Mib2 (NM_001256108) Mouse Untagged Clone

CAT#: MC229177

Mib2 (untagged) - Mouse mindbomb homolog 2 (Drosophila) (Mib2), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mib2
• Synonyms: 2210008I11Rik; skd; Zzank1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229177 representing NM_001256108
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCTGGACCCCCATGCAGGTGTGCAGGTGGGCATGCGTGTGGTACGCGGAATGGACTGGAAATGGG
  GCCAGCAGGATGGAGGTGAGGGCGGTGTGGGCACCGTGGTGGAGCTTGGCCGCCATGGCAGCCCCTCGAC
  CCCCGACCGTACAGTTGTCGTTCAGTGGGACCAGGGCACACGTACCAACTATCGAGCTGGCTACCAAGGT
  GCCCATGACCTGTTGCTCTATGACAACGCCCAAATCGGTATCCGCCACCCCAATATCATCTGTGACTGCT
  GCAAGAAACATGGGCTTCGGGGCATGCGTTGGAAGTGCCGCGTCTGCTTTGACTATGACCTCTGCACACA
  GTGCTACATGCACAACAAGCATGACCTGACCCATGCCTTCGAGCGCTATGAGACATCTCACTCACGCCCG
  GTTACGCTGAGCCCCCGACAGGGCCTCCCTCGGATCCCACTGAGGGGCATCTTTCAAGGAGCTAAGGTGG
  TACGAGGCCCTGACTGGGAATGGGGCTCACAAGATGGAGGGGAAGGCAAGACAGGTCGTGTGGTGGACAT
  CCGTGGCTGGGATGTGGAGACAGGCCGAAGTGTGGCCAGTGTGACGTGGGCAGATGGAACCACGAACGTG
  TACCGAGTGGGCCACAAGGGCAAGGTGGATCTCAGATGTGTTGGTGAGGCAGCTGGCGGCTTTTACTACA
  AGGAGCACCTCCCAAAGCTTGGCAAGCCAGCAGAGCTGCAGCGCAGGGTGAGTGCTGATGGCCAGCCCTT
  CCAGCGTGGGGACAAGGTCAAGTGTCTGCTGGACACAGATGTCCTAAGGGATATGCAAGAAGGCCATGGT
  GGCTGGAACCCTAGGATGGCAGAGCACAACAGCTTCTGGGTGGGTGATGTGGTCCGGGTCATCGGTGACC
  TTGACACTGTGAAGCGACTTCAGGCCGGACATGGTGAATGGACCGACGACATGGCTCCCGCCCTGGGCCG
  AGTGGGGAAAGTGGTGAAGGTGTTTGGAGATGGAAACTTGCGTGTGGCGGTTGGCGGTCAGCGGTGGACC
  TTCAGCCCCTCTTGCCTAGTGGCCTACCGGCCTGAGGAAGACGCCAACCTGGATGTGGCTGAGCGTGCCA
  GAGAGAACAAAAGCTCACTGAGTGTGGCCCTGGACAAGCTTCGGACCCAGAAGAGTGACCCAGAGCACCC
  GGGGAGGCTGGTAGTAGAGGCCGCACTAGGAAATGTAGCCCGGGCTCTAGATCTTCTGCGGAGGCATCCA
  GAGCAGGTGGACACCAAGAACCAGGGTAGAACTGCCCTGCAGGTGGCTGCTTACCTGGGCCAGGTAGAGC
  TGGTGCGGCTGCTGCTGCAGGCAAGAGCAAGTATGGACCTGCCGGATGATGAGGGCAACACTGTGCTGCA
  CTACACAGCCATGGGGAACCAGCCTGAGGCCACAAGGGTGCTCCTGAGTGCAGGGTGTGCAGTGGATGCT
  CGGAATGGCACACGCAGCACAGCCCTACATGTGGCTGTACAGAGGGGCTTCCTAGAGGTGGTAAAGATCC
  TGTGTGAACGTGGTTGTGATGTCAACTTGCCGGATGCGCATGCAGACACACCCCTGCATTCTGCCATCTC
  TGCTGGTGCTGGTGCCAGCAGCATTGTTGAAGTCCTCACCGAGGTCCCTGGCATCGATGTCACTGCTACC
  AATAGCCAGGGCTTCACACTGCTACACCATGCATCCCTCAAGGGCCATGTGCTAGCAGTCAGGAAGATTC
  TGGCAAGGGCCCGGCAGCTGGTGGATGCCAAGAAGGAGGATGGCTTTACCGCACTGCATCTGGCAGCCCT
  CAACAACCACCGGGAGGTGGCCCAGGTCCTAATCCGAGAGGGTCGCTGTGATGTGAATGTGCGGAACCGG
  AAGCTTCAGTCTCCACTTCATCTGGCTGTACAGCAGGCCCACCTGGGGCTGGTACCGCTGCTAGTGGACG
  CAGGCTGCAGTGTCAACACGGAGGACGAGGAGGGTGACACGGCCCTGCATGTGGCACTACAGCGTCACCA
  GCTGTTGCCCCTTGTGGCTGACAGGGCTGGGGGAGACCCAGGGCCCTTGCAGCTGCTGTCAAGGCTACAG
  GCCTCAGGCCTCCCAGGCTGCACAGAGTTGACTGTAGGCGCTGCAGTGGCCTGCTTCCTGGCGTTGGAGG
  GTGCTGATGTGAGCTACGCAAACCACCGCGGCCGGAGCCCACTGGACCTGGCCACAGAGGGCCGAGTGCT
  CAAGGCCTTGCAGGGCTGTGCCCAGCGCTTCAGGGAGCGACAGGCAGGTGGCGGTGGGGGTGTGCCCCCG
  GGCCCCCGGCATGTGCTGAGTACTCCTAACACCGTGACGAACCTGCACGTGTCTGGCACGGCAGGGCCAG
  AAGCTGCCGAGTGCCTGGTGTGCTCGGAGCTGGCATTGCTAATTCTGTTCTCACCGTGTCAGCACCGCAC
  AGTGTGTGAGGAGTGCGCTCGAAGGATGAAGAAGTGTATCAGGTGCCAGGTGGTCATCAGCAAGAAGCTC
  CGCCCAGACGGCTCAGAGGTGGTAAATGCCATCCAGGTGCCCGGCCCGCCTCGGCAACTGGTGGAGGAGC
  TACAGAGCCGCTACAGGCAGATGGAGGAGCGCATCACCTGCCCCATCTGCATCGACAGCCACATCCGCCT
  GGTGTTCCAGTGCGGCCATGGAGCGTGCGCGCCCTGTGGCGCCGCGCTTAACGCCTGCCCCATCTGCCGC
  CAGCCCATCCGCGACCGCATTCAGATCTTCGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001256108
• Insert Size: 2766 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001256108.2, NP_001243037.2
• RefSeq Size: 3827 bp
• RefSeq ORF: 2766 bp
• Locus ID: 76580
• UniProt ID: Q8R516
• Cytogenetics: 4 E2
• Gene Summary: E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR and lacks an in-frame exon in the 5' coding region compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 1. Variants 2, 3, 5, and 6 all encode the same isoform (2).