C77080 (NM_001285867) Mouse Untagged Clone

CAT#: MC229139

C77080 (untagged) - Mouse expressed sequence C77080 (C77080), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: C77080
• Synonyms: Kiaa1522; mKIAA1522
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229139 representing NM_001285867
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGAGAGCTCCACCGCGGAGGATGCGCTCTCCGTCCGCTCGGAGATGATTCAGCGCAGAGGCTCCA
  CCTTCAGACCCCATGACTCATTTCCCAAATCTGGGAAGTCAGGAAGGCGACGGAGGGAGCGAAGGAGCAC
  GGTGCTGGGGCTGCCTCAGCACGTGCAGAAGGAGCTCGGCCTGCGGAACAACCGTGAGGCACCGGGCACT
  CCACAGCCTCCTGGTTCACGGGACGCTGTCCGCATCCCCACGGTGGATGGTCGCCCCGGTCTGGCCTTAG
  GGACAGGGGTCCGAGTGTCCCTGCAGGCTCTGGAAGCAGAAACAGAGGCCGGCACAGATGCAGAGGCTGT
  CATCCAGCGCCACATCGACCGTGTTTACCACGATGACACGCTTGTTGGCCGATCCACGGGAGCCCGGCCA
  CCGCCACTGACGAGGCCAATGTCTCTCGCAGTGCCTGGACTGACAGGAGGAGCAGGATCTCCAGAGCCAC
  TGAGCCCAGCCATGTCAATCTCACCCCAGGCCACCTACTTGTCGAAGCTGATCCCGCACGCCGTGCTGCC
  GCCCACCGTGGATGTGGTGGCCCTGGGCCGCAGCAGCCTGCGCACTCTGAGCCGTTGCAGCCTGCTCTCT
  GCCAGCCCAGCTTCGGTTCGCTCCCTGGGCCGCTTCTCCTCGGCTTCCAGTCCACGGCCCCGCAGCCGCA
  ACGCTTCCTCGTCCAGTGACAACTGGAGCCACTCTCAGTCCTCCGAGACCATTGTGTCTGATGGTTCCAC
  TCTTTCCTCTAAGGGGGGCTCTGAGGGCCAGCCAGAAGGCTCTGTAGCTAGCAATAATGTGGCGCCCCCT
  CCTCCAGGCGGTAGTGGGCGGGGCTCCCCCAGCGGGGGTAGCACTGCGGAGACCTCAGACACGGCCAGCA
  TCCGAAGCAGTGGGCAGCTGTCTGGCAGGAGTGTGTCCCTGCGTAAGATGAAACGGCCTCCCCCGCCTCC
  CCGCCGGACCTACTCCCTCCATCAGCGCGGCTCCGCAGTGCCTGATGGGCCCTTAGGGTTGCCGCCCAAA
  CCTGAGCGAAAGCAGCAGCCACAGCTGCCTCGGCCGCCCACTGCGGGTGGCTCTTCAGGGGTGGGGGCAG
  TATCTTGTCCACCCAGCTCAGCAGGCACCTGGGGCTCTGGCTTGTCCCCAGGTGGCTCCAGGCGTCCCCC
  ACGTTCCCCAGAACGGACACTTTCACCTTCAAGTGGCTACTCGAGCCAGAGCGGTACCCCAACTCTCCCT
  CCCAAGGGTCTGGCAGTTGCCCCTGCTTCCCCAGGCAAGGCTCAGCCCCCCAAACCAGATCGAGTGACAT
  CCCTTCGATCTCCTGGGGCCTCTGTATCCTCTTCCCTTACATCTCTGTGTTCCTCTTCTTCAGACCCTAC
  TCCTTTAGACCGCTCTGGCCCACAAATGTCTACCCCCTTGAGTGACAGGTTCGTCATACCTCCTCATCCC
  AAGGTGCCTGCTCCTTTCTCTCCACCACCTTCCAAGTCCAAGAGCTCGAACCAAGCTGCTCCTGTTCTGG
  CTGCCCCTGCTGTGGCTCCTGGGCAGGTGTCCACCATCGACACCAGTCCTGCATCCCCTTCCATGCCCCA
  GACAACCTTGACTCCAGCCCAGGAGTCTCCTGTTGCCTCCAAAGATGAGTCACCCCCACCATCCCCACCC
  CCATCTTACCATCCACCCCCACCACCTACTAAGAAGCCGGAGGTGCTGGAGGAGGCCCCACCTCCTCCGG
  AAGCTGCTGTGGAGATCCTTCCAGATCCCAGCTGGCCGCCGCCACCACCACCTGCACCTGAGGAACAGGA
  CCTGTCGATGGCTGACTTCCCCCCTCCTGAGGAGGTCTTCTTCAATGCAGGCCCTGAGCTTGGCCCTCTG
  GAGTCCTGCAGCTCTGAGGCTGCCGTCCCCCCAGCTGCTAGCTTGTCCCAGACTCCTCCGCCAGCTCCAC
  CTCCTAGTTCTGGATCAGAACCTCTGGCCAGGCTCCCACAGAAGGACTCAGTGGGCAAGCACAGCGGGGC
  TCCCAGGGAGGATTCGGGCACGCCTCTGGTCACACCCTCGCTCCTGCAGATGGTCCGGCTTCGCTCTGTG
  GGTGCTTCCACAGGGATTCCGAACCCTTCTCCGGGTTCATCGGCCCCTCAGAAGCCTCTGCGAAGAGCCC
  TGTCTGGGCGCGCCAGCCCAGTGACTGCTCCCTCCTCTGGGCTCCATGCTGCCGTCCGACTCAAGGCCTC
  TAGCCTGGCTGCCAGTGAGAGTCCTGCGAGTGCTCTGCCCACTGGAATACCCGAGGCAGAGCCACGGTCG
  CCACAGTCTCCTGCCTCCAAGGCCAGCTTCATCTTCTCCAAGGGCACCAAAAAACTGCAGCTGGAGAGGC
  CCGTGTCCCCCGAGGCCCAGGCTGACCTCCAGCGGAATCTGGTGGCTGAACTTCGGAGCATTTCAGAGCA
  TCGGCCACCTCCCCAGGCCCAGAAGAAGCCTTCCAAGGCTCCCCCACCAGTGGCCCGCAAACCCTCAGTG
  GGAGTCCCTCCTCCGTCCCCCAGTCTTCCCAGGACGGAGTCTCTTACTGCTCCATCCACCAATGGGCTCC
  CTCACGCTGAGGACAGGACTAACGGGGAGCTGGCGGAGAATGGAGGTGTGCAGCTGGCTGCTACAGAGAA
  GATGGGCTCCCCTGGTTCAGATCCACAGAAGAAACTGGTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001285867
• Insert Size: 2703 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285867.1, NP_001272796.1
• RefSeq Size: 4955 bp
• RefSeq ORF: 2703 bp
• Locus ID: 97130
• UniProt ID: A2A7S8
• Cytogenetics: 4 D2.2