Tubgcp2 (NM_001286007) Mouse Untagged Clone

CAT#: MC229148

Tubgcp2 (untagged) - Mouse tubulin, gamma complex associated protein 2 (Tubgcp2), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tubgcp2
• Synonyms: 1700022B05Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229148 representing NM_001286007
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGAATTTCGGATTCACCATGATGTCAATGAGCTGCTCAGCCTGCTCCGCATCCATGGCGGGGATG
  GGGCTGAGGTCTACATTGACCTGCTGCAGAAGAACCGGACGCCGTATGTCACAACCACAGTCTCTGCCCA
  CAGTGCCAAGGTCAAAATTGCAGAATTTTCTCGAACTCCTGAGGACTTTTTAAAGAAATATGATGAACTG
  AAATCTAAAAATACAAGGAACCTTGACCCACTGGTGTACCTGTTATCAAAACTCACAGAAGACAAAGAGA
  CACTTCAGTTTTTGCAACAGAATGCAAAGGAAAGAGCTGAGCTTGCAGCTAGTGCTGCAACTAGCAACAC
  CACCAGCTTCAGCATCCCTGTTGCAGCCTCTAAGATGTCCACACAAGAGCTAGAGGAACTGAGGAAGCAG
  CTTGGCAGTGTATCCACAGGCTCCACACTGCAGCAGTCTCTGGAACTTACAAGAAAGATGCTTCGAGACA
  AACAGAACAAAAAAAATTCAGGCCAGCCCCTCCCTGTCTTCCCAGCATGGGTGTATGAGAGACCAACATT
  GGCTGGAGATTTCCTGATTGGCTCGGGCTTGAGCTCAGACACAGTTTTGCCTATAGGTACATTGCCCTTG
  GCCTCCCAGGAGTCAGCTGTGGTGGAAGATCTGCTCTATGTGCTTGTGGGTGTGGATGGCAGGTATATCA
  CTGCTCAGCCTTTGGCTGGGAGGCAGAACCGTACCTTCCTTGTCGACCCCAACCTGGACCTGTCCATCCG
  GGAGCTAGTAAACAGAATCCTCCCTGTGGCCGCCAGCTACTCTACTGTGACCAGGTTCATTGAGGAGAAG
  TCTTCATTTGAATATGGGCAGGTGAACCATGCCCTGGCTGCTGCCATGAGAACCCTAGTAAAGGAATACC
  TGATTCTGGTCACACAGCTGGAACAGCTGCACAGGCAGGGCCTCCTCTCACTGCAGAAGCTCTGGTTCTA
  CATCCAGCCAGCCATGCGCACCATCGACATCCTTGCCTCCCTTGCCACCTCAGTGGATAAAGGCGAGTGT
  GTGGGCGGGTCGACACTGAGCCTTCTTCATGATAGGAGCTTCAACTACACCGGGGACAGCCAGGCGCAGG
  AGCTGTGCCTCTATCTGACCAAGGCAGCCAGTGCACCTTACTTCGAGATCCTGGAGAAGTGGATCTATAG
  GGGCATCATTCATGACCCATACAGTGAGTTCATGGTGGAGGAACACGAGCTGCGGAAGGAGAAGATCCAG
  GAGGACTACAACGACAAGTACTGGGACCAGCGCTACACTGTGCTGCCGCAGCAGATCCCATCCTTCCTGC
  AGAAGGTAGCAGGCAAGATCCTCAGCACAGGAAAATACCTAAATGTGGTCAGAGAGTGTGGTCATGATGT
  CACCTGTCCCGTAGCCAAAGAAATCATCTATACCCTTAAAGAGCGGGCATATGTGGAGCAGATAGAGAAA
  GCGTTCAATTATGCCAGCAAAGTGCTGCTGGACTTCCTCATGGAGGAGAAGGAGCTGGTGGCCCACCTGA
  GGTCCATCAAGCGCTACTTCCTGATGGACCAGGGGGACTTCTTCGTGCATTTCATGGACCTGACTGAGGA
  GGAGCTCAGGAAGCCAGTGGAGGACATCATACTCACACGCCTGGAGGCCCTGTTGGAGCTGGCCCTGCGA
  ATGAGCACTGCCAACACTGACCCCTTCAAAGACGACCTCAAGATTGAACTGATGCCTCACGACCTAATCA
  CTCAGCTTTTGCGGGTTCTGGCCATCGAGACCAAGCAGGAAAAGGCAATGACCCATGCTGACCCCACGGA
  GCTCACACTGAGTGGTCTGGAGGCCTTCTCCTTTGACTACATGGTCAAATGGCCCCTGTCACTGATTATT
  AACAGGAAGGCACTCACCCGCTACCAGATGCTCTTCCGACACATGTTCTACTGCAAGCACGTGGAGCGGC
  AGCTCTGCAGTGTCTGGATCAGCAACAAGACGGCCAAGCAGCATGCACTGCACTCCGCCAAGTGGTTTGC
  TGGTGCTTTCACTCTGCGGCAGCGAATGCTCAACTTTGTCCAGAATATTCAGTACTACATGATGTTTGAA
  GTGATGGAACCGACCTGGCACATCCTTGAGAAAAACCTGAAATCTGCCTCCAACATTGACGATGTCCTTG
  GCCACCACACAAGCTTCCTTGACAACTGCCTGAAGGACTGCATGCTGACCAACCCTGAGCTGCTGAAGGT
  TTTCTCCAAGCTCATGTCCGTATGCGTCATGTTTACCAACTGTATGCAGAAATTTACACAGAGTATGAAA
  CTGGACAGTGAGCTAGGCCGTCTGACACTGGATCAGGGTAGTGTGCAGGGGCCACCCACAGAGACTGAAC
  GGATTGAGGATCGCCCCCGGAAGGAGCTTACCAGGAAGCATCTGTCTGAGCATGTGGATGCTCCTCAGCT
  GGCCTCTGGTTTCGAGGCCACCATCAACAACTTTGACAAAAACTTCTCTGCCCATCTTCTTGATTTGCTG
  GCCCGACTAAGTGTCTACAGCACCAGTGACTGCGAACATGGCATGGCCAGTGTCATCTCCAGACTAGATT
  TCAATGGTTTCTATGCTGAACGCCTGGAACGTCTGTCTGCAGAAAGGAGTCAGAAGGCTGCCCCCCAAGT
  GCCTGTCCCACGGGGACCCTCAGCACCAGCACCCAGGGTGGCCATTCCTGCGCAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001286007
• Insert Size: 2718 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286007.1, NP_001272936.1
• RefSeq Size: 3158 bp
• RefSeq ORF: 2718 bp
• Locus ID: 74237
• UniProt ID: Q921G8
• Cytogenetics: 7 F4
• Gene Summary: Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Variants 1 and 2 both encode the same isoform (a).