GAAGTACTCTCTTTGAAGGGCGGACGGTACATCTTGGGAAGCTGTGTTGTACTGGAGTTGAAACGGAAGA
  TGACGAAGACACTGAGTCCCACTCATCAACAGAGCAGGCCCCCTCTGTTGAAGCCTCTGATGGACCAACA
  CTGCACGACCCAGACCTCTATATTGAGATTGTGAAAAATACGAAGTCCGTTCCAGAATACTCAGAGGTGG
  CTTATCCTGATTATTTTGGTCACATTCCACCTCCCTTCAAAGAGCCTATTTTAGAAAGGCCTTATGGTGT
  ACAAAGGACAAAAATTGCCCAAGACATCGAGAGGCTGATACACCAGAACGATATCATAGACCGCGTGGTG
  TATGACTTAGACAACCCTACCTATACCACTCCAGAAGAAGGAGATACTTTGAAGTTTAACTCAAAATTCG
  AATCTGGGAATCTGCGCAAAGTAATTCAAATTAGAAAAAGCGAGTACGACCTTATCCTGAACTCTGATAT
  AAACAGTAACCATTACCACCAGTGGTTCTACTTTGAAGTCAGTGGGATGCGGCCTGGTGTGGCATATAGG
  TTCAACATCATCAACTGTGAGAAGTCCAACAGTCAGTTTAATTATGGTATGCAGCCACTTATGTATTCAG
  TTCAGGAAGCACTAAATGCCAGACCATGGTGGATCCGTATGGGCACTGACATTTGTTACTACAAAAATCA
  CTTCTCACGAAGCTCAGTTGCCGCAGGCGGACAGAAGGGCAAGTCCTACTACACCATCACCTTCACCGTG
  AACTTCCCGCACAAGGACGATGTCTGCTATTTCGCCTATCACTATCCATACACGTACTCGACTCTGCAGA
  TGCATCTTCAAAAATTGGAATCGGCACACAATCCTCAACAAATCTATTTTCGAAAAGACGTGTTGTGTGA
  AACCTTGTCTGGAAACATCTGTCCTTTGGTGACCATAACAGCAATGCCAGAGTCCAATTACTATGAACAT
  ATCTGTCAGTTCAGAACTCGCCCTTATATTTTCTTGTCTGCTCGGGTCCATCCTGGAGAAACCAATGCAA
  GCTGGGTAATGAAAGGAACACTGGAGTACCTCATGAGCAATAGCCCGACTGCCCAGAGCCTACGGGAGTC
  TTACATTTTTAAAATTGTCCCCATGCTAAATCCAGATGGTGTCATCAATGGAAATCACCGCTGCTCCTTA
  AGTGGAGAGGATTTGAACAGACAGTGGCAAAGTCCAAACCCAGAGTTACACCCCACGATTTATCATGCCA
  AGGGGCTGCTGCAGTACCTGGCCGCGGTGAAGCGCCTACCTCTGGTTTATTGTGATTACCATGGCCATTC
  TCGAAAAAAGAATGTATTCATGTACGGCTGCAGCATCAAAGAGACGGTGTGGCACACCCATGACAACTCG
  GCTTCCTGTGATATTGTGGAAGACATGGGATACAGGACTTTGCCTAAGATACTGAGCCACATTGCTCCGG
  CATTTTGCATGAGCAGTTGTAGCTTTGTGGTGGAAAAATCTAAAGAATCCACAGCTCGGGTTGTCGTGTG
  GCGGGAAATTGGAGTTCAGAGGAGCTACACCATGGAGAGTACTTTATGTGGCTGCGATCAGGGTAGATAC
  AAGGGTTTACAGATTGGGACTCGAGAATTGGAAGAGATGGGAGCAAAATTTTGTGTTGGTTTATTGCGTT
  TGAAACGACTGACTTCTTCATTGGAATATAATCTGCCCTCCAACCTGCTTGACTTTGAAAATGACTTAAT
  TGAATCAAGCTGTAAAGTGACTAGCCCCACCACTTACGTTTTGGATGAAGATGAACCTCGGTTCCTTGAA
  GAAGTTGATTACAGTGCAGAAAGCAATGATGAGTTAGATGTTGAATTAGCGGAGAACACAGGTGATTATG
  AGCCTTCTGCCCAAGAAGAAGCCCTTTCTGACTCTGAGGTATCAAGAACACACCTGATTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001284219
• Insert Size: 2862 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001284219.1, NP_001271148.1
• RefSeq Size: 4488 bp
• RefSeq ORF: 2862 bp
• Locus ID: 67269
• UniProt ID: Q641K1
• Cytogenetics: 13
• Gene Summary: Metallocarboxypeptidase that mediates deglutamylation of target proteins. Catalyzes the deglutamylation of polyglutamate side chains generated by post-translational polyglutamylation in proteins such as tubulins. Also removes gene-encoded polyglutamates from the carboxy-terminus of target proteins such as MYLK. Acts as a long-chain deglutamylase and specifically shortens long polyglutamate chains, while it is not able to remove the branching point glutamate, a process catalyzed by AGBL5/CCP5. Deglutamylation plays a key role in cerebellar Purkinje cell differentiation, accumulation of tubulin polyglutamylation causing neurodegeneration.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) differs in its 5' UTR and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (3) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.