Tdrd5 (NM_001277730) Mouse Untagged Clone

CAT#: MC229200

Tdrd5 (untagged) - Mouse tudor domain containing 5 (Tdrd5), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tdrd5
• Synonyms: Gm103
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229200 representing NM_001277730
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGAACAAGAGCGTATACAGGACTGTCTGAGGAAAGAGATAAGGTCCCTTCTTATTTCCACTAAGG
  ATGGTTTGACTCCACAACAGTTGGAAAAGGAGTACCTTTTGATGGTTGGCAACCATCTCCCGCTCCGAAT
  CCTTGGATATCGGTCCACTATGGAACTGGTGTTGGATATGCCCGATGTGGTTAGTGTCTGCCCCGGTGGG
  GATGGTACCGTAATACTGAAAGCCATTCCAGACGAATCCACCAAAGGAATAGCAAGTTTGGTTGCAAAGC
  AGAGAAGGAGCCATAAGGTCCGAAACTCCATGCCAAAGGGAAGACCCAGCATATGCTCTGGCCGGGTGCC
  TTACCGAGGAAGGGTGCCCCCAATTCTTCCAGCTGTAGTGAAGAGATTCCCAGTAATGAAGGCACACTTT
  TCACAACCCATTTCAAATATGGAGCCCCCCAAACAAGTACTGAGCATGGCGAAGACACCCACGTTTAATT
  CTGTGGAGGCTTCAAGGCTGAACCACACTGAAAAATTAAATCAGTTGGAGAGCACATTCAAATCCGTGAT
  TGCACAGATTGGACCTGGAGGGACTGTTGATCCTGAGCTAAAGCGCAAGATACAATTTGTCGTGTCTAAG
  TTTCCACAAGGTTTGTTTATTTCTAAACTGCTTGGAGAATTTGAGCTAACTTTTAAAGAGCAACTTTCAC
  CAAAACAATTAGGGTTCTTAAACGTGACAGAACTTGTTGGCGCTCTTAGTGACATTCTCCGAGTTGAGTT
  CAGTGAAGAAAGGCAAGACTTGCTCGTGTTTGATGCTGACCTGAAGCCTCTGCCGTCTGGTGGACCGCTT
  TCATCAGTCAGAAATTCGTGTTTAGTTCAACCAGACAAGAAAACAGAAGCTAACCCTTGGACTTCCAGTC
  TCTCCAGAAATTCACTATCCACTGTAGCAGTTAAGAAGACCACGTGGGATTGCCCTTTGAAGAACCAGAA
  GGAGCCAGAACAGAAGATATACAAGAAGCCTAATCTGGTGGTCAAGCCTCTGCAGCTGCAGGTAGAAACA
  AACAAACCGCAGCTCAGCCTGTCAGTGGCAAACCACGATATCCCACCAGACGCTGTGCGTGCCAAGAAAT
  TGTGCAGACTTCCTCCATTAGATACCAGCACCCTTGTGGGGGTCTTCGTGGAGTATATAATCTCCCCTAG
  TCAGTTCTACATCCGGATCTACAGCAGAGATTCCTCAGAACTACTTGAAGACATGATGATTGAAATGCGA
  CGCTGCTACTCCAACCAGCTGGTCTCTGACCGGTACATTATGCCAGAATATTTTATACAACCAGGACATC
  TCTGTTGTGTAAAGATCTCTGAGGACAAGTGGTGGTACCGGGTTATTATCCATCGGATCCTTGGGAAAAA
  GGAAGTGGAAGTGTTCTACCCAGATTTCGGAAATATCGGAACTGTGCAGAAGTCCTCGCTGAGGTTCCTC
  AAATGCTGCTACACAAAGCTTCCAGCTCAGGCTATCCCTTGCTCTTTGGCGTGGGTGAGGCCCGCAGAGG
  AACACTGGACAGCCAGAGCTATTTTGCACTTCCAGAAGCTGTGTGGTTTGAAGCCATTGGTGGGGGTGGT
  GGATGAGTATATAGACGGAATCCTTAATATTTTTCTTTGTGATACGTCCTCAAATGAAGATGTGTATTTT
  CATCATGTCTTGAGGACAGAAGGCCATGCTATTGTATGCCGAGAAAATGCCCCATCCAAGGGCTTCAGGG
  ATTTCAACCCACCAGCTCTGTACACTAACGCCAGCTCAGCGGGCGACATGGTCCTGACAGACCTGGGGCA
  TCCTGCTCAGCAGCACTATTTAAATGAAGACCAGGAGATACTGCAGCAAGCCCAGCAAGATATTAATGAT
  GGAAAGTGTATAAGTTACCTTAAATCTGCGCCCAAGGAATTATTAAAGGATTCTAAATTGAGCTCATTGA
  AAACACATAAGAGCTGTGAAGAGGACCCACGGTGGTCCATCCTGCAGCCCAAGGATCTGAAGGAAGAAAA
  TGAGGATGAGGTCCCTACTGGAATGCCATGCCTGGAGTCAGTGACCATAGGTGACGATGTTTGGGATGAG
  AACTGGCTCCCTCTACAGGCCAAAATGGGGAAGGCAGGGAGTCCTGCCTCCCAGCTGTTTACTTCAAACC
  TTGTTGGGAAGAAACAATATCAGACAGGTGGAGAAATGGCACAAAAGGACTGGTGTTTTTCTACATCTAA
  AGATATATGGGATGATTCCTGGCAGCCCTTAGGCCTTGCGAATGATGTGAAAGGAGGAATTCACACACCA
  GAAGGGCCCATTGCTCAAGAAAAAAATACCAGCACAACCCGAATTCAACAGCAGCCAGATTTACAGTATC
  CTTTGGATTCTTCCACACTGCCCAAACTGGAAGAGTTCTACATCTCTCTGATCAAGTCTCAGCAGTCCGC
  AGAGAGGAGCCAGTCTGAACCTGCCAGCATTCAGACTCATGCCGGACGGGCAGCCTCAAAAGCACTCAGT
  TCAACCCCTGCAGTGGGTGACTCTCCAGAAAACCACTCTGGCTCTGTGGAAAGCTCTCCAGGGAGCCTAA
  AGAAAGAAGATGTTTCTAATAGCCGTGCTGAAGCCACGGCCAAGGACAAGAGTCAGGGAGCCATAGACCA
  GCTCTCTTTCATTTTGTCCCCCCAGCACCAGATTTCTCAGAAGCTCTACATCCCTCGAAGTACAGCCACA
  GCTGTCTTGGGAGCTGCGGCACGGTTAGCCACGTCCAGGAGCCTCCTGCACTGGTACCCCAGTGTGAAAG
  GAGGGAAGCTTGAGGCAGAGAGAGATGGAGTGAAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001277730
• Insert Size: 2838 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001277730.1, NP_001264659.1
• RefSeq Size: 3168 bp
• RefSeq ORF: 2838 bp
• Locus ID: 214575
• UniProt ID: Q5VCS6
• Cytogenetics: 1 G3
• Gene Summary: Required during spermiogenesis to participate in the repression transposable elements and prevent their mobilization, which is essential for the germline integrity. Probably acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Required for chromatoid body (CB) assembly.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR, and uses two alternate splice sites that result in the loss of an in-frame segment in the 5' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.