Map4k3 (NM_001290345) Mouse Untagged Clone

CAT#: MC229130

Map4k3 (untagged) - Mouse mitogen-activated protein kinase kinase kinase kinase 3 (Map4k3)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Map4k3
• Synonyms: 4833416M01Rik; 4833416M07Rik; 9530052P13Rik; Glk; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229130 representing NM_001290345
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCCGGGCTTCGATCTGTCCCGCCGGAACCCGCAGGAGGACTTCGAGCTGATCCAGCGCATCGGCA
  GCGGCACCTACGGCGACGTCTACAAGGCACGGAATGTCAACACTGGAGAATTAGCAGCAATTAAAGTGAT
  AAAATTGGAACCAGGAGAAGACTTTGCGGTTGTACAGCAAGAAATCATTATGATGAAAGACTGCAAACAC
  CCAAACATTGTTGCTTATTTTGGAAGCTATCTCAGGCGAGATAAACTTTGGATTTGCATGGAATTCTGTG
  GAGGTGGCTCTTTACAGGACATATATCATGTAACTGGACCCCTTTCAGAACTGCAGATTGCATATGTTAG
  CAGAGAAACTCTACAGGGATTGTATTACCTTCACAGTAAAGGAAAAATGCACCGAGATATAAAGGGCGCT
  AACATTCTCCTAACGGATAATGGTCACGTGAAACTAGCTGATTTTGGAGTTTCTGCACAGATAACAGCTA
  CAATTGCCAAACGGAAGTCCTTCATTGGAACGCCATATTGGATGGCCCCAGAAGTCGCGGCTGTTGAGAG
  GAAAGGCGGGTACAATCAACTGTGTGACCTCTGGGCGGTGGGCATCACTGCCATCGAGCTGGCGGAGCTC
  CAGCCACCCATGTTTGACCTTCACCCGATGAGGGCATTGTTTCTAATGACAAAAAGCAATTTCCAGCCTC
  CTAAACTAAAAGATAAGTTGAAGTGGTCAAATAGTTTCCATCATTTTGTGAAGATGGCTCTTACCAAAAA
  TCCAAAGAAAAGACCCAATGCAGAAAAGCTGTTACAGCATCCCTTTGTCACACAGCCTCTGACACGGTCT
  TTGGCAATCGAGTTGCTGGACAAAGTGAATAATCCAGACCATTCCACATACCACGATTTTGATGATGATG
  ACCCAGAGCCTCTTGTTGCCGTACCACATAGAATTCCTTCAACGAGTAGAAATGTGAGAGAAGAAAAAAC
  ACGCTCAGAGATAAACTTTGGCCAGGTAAAATTTGATCCGCCCTTAAGGAAGGAGACAGAGCCACATCAC
  GAACTTCCCGACAGTGATGGTTTTTTCGACAGTTCAGAAGAAATATACTACACTGCAAGATCTAATCTGG
  ATCTGCAACTAGAGTACGGACAAGGACACCAAAGCCATTGCTTCTTAGGTGGCAACAAGAGTCTTCTGAA
  GTCTGTTGAAGAGGAATTACATCAGCGAGGACATGTGGCACACTTAGAAGATGACGAAGGCGACGATGAC
  GACTCTAAGCATTCAACCATGAAAGCAAAAGTCCCGCCTCCTCTGCCACCAAAGCCTAAATCCATCTTCA
  TACCACAGGATACACATTCTGCTGAGGACGGTAATCAAGGAACAATCAAGAGATGTCCCTCATCAGGGAG
  CCCGGCAAAGCCATCCCACGTTCCACCTAGACCACCTCCGCCCAGGTTACCGCCACAGAAGCCTGCTGTT
  TTAGGAAATGGAGTGAACTCCTTCCAGCTAAATGGTGAGCGAGACGGATCATTATATCAGCAGCAGAGTG
  AGCAAAGAGGCACAAACCTTTCAAGAAAGGAGAAGAAGGACGTCCCGAAACCAATTAGTAATGGTCTTCC
  CCCAACACCTAAAGTGCATATGGGAGCGTGTTTTTCAAAGGTTTTTAATGGATGTCCCTTGAAAATCCAC
  TGTGCCACATCCTGGATAAACCCAGACACGAGAGACCAGTACTTGATATTTGGCGCTGAAGAGGGCATTT
  ATACCCTTAATCTCAATGAACTTCATGAAACATCAATGGAACAGCTGTTCCCCCGCAGGTGTACGTGGCT
  GTATGTGATGAACAATTGCCTGCTGTCAGTCTCGGGCAAAGCCTCTCAGCTTTACTCCCACAACTTACCG
  GGGCTTTTCGATTATGCAAGACAGATGCAGAAGCTTCCAGTAGCCATCCCAGCACACAAGCTCCCCGACC
  GAATACTGCCCAGGAAATTTGCTGTATCAGCAAAGATTCCTGAAACCAAATGGTGCCAGAAGTGCTGCGT
  GGTGAGAAATCCTTACACGGGCCATAAGTACCTGTGCGGGGCGCTTCAGACCAGCATTGTTCTATTAGAA
  TGGGTTGAGCCAATGCAGAAATTTATGTTAATTAAGCACATAGAGTTTCCTATGCCGTGTCCACTCCGGA
  TGTTTGAGATGCTGGTGGTCCCTGAGCAGGAGTACCCTCTGGTCTGTGTTGGCGTCAGCAGAGGGCGAGA
  CTTCAACCAAGTGGTTCGGTTTGAGACGGTCAATCCCAATTCTACCTCGTCGTGGTTTACAGAGTCAGAT
  GCCCCGCAGACAAGCGTCACTCATGTGACCCAGCTGGAGAGAGACACCATCCTTGTGTGCTTGGATTGTT
  GTATAAAAATAGTCAATCTCCAAGGAAGGTTGAAATCCAGCAGGAAATTGTCATCAGAACTCACCTTCGA
  TTTCCAGATAGAGTCCATAGTTTGTCTACAAGACAGTGTGCTAGCGTTCTGGAAACACGGGATGCAAGGT
  AGAAGCTTTAGATCTAATGAGGTAACACAAGAAATTTCAGATAACACAAGAATCTTCAGACTCCTTGGAT
  CTGACAGGGTTGTGGTTTTGGAAAGTCGGCCAACTGATAACCCAACAGCAAATAGCAATTTATACATCTT
  GGCGGGTCACGAAAACAGCTACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290345
• Insert Size: 2685 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290345.1, NP_001277274.1
• RefSeq Size: 4176 bp
• RefSeq ORF: 2685 bp
• Locus ID: 225028
• UniProt ID: Q99JP0
• Cytogenetics: 17 E3
• Gene Summary: May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The exon combination of this RefSeq is inferred because this gene lacks full-length transcript support. This exon combination is supported by tiling of partial mouse transcripts and by full-length orthologous alignments. CCDS Note: This CCDS representation has an inferred exon combination because this gene lacks full-length transcript support. This representation is supported by tiling of partial mouse transcripts and by full-length orthologous alignments.