Mcf2 (NM_001289730) Mouse Untagged Clone

CAT#: MC229197

Mcf2 (untagged) - Mouse mcf.2 transforming sequence (Mcf2), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mcf2
• Synonyms: B230117G22Rik; Dbl; Mcf-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229197 representing NM_001289730
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAAGCAAATCCCCCGAGAGGCAAGATGAGGTTCAGAAGGAATGCGGCCTCCTTCCCTGGGAGCT
  TACACTTAGTCCTGGTTTTGCGTCCTACCAGTTTTCTTCAACGGACTTTCACGGACATTGGATTTCGATT
  TAGCCAGGAGGATTTTATGCTGAAATTACCAGTTGTTATGCTGAGCTCAGTTAGTGATTTGCTGACATAC
  ATTGATGACAAGCAGTTAACCCCTGAGTTAGGCGGCACCTTGCAGTACTGCCACAGTGAATGGATCATCT
  TCAGAAATGCCATAGAGAAGTTTGCTGTCACAGTGAAAGAAATGGCTCAGATGTTGCAATCCTTTGGAAC
  AGAATTGGCTGAAACAGAGCTACCACAGGATATCCTTTCAATAGAAGAGATTCTGGCAGGTCGAGCCGAA
  AGGTATCACCTGTTGAAGAATGACTTAACTGCTGTAACCAAAGAAGGAAAAGTTCTGCTCATGAACCTGC
  AAGTGCCTGCCACTGAAGAAACTGTCAGTTCAAGCCTTGAATGTACTCAGCACATAAATGGCGACTGGCA
  AACTATTAAAAAGTTGCTGGCTCAGGTGCATGATATGGAAACTGCTTTTGATGGATTTTGGGAAAAACAT
  CAGCTGAAAATGGAGCAGTATCTGCAACTGTGGAAGTTTGAACAAGACTTTCAAGAGGCTGTGACTCAAG
  TTGAATTTTTATTAAGTCAACAAAGAGAACTGGGTGATATAACAGGGAACTTAGCTCAAGTAAAACAAAG
  ACTTAAAAAACTAGAGATCTTAGATGATAAATCTCAGGAACTGTTAACCAAGGCCCGGATTGTGATACTA
  CGTGGCCACAAGCTGGCATCGAATCACCACTATGCACTTGATTTAATTTGCCAGCGATGCAATGAACTAC
  GCTACCTTTCTGATATTTTAGTTAATGAGATCAGAACAAAGCGGGTCCAACTGAGCAGGACTTTCAAAGT
  GCATAGACTCTTACAGCAGGCACGTCAGTGCTGTGATCAAGGGGAATGCCTCCTGGCTAGCCAAGGAATG
  GATAAGTTTCAGACTAAAGAAGATGCTCAGAAAGCCCTTCAGGATGTGGACAATTTCCTTCAAATGGCCA
  TGCCTTTCATCAATTATGATATCGAAAGCCTGCAATATGAATTTGATGTACTCCTATCTCCTGAACTCAA
  GGCTCAAATGCAGAATATACAACTAAAGCTTGAAAATATTCGAAGTGCATTTCAGAACCAACAGGCTGGT
  TGTAAGAGCCTAAAAGAAGTACCCGAGGGGGCGTTCCAGAACTTGGTTCCTGCATCTGAAAATGTGATGC
  GGTCCAGAATGATATTCTTTTCACCGAAACATGTGGGAATTGGATATTCTTTCTTTCAAGCATGTAGAAT
  TTTTTTAAAAGTGAAAAAGTCTTGGAGACAGATTCACGCTCAGAGCAATGTGAAAGTTGAAGCCGTTGAG
  GATAGCCAAGAGAAGAATTCTGATCAGTCTCCCAAATTGGACAATAGCTTGGATATTTTAAAGAACCATG
  TCCTAAATGAGCTGATACAGACAGAGAGAGCATATGTTCGAGAGCTGTTTACTGTTTTGTTGGGCTACAG
  ATCTGAGATGGACAATCCACAGATGTTTGATCTTATGCCACCTCTCCTGAGAAATAAAAAGGATGTTCTC
  TTTGGAAATATGGCAGAAATATACGAATTCCATAACAACATTTTCATGAGCAGACTGGAAGATTGCTCTG
  ATGCTCCAGAAAGAGTGGGACCTTGCTTCCTGGAAAGGAAGGATGATTTTCAGATGTATGCCAAATATTG
  TCAGAATAAACCCAGGTCAGAGTTAATTTGGAGGAAGTATTCTGAATGTGCCTTTTTCCAGGAATGTCAA
  CGAAAATTGAAACATCGACTTGGTCTGGATTCCTATTTGCTCAAACCTGTTCAACGAATCACTAAGTATC
  AGTTGCTTTTAAAGGAGCTTTTAAAGTATAGCAAAGAAGGTGAAGGAACTACTAAATTAAAGGAGGCACT
  TGACTCAATGCTGGAGCTGCTGAAGTCAGTGAATGACTCCATGCATCAGACTGCCATCAATGGCTATGTT
  GGAAATATAAATGAGCTGGGCAAGATGGTTCTTCAAGGTTCCTTCAATGTTTGGCTAGGACACCGGAAAG
  GTGCTACAAAAATGAAGGATTTTGCACGTTTCAAACCCATGCAGAGACATCTTTTCTTATATGAAAAAGC
  TGTTATGTTTTGTAAGAGGCGTTTCGAATCTGGAGAAGGTGCTGATAGATACCCATCATACAGTTTTAAG
  CACTGCCTAAAAATGGAAGATGTTGGCATCACAGAACATGTGAAAGGTGATAACCGCAAATTTGAAATAC
  GGTATAGTGAGAAGGAAGAAATTTACATTGTTCAGGCTCCAAATGTTGATGTGAAGATGTTATGGCTAAA
  AGAAATAAGAAAAATTTTGGTGCAGCAGAAGGAACTTATGACTGCTAAAACCCAGCAGGATCAGGCACTA
  GACCAGGACCAACTTTTCCCCCAGCAGCAGAATGCCGAGCTATGCAAAAGCTCACCTTTTTGTGTGTGTG
  AGGAAACTCTTTTCAACGCCACAGAAGCAGGAGCAGAAGTGGAGCAAGCTGGTGCCCTGATAAAGGTCAG
  GGAGGCAGCTGTGCTAGCTCAAGCAGAGGCAAGTTCAGCAGCTTGGAATGGGATGTTACCATCTGCAGAA
  GGCGCTGCTGCAATTGCTGAACATTCATACAATTATTTCTCCTCTAACAATGACCATGGAGAAGATAGGA
  CACAAATGAGACACATGTCGGAGGTGACATTCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289730
• Insert Size: 2835 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289730.1, NP_001276659.1
• RefSeq Size: 3719 bp
• RefSeq ORF: 2835 bp
• Locus ID: 109904
• Cytogenetics: X 33.5 cM