Dlg1 (NM_001252433) Mouse Untagged Clone

CAT#: MC229127

Dlg1 (untagged) - Mouse discs, large homolog 1 (Drosophila) (Dlg1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dlg1
• Synonyms: B130052P05Rik; Dlgh1; E-dlg/SAP97; mKIAA4187; SAP-97; SAP97
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229127 representing NM_001252433
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGTCCGGAAGCAAGATACCCAGAGAGCATTGCATCTGTTGGAAGAATATCGATCGAAACTAAGCC
  AAACTGAAGACAGACAACTCAGAAGTTCCATAGAGCGGGTTATTAACATATTTCAGAGCAACCTCTTTCA
  GGCTTTAATAGATATTCAAGAATTTTATGAAGTGACCTTACTTGATAATCCAAAATGTGTGGATCATTCA
  AAGCAGTGTGAACCAGTTCAGCCTGTGACTACTTGGGAGATTGCCAGCCTTCCAAGCACTGCCGTGACGT
  CAGAAACCCTGCCCGGCAGCCTTAGCCCTCCAGTAGAGAAATACCGGTATCAGGATGAAGAGGTACTTCC
  TCCTGAGCATATTTCTCCACAAGTCACAAATGAGGTGCTAGGTCCAGAACTGGTCCATGTCTCAGAGAAG
  AACCTGTCAGAGATTGAGAATGTCCATGGATTTGTTTCTCATTCTCATATCTCACCAATAAAGGCAAATC
  CTCCTCCAGTGCTGGTCAACACAGACAGCTTAGAGACACCAACTTATGTTAATGGCACTGATGCAGATTA
  TGAATATGAGGAAATCACACTTGAAAGGGGAAATTCGGGTCTTGGTTTCAGCATTGCAGGAGGTACAGAC
  AACCCACACATTGGAGATGACTCAAGTATTTTCATCACCAAAATTATCACAGGCGGAGCGGCTGCCCAGG
  ATGGAAGATTGCGGGTAAATGACTGTATACTGAGAGTAAATGAAGCAGACGTTCGTGATGTAACCCACAG
  CAAAGCAGTGGAGGCATTAAAAGAAGCTGGATCTATTGTGCGATTGTATGTGAAAAGGCGGAAGCCAGCA
  TCAGAAAAAATCATGGAAATAAAGCTCATTAAAGGTCCTAAAGGTCTTGGGTTCAGCATTGCTGGAGGTG
  TTGGAAATCAGCACATTCCTGGTGATAACAGCATCTATGTAACCAAAATAATTGAAGGAGGTGCAGCACA
  CAAGGATGGCAAGCTTCAGATTGGAGATAAGCTTCTAGCAGTGAACAGTGTGTGTTTAGAAGAAGTTACT
  CATGAAGAAGCAGTGACTGCCTTAAAGAATACATCTGATTTTGTTTATTTGAAAGTGGCAAAACCAACAA
  GTATGTATATAAATGATGGCTATGCACCACCTGACATCACTAATTCTTCTTCTCAATCTGTTGACAACCA
  TGTCAGCCCGTCCTCCTGCTTGGGCCAGACGCCAACGTCACCAGCCAGGTACTCACCCATTTCTAAAGCT
  GTGCTCGGAGATGACGAGATCACTAGGGAACCTAGAAAAGTTGTTCTTCATCGTGGCTCAACAGGACTTG
  GTTTTAACATTGTGGGAGGTGAAGATGGAGAAGGGATTTTTATCTCCTTCATCCTTGCTGGCGGACCTGC
  TGATCTAAGTGGAGAGCTCAGAAAAGGAGATCGCATCATATCGGTGAACAGTGTTGACCTCAGAGCTGCA
  AGTCACGAACAAGCAGCAGCTGCACTAAAGAACGCAGGCCAAGCCGTCACCATCGTTGCGCAATATCGAC
  CCGAAGAGTACAGTCGTTTTGAAGCTAAAATCCATGACTTACGGGAGCAGATGATGAATAGCAGTGTCAG
  TTCAGGGTCAGGGTCTCTTCGAACCAGCCAGAAGCGCTCCCTCTATGTCAGAGCCCTCTTTGATTATGAC
  AAGACTAAGGACAGCGGGCTTCCCAGTCAAGGACTGAACTTCCGCTTTGGAGACATCCTCCATGTCATCA
  ATGCTTCTGACGACGAGTGGTGGCAAGCCAGGCAGGTCACCCCAGACGGGGAGAGTGACGAAGTCGGAGT
  GATTCCTAGTAAACGAAGAGTTGAGAAGAAGGAACGAGCCCGATTAAAAACGGTCAAATTCAATTCTAAA
  ACAAGAGGAGATAAAGGGTCATTCAATGACAAGCGTAAAAAGAACCTCTTTTCCCGAAAATTTCCCTTCT
  ACAAGAACAAGGACCAGAGTGAACAGGAAACGAGTGATGCTGACCAGCACGTAACTTCTAATGCCAGCGA
  TAGTGAAAGTAGTTACCGTGGTCAAGAAGAATATGTTTTATCTTATGAGCCAGTGAATCAACAAGAAGTT
  AATTATACCCGACCAGTCATCATATTAGGACCTATGAAAGACAGAGTAAATGATGACTTAATCTCAGAAT
  TTCCTGACAAATTTGGATCCTGTGTCCCTCATACAACTAGACCGAAGCGTGACTATGAGGTGGATGGACG
  AGATTATCATTTTGTGACTTCAAGGGAACAGATGGAAAAGGATATTCAGGAGCATAAGTTCATTGAAGCC
  GGCCAGTATAACAACCATCTGTATGGGACGAGCGTGCAGTCCGTGCGAGCAGTGGCAGAGAAGGGCAAAC
  ATTGTATCCTTGATGTGTCTGGAAATGCCATAAAGAGGTTGCAGATTGCACAGCTTTATCCAATATCTAT
  TTTTATTAAACCCAAATCCATGGAAAATATCATGGAAATGAACAAGCGCCTAACAGAAGAGCAGGCCAGA
  AAAACATTTGAGAGAGCCATGAAGCTGGAGCAGGAGTTCACTGAGCATTTCACAGCTATTGTCCAGGGAG
  ACACGCTGGAGGACATTTACAACCAAGTGAAACAGATCATCGAAGAACAGTCTGGGCCTTACATCTGGGT
  CCCAGCGAAAGAAAAGCTATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001252433
• Insert Size: 2682 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252433.1, NP_001239362.1
• RefSeq Size: 4675 bp
• RefSeq ORF: 2682 bp
• Locus ID: 13383
• UniProt ID: Q811D0
• Cytogenetics: 16 22.4 cM
• Gene Summary: Essential multidomain scaffolding protein required for normal development. Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels (By similarity). Functional regulator of Kv1.5 channel (By similarity). May play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an exon and uses an alternate splice site in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.