Pde2a (NM_001243758) Mouse Untagged Clone

CAT#: MC229199

Pde2a (untagged) - Mouse phosphodiesterase 2A, cGMP-stimulated (Pde2a), transcript variant 5

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pde2a
• Synonyms: CGS-PDE; cGSPDE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229199 representing NM_001243758
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTGGGGCTTGCACCCTTTTCAGCCTCCTGGGACGCCGATGAAGGCCTATCTGGTAGCCTTAGGA
  ACCCAGTGCTCTCACCCCACATTTGGGAACGGCGGGGCCAGCAGGTCTTCCTCAAGCCGGACGAGCCGCC
  GCCGCAGCCATGCGCCGACAGCCTGCAGGATGCTTTGCTGAGCCTAGGCGCCGTTATCGACATTGCTGGC
  CTGCGACAGGCTGCCAGGGATGCCCTCTCAGCCGTGCTCCCCAAAGTGGAGACTGTCTACACCTACCTGC
  TAGATGGGGAGTCCAGACTGGTGTGTGAGGACCCCCCTCATGAGCTGCCACAGGAAGGAAAAATTCGAGA
  AGCTGTGATCTCTCAGAAGCGGCTGAGTTGCAATGGGCTGGGACCTTCGGACCTACTGGGAAAGCCCTTG
  GCCAGGCTGGTGGCTCCACTGGCTCCTGACATGCAAGTGCTGGTCATACCCCTGCTGGACAAGGAGACTG
  GAAGTGTGGCAGCTGTCATCTTGGTGCACTGTGGCCAGCTGAGTGACAGTGAGGAACAGAGCTTGCAGGT
  GGTAGAGAAGCACGCTCTGGTAGCCCTGCGGAGGGTGCAGGCCCTGCAGCAGCGCAGGCCTGAAGCTGTT
  CAGAACACGTCAGTGGATGCCTCTGAAGATCAAAAGGATGAGAAGGGGTACACCGACCATGACCGAAAGA
  TCCTGCAACTGTGTGGGGAACTCTTTGACTTGGATGCTACTTCTCTGCAGCTCAAAGTCCTTCAATATCT
  GCAGCAGGAGACACAGGCCACTCACTGCTGCCTCCTGCTGGTGTCGGAGGACAATCTACAGCTTTCCTGC
  AAGGTCATTGGAGACAAAGTGCTGGGAGAAGAGGTCAGCTTTCCATTGACCATGGGACGTCTGGGGCAGG
  TGGTGGAAGACAAGCAGTGTATCCAGTTGAAGGACCTAACCTCTGACGATGTGCAACAGCTGCAAAACAT
  GTTGGGTTGTGAGCTGCAGGCTATGCTGTGTGTCCCTGTCATCAGCCGAGCCACTGACCAGGTGGTGGCC
  CTGGCTTGCGCCTTCAACAAGCTTGGAGGAGATTTCTTCACAGATGAGGATGAACACGTGATCCAACACT
  GCTTCCACTACACAGGCACGGTGCTCACCAGCACCTTGGCCTTCCAGAAGGAGCAGAAGCTCAAGTGTGA
  GTGCCAGGCTCTTCTCCAAGTGGCAAAGAACCTCTTCACCCACCTGGATGATGTCTCTGTCCTGCTACAG
  GAGATCATCACGGAAGCCAGAAACCTCAGCAACGCAGAGATCTGCTCGGTGTTCCTGCTGGATCAGAACG
  AGCTGGTGGCCAAGGTGTTCGATGGTGGCGTTGTGGACGATGAGAGTTATGAGATCCGCATCCCGGCGGA
  CCAAGGCATCGCGGGCCACGTGGCGACTACGGGCCAGATCCTGAACATCCCAGATGCATATGCCCATCCG
  CTTTTCTATCGCGGCGTAGATGACAGCACTGGCTTCCGCACGCGCAACATTCTCTGCTTCCCTATCAAGA
  ACGAGAACCAGGAGGTCATTGGTGTGGCTGAGCTAGTGAACAAGATCAATGGGCCATGGTTCAGCAAGTT
  TGATGAGGACCTGGCCACAGCCTTCTCCATCTACTGTGGCATCAGCATCGCCCACTCTCTCCTATACAAA
  AAGGTGAATGAAGCCCAATACCGCAGCCACCTGGCCAATGAGATGATGATGTATCATATGAAGGTCTCTG
  ATGATGAATACACCAAGCTTCTCCACGATGGCATCCAACCTGTGGCCGCCATTGACTCCAACTTTGCCAA
  CTTTACCTACACGCCTCGGTCTCTGCCTGAGGACGACACTTCTATGGCCATCCTGAGCATGCTGCAAGAC
  ATGAACTTCATCAATAACTACAAAATTGACTGCCCAACTCTGGCCCGATTCTGCCTGATGGTGAAGAAAG
  GCTACCGGGATCCACCCTACCACAACTGGATGCACGCCTTCTCTGTCTCTCATTTTTGCTACCTGCTCTA
  CAAGAATCTGGAGCTCTCCAACTACCTCGAGGACATCGAGATCTTTGCATTGTTTATTTCCTGCATGTGT
  CATGACCTGGACCACAGAGGCACAAACAACTCCTTCCAGGTGGCCTCGAAATCTGTGCTGGCCGCACTCT
  ACAGCTCAGAGGGCTCTGTCATGGAGAGGCACCACTTTGCTCAAGCCATTGCTATCCTCAACACCCACGG
  CTGCAATATCTTTGACCACTTCTCTCGGAAGGACTATCAGCGCATGCTGGACCTGATGAGGGACATCATC
  TTGGCTACAGACCTGGCACACCACCTCCGCATCTTCAAGGACCTGCAGAAGATGGCTGAAGTGGGTTATG
  ACCGAAACAACAGGCAACACCACAGGCTTCTTCTGTGCCTCCTCATGACCTCCTGTGACCTCTCTGACCA
  GACAAAGGGCTGGAAGACCACCAGAAAGATTGCAGAGCTGATCTACAAAGAGTTCTTCTCCCAGGGAGAC
  TTGGAGAAGGCCATGGGCAACCGACCGATGGAGATGATGGACCGTGAGAAGGCCTACATCCCTGAGCTTC
  AGATCAGCTTTATGGAGCACATTGCCATGCCTATCTACAAGCTTTTACAAGACCTGTTCCCCAAGGCGGC
  AGAGCTGTATGAACGTGTGGCCTCCAACCGTGAGCACTGGACCAAGGTGTCCCACAAGTTCACCATCCGA
  GGCCTCCCCAGTAACAACTCGCTGGATTTCCTGGACGAGGAATACGAGGTCCCCGATTTGGACGGCACCA
  GAGCTCCTGTCAATGGCTGCTGCAGCCTCGAGGGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001243758
• Insert Size: 2838 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001243758.1, NP_001230687.1
• RefSeq Size: 4112 bp
• RefSeq ORF: 2838 bp
• Locus ID: 207728
• Cytogenetics: 7 E2
• Gene Summary: Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (5) differs in the 5' UTR, uses an alternate splice site in the coding region and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (5) is longer and has a distinct N-terminus, compared to isoform 6. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.