Hk3 (NM_001206390) Mouse Untagged Clone

CAT#: MC229178

Hk3 (untagged) - Mouse hexokinase 3 (Hk3), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hk3
• Synonyms: HK-III; HK III
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229178 representing NM_001206390
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACCATTGGGCCTTCTGGCCTGCATCCTGGAGAAAGAGCCTCAGTCTGCCCACACGAGGGCGTGC
  CAAGGCCCTCAGGTAGCTTAGAACTGGAATGCTTGCAGCAATTCAAGGTGACCAGGACACAGTTGCAGCA
  GATCCAAGCCAGTCTCCTGTGCTCCATGGAGCAGGCACTGAAGGGACAGGACAGTCCCGCTCCTTCTGTC
  CGGATGCTGCCCACATACGTGAGGTCCACACCACACGGCACCGAGCAAGGGGATTTCCTGGTGCTGGAGC
  TGGGGGCCACAGGAGCATCACTACGTGTGTTGTGGGTAACACTGACAGGCACCAAAGAATGCCGGGTGGA
  GCCCAGGAGCCGGGAGTTTGTGATTCCTCAAGAGGTGATCCTAGGTGCTGGCCAGCAGCTCTTTGACTTT
  GCTGCCCGATGCCTCTCTGAATTCCTGGATGCATACCCCGTGGAGAATCAGGGTCTGAAGCTTGGGTTCA
  ATTTCTCTTTTCCTTGTCACCAGACAGGCTTGGACAGGAGCACCCTCATTTCCTGGACAAAAGGTTTTAG
  GTGCAGCGGCGTGGAAGGCCAGGATGTGGTCCAGTTGCTAAGAGATGCCATTCAGAGGCAGGGGACCTAC
  AGGATTGATGTGGTAGCCATGGTGAATGACACGGTGGGCACCATGATGGGCTGTGAGCTGGGCACCAGGC
  CGTGTGAAGTCGGGCTGATTGTAGACACCGGTACCAACGCGTGCTACATGGAGGAGGCGCGGCACGTGGC
  CGCTCTGGATGAGGACCGCGGCCGCACCTGTGTCAGCATCGAGTGGGGCTCCTTCTATGACGAAGATGCT
  CTAGGGCCAGTACTGACCACCTTCGACTCTGCCCTGGACCGCGAGTCCCTGACTCCTGGTGCTCAAAGGT
  TTGAGAAGATGATTGGAGGCCTTTACCTGGGTGAGCTGGTGAGGCTGGTTCTGGTCCACTTGACCCAGCA
  CGGGGTCCTCTTTGATGGCTGCGCCTCTCCTGCGTTGCTGAGTCAAGGCTGTATCCTCCTGGACCACGTG
  GCTGAGATGGAGGACACCGCCACTGGGACAGCCCGCGTCCACACCATCCTGCAGGACTTGGGTCTGAGCC
  CTCGGGCCTCAGATGCTGAACTGGTGCAATATGTGTGCGTGGCTGTGTGCACACGGGCTGCCCAGCTCTG
  TGCGGCTGCCCTGGCTGCAGTCCTCTCCCGCCTCCAGCACAGCAGGGAGCAGCAGACATTGCAAGTGGCT
  GTGGCCACTGGAGGGCGAGTGTTTGAACGACACCCCAGGTTCCTCCGAATCCTAAAGGAGACGGTAACAC
  TCCTGGCCCCAAACTGTGATGTCTCCTTCATCCCCTCTGTGGATGGTGGTGGCCGGGGTGTGGCAATGGT
  GACAGCTGTGGCTGCCCGCCTGGCTGCTCACAGGCGCATCCTGGAAGAGACCCTGGCACCATTTCAGCTG
  ACCTTGGAGCAGATGACAGTGGTGCAGGCACAAATGCGGGAAGCCATGATCAGGGGGCTTCAAGGAGAGG
  CCTCCTCCCTCCGCATGCTGCCCACTTACGTCCGAGCGACGCCCGACGGCAGCGAGCGAGGTGATTTCCT
  GGCCTTGGACCTAGGGGGCACCAACTTCCGGGTCCTGTTGGTACGAGTGGCGGAGGGCAGTGTGCAGATC
  ATCAACCAGGTCTACTCCATTCCTGAGTGTAGAGCCCAGGGCTCTGGACAGAAGCTCTTTGACCATATTG
  TGGACTGCATCGTGGACTTCCAGAAGAGGCAGGGCCTTAGTGGACAGAGCCTACCCCTGGGTTTCACCTT
  CTCTTTCCCATGCAAGCAGCTTGGCCTGGACCAGGGCATCCTCCTGAATTGGACTAAGGGGTTCAATGCA
  TCAGGCTGTGAGGGCCAGGATGTTGTGTATCTATTACGGGAAGCCATTAGGCGCAGACAGGCAGTGGAGC
  TGAATGTGGTTGCCATTGTCAATGACACGGTGGGGACCATGATGTCCTGTGGCTATGATGATCCCCGTTG
  TGAGATGGGCCTCATTGTCGGAACCGGCACCAACGCCTGTTACATGGAAGAGCTCCGCAATGTGGCAAGT
  GTGCCTGGGGACTCGGGCCTCATGTGCATCAACATGGAGTGGGGTGCCTTTGGGGATGATGGCTCACTGG
  GCACACTCAGCACCCGCTTTGACACCAGTGTGGACCAGGCATCCATCAATCCAGGCAAACAGAGGTTTGA
  GAAAATGATCAGCGGTATGTACTTGGGGGAGATCGTCCGCCATATCCTCCTGCACTTAACCAATCTCGGA
  GTTCTCTTCCGAGGCCAGAAGACTCAATGCCTTCAGGCCAGGGACATCTTCAAGACTAAGTTCCTCTCTG
  AGATCGAAAGTGATAGCCTGGCCCTGCGTCAGGTCCGAGCCATCCTGGAAGATCTGGGGCTGACTCTGAC
  ATCTGATGACGCCTTGATGGTCCTGGAAGTGTGCCAGGCTGTGTCTCGCAGGGCTGCCCAACTCTGTGGG
  GCAGGTGTGGCTGCCGTGGTGGAGAAGATTCGGGAGAACCGGGGCCTGCAGGAGCTGACAGTGTCTGTGG
  GAGTAGATGGGACACTCTACAAGCTACATCCTCACTTCTCCAAGCTGGTGTCGGCAACAGTCCGGAAGCT
  AGCCCCTCAGTGCACCGTCACCTTTTTGCAGTCGGAGGATGGGTCTGGCAAAGGCGCAGCTTTGGTTACT
  GCTGTTGCTTGTCGCCTTACCCAGATGGCCCACGTCTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001206390
• Insert Size: 2769 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001206390.1, NP_001193319.1
• RefSeq Size: 3029 bp
• RefSeq ORF: 2769 bp
• Locus ID: 212032
• UniProt ID: Q3TRM8
• Cytogenetics: 13 B1
• Gene Summary: Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D-fructose 6-phosphate, respectively). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.