Limch1 (NM_001256122) Mouse Untagged Clone

CAT#: MC229141

Limch1 (untagged) - Mouse LIM and calponin homology domains 1 (Limch1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Limch1
• Synonyms: 3732412D22Rik; mKIAA1102
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229141 representing NM_001256122
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCCTGAAAGACAGGAGACTGACGACATTGATAGCCCGAAGCGCAGCATCCGAGACAGTGGCTACA
  TCGACTGCTGGGACTCTGAACGCAGCGACTCCCTCTCTCCTCCTCGCCACGGACGGGATGACTCCTTTGA
  CAGCCTGGATTCTTTTGGCTCCCGCTCCAGGCAGACACCTTCGCCAGATGTAGTTCTCAGGGGAAGCAGT
  GATGGAAGAGGAAGCGATTCTGAATCAGACTTGCCGCACCGGAAGTTGCCAGATGTGAAGAAAGATGATA
  TGTCCGCACGAAGGACTTCTCATGGTGAGCCGAAATCAGCAGTGCCTTTTAACCAGTACCTCCCGAACAA
  AAGCAACCAGACGGCCTACGTCCCGGCGCCTCTGAGAAAGAAGAAGGCGGAGCGGGAGGAATTCAGGAAG
  AGTTGGAGCACCGCCACCTCCCCCCTGGGTGGGGAAAGGCCCTTCAGATACGGTCCCAGAACTCCTGTGT
  CAGATGACGCAGAGAGCACGAGTATGTTTGACATGCGGTGTGAGGAGGAGGCAGCGGTGCTGCCGCACAG
  CAGGGCCCGCCAGGAGCAGCTGCAGCTGATAAATAACCAGCTGAGGGAAGAGGACGACAAATGGCAAGAT
  GACCTGGCTCGCTGGAAGAGCCGGAGAAGAAGCGCTTCTCAGGACTTGATCAAGAAAGAGGAAGAAAGGA
  AAAAAATGGAGAAGCTGATGTCTGGGGAAGATGGGACAAGTGAGCGGAGGAAAAGCATCAAAACTTACAG
  AGAAATCGTCCAAGAAAAAGAGCGGAGAGAGAGGGAACTTCATGAGGCGTACAAGAACGCAAGGTCGCAG
  GAGGAGGCCGAGGGGATCCTCCAGCAGTACATCGAGAGGTTCACCATCAGTGAGGCTGTCCTCGAACGCT
  TGGAGATGCCAAAAATCCTGGAAAGAAGCCATTCAACAGAGCCAAACGTGTCCTCCTTCCCAAACGACCC
  CAGCCCCATGAAGTACCTGCGGCAGCAGTCACTGCCCCCACCCAAGTTCACTGCCACGGTTGAGACCACC
  ATAGCTCGCACCAGTGTCCCAGAGAGCATTGCGTCAGCAGGCACTGGGTCTCCAAGCAAAATCATCACTC
  CAAACACAGTGCCTATGCTGACACCCAGGCCTTACTCCCAGCCCAAAAACTCTCAAGAGGTTCTGAAGAC
  TTTTAAGGTAGATGGGAAGGTCAGCATGAATGGGGAAATGGCCCCTGGAGATGAGGAAGGAAAGGAAAAA
  GAAGGCCCTGCAGCTGCAGCCCCTGGCCCTTCCTTAACCAAGTCCCAGATGTTTGAAGGTGTGGCCACGG
  TGCACGACTCTCCTGTGCAGGTGAAACAAGGCAGCAACAGCATCGAGATCAACATCAAGAAGCCAAATTC
  TGCTCCCCAGGAACTGACAGCAGCCTCTGAGGAAACTGAATCGAACGGCCAAGAGGATGAAGACGGTGAA
  GAAAGGCCAGGGACAGGGGATTTGGAGCCTGACTCGGCAGAACCCCAGCACTTTACGACAACTGTGACTC
  GGTGCAGCCCAACAGTGGCCTTGGTGGAGTTTTCATCCAACCCGCAGCTGAAGAATGAGGTGCCAGAACA
  AGGCCAGAAGAAGCCAGAAGATGAGATGAGTGGGAAGGTGGAGTTAGTGTTGTCACAGAAGGTGGCAAAG
  CCAAAATCCCCAGAGCCTGAAGCAACCCTGACGTTTCCATTTCTCGACAAAATGCCTGAAACCAACCAAC
  TACATTTGCCAAATCCCAGTTCTCAAGCGGATTCTCCAAGCAGTGAAAAGTCCCCTGGGAGCACACCCTT
  TAAGTTCTGGGCATGGGACCCAGAAGAGGAGCGCCGGCGACAGGAGAAATGGCAGCAGGAGCAGGAACGT
  CTGCTGCAGGAGAGGTATCAGAAGGAGCAAGACAAGCTGAAGGAGGAGTGGGAAAAGGCCCAGAAGGAGG
  TCGAAGAAGAAGAACGCAGATACTACGAGGAGGAGCGTAAGATAATTGAAGACACGGTGGTTCCATTCAC
  TATTTCCTCAAGCTCTGCAGACCAACTGTCTACGTCCTTGTCTGTAACTGAAGGCAGCGGGACAAGGAAT
  AAGATGGACTTGGAAAACTGCCCAGACAAAGAAAACGAGAGAAGACAGAAGACGCCTTTCCAGGAGAATG
  ACGGTGATTCATTGCTCAAAACTAGAGAAGGTGGTCTGCCAGAGGAGCAGAGCCTAACTCCAAGTCCCTC
  GGCTAACCCTGAAATCTCCGTGTCAAAAGGAATCCATCAGGACCCGCAGCTGGAGGCAGAGGCTGGGGCC
  CCACACTGTGGTACAAACCCACAGCCAGCTCAGGATCCACCTCGGAATCAACAGATACCAAACCCACCAA
  CATCCACGTCGGAAGATGTGAAGCCCAAAACCTTGGCCCTGGAGAAAACCATCAATCATCAGATGGAGTC
  TCCGGGGGAAAGGCGGAAGTCTATAAGCGGGAAGAAACTGTGCTCCTCCTGCGGGCTCACTTTGGGTAAA
  GGTGCTGCGATGATCATCGAGACCCTGAATCTCTACTTTCACATTCAGTGTTTCAGGTGCGGCATCTGTA
  AAGGACAGCTCGGAGATGCAGTAAGCGGGACAGACGTCAGGATTCGCAATGGTCTCCTAAACTGTACCGA
  CTGCTACATGCGATCCAGAAGTGCCGGCCAGCCTACAACACTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001256122
• Insert Size: 2706 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001256122.1, NP_001243051.1
• RefSeq Size: 5904 bp
• RefSeq ORF: 2706 bp
• Locus ID: 77569
• UniProt ID: Q3UH68
• Cytogenetics: 5 C3.1
• Gene Summary: Actin stress fibers-associated protein that activates non-muscle myosin IIa. Activates the non-muscle myosin IIa complex by promoting the phosphorylation of its regulatory subunit MRLC/MYL9. Through the activation of non-muscle myosin IIa, positively regulates actin stress fibers assembly and stabilizes focal adhesions. It therefore negatively regulates cell spreading and cell migration.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.