Dag1 (NM_001276482) Mouse Untagged Clone

CAT#: MC229121

Dag1 (untagged) - Mouse dystroglycan 1 (Dag1), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dag1
• Synonyms: D9Wsu13; D9Wsu13e; DG; Dp71; Dp427
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229121 representing NM_001276482
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGTGGACAACTGGCTACTGCACCCCCTCTGGGGACAGACCTTTCTCCTCCTCCTGTCTGTGGCTG
  TGGCTCAGGCCCACTGGCCCAGTGAACCCTCAGAGGCTGTGAGGGACTGGAAGAACCAGCTTGAGGCGTC
  CATGCACTCAGTTCTCTCCGACTTCCAGGAGGCTGTTCCCACCGTGGTTGGCATTCCAGACGGTACGGCT
  GTTGTCGGGCGCTCATTTCGAGTGAGCATTCCAACGGATTTAATTGCCTCCAGTGGGGAGATCATCAAGG
  TGTCTGCAGCAGGGAAGGAGGCCTTACCGTCTTGGCTACACTGGGACCCACACAGTCATATTTTGGAAGG
  CCTTCCTCTTGACACTGATAAAGGTGTGCATTACATCTCAGTGAGTGCTGCACGCCTGGGAGCCAATGGA
  AGCCACGTCCCCCAGACTTCCAGTGTGTTCTCTATCGAGGTCTACCCTGAAGACCACAATGAGCCACAGT
  CTGTACGGGCAGCCTCATCAGACCCTGGTGAGGTAGTGCCATCTGCCTGTGCTGCTGATGAGCCAGTGAC
  TGTCCTTACAGTGATTCTGGATGCTGACCTCACCAAGATGACCCCAAAGCAAAGGATCGATCTGTTGAAC
  AGAATGCAGAGCTTCTCAGAAGTAGAACTTCACAACATGAAGTTGGTGCCTGTAGTGAATAATAGACTAT
  TTGACATGTCGGCCTTCATGGCTGGCCCAGGAAATGCAAAGAAAGTGGTAGAGAATGGGGCTCTCCTGTC
  CTGGAAACTAGGCTGCTCCTTGAACCAGAATAGCGTCCCTGACATCCGTGGTGTAGAAACCCCTGCTAGG
  GAGGGTGCTATGTCTGCCCAACTTGGTTATCCTGTGGTGGGTTGGCACATTGCCAATAAGAAGCCCACTC
  TCCCCAAACGACTCCGGAGGCAGATCCACGCCACACCTACACCTGTTACTGCCATTGGACCCCCAACCAC
  GGCCATTCAGGAGCCACCATCGCGGATAGTGCCTACGCCTACATCTCCAGCCATTGCACCTCCAACAGAG
  ACCATGGCTCCTCCTGTCAGGGATCCTGTTCCAGGGAAGCCCACGGTCACCATTCGGACGCGAGGTGCCA
  TTATTCAGACCCCAACTCTGGGCCCTATCCAGCCTACTCGGGTGTCAGAAGCTGGTACCACGGTTCCTGG
  CCAGATTCGCCCAACACTGACAATTCCTGGCTATGTAGAGCCCACAGCCGTTATTACTCCTCCAACAACT
  ACCACAAAGAAGCCACGAGTGTCCACGCCAAAGCCAGCAACGCCTTCAACTGATTCGTCAACTACCACAA
  CTCGGAGGCCAACCAAAAAACCACGGACACCCCGACCAGTGCCCCGAGTCACCACCAAAGCACCCATCAC
  CAGGTTGGAGACAGCTTCCCCACCCACTCGAATCCGTACTACCACCAGTGGAGTGCCCCGTGGGGGAGAA
  CCTAACCAGCGGCCAGAGCTCAAGAATCACATTGACAGGGTAGATGCCTGGGTGGGAACCTATTTTGAGG
  TAAAGATTCCATCAGACACCTTCTATGACAATGAGGATACCACTACCGACAAGCTCAAGCTGACCCTGAA
  GCTTCGAGAGCAGCAGTTAGTAGGTGAGAAATCGTGGGTTCAGTTTAACAGCAACAGCCAGCTCATGTAT
  GGCCTGCCTGACAGCAGCCATGTGGGAAAACATGAGTATTTTATGCATGCCACAGACAAAGGGGGCCTCT
  CCGCTGTGGATGCCTTCGAGATCCATGTTCACAAGCGCCCACAAGGGGACAAGGCTCCTGCACGGTTCAA
  GGCCAGGCTTGCAGGGGATCCAGCACCGGTGGTGAATGACATTCACAAGAAAATTGCTTTGGTAAAGAAG
  CTAGCTTTTGCTTTTGGGGATCGAAACTGCAGCTCCATCACCCTTCAGAACATCACTCGGGGCTCTATCG
  TGGTGGAATGGACCAACAACACTCTGCCCCTGGAGCCCTGCCCCAAGGAGCAGATCATAGGGCTGAGCCG
  CAGGATTGCTGATGAAAATGGGAAGCCTCGTCCTGCCTTCTCCAATGCTCTGGAGCCTGACTTTAAGGCT
  CTGAGTATTGCTGTGACGGGCTCTGGCAGTTGTCGGCACCTCCAGTTTATCCCTGTGGCACCACCCTCTC
  CTGGAAGCTCAGCTGCACCAGCCACAGAGGTTCCAGACAGGGACCCCGAGAAGAGCAGTGAGGACGATGT
  TTACCTGCACACCGTTATCCCAGCCGTGGTGGTCGCGGCCATCCTGCTCATTGCTGGAATCATTGCTATG
  ATCTGCTATCGCAAGAAGAGGAAGGGCAAGCTGACCCTTGAGGACCAGGCCACCTTTATTAAGAAGGGGG
  TGCCTATCATCTTTGCGGATGAGCTGGATGACTCTAAGCCCCCGCCCTCTTCCAGCATGCCGCTCATCTT
  GCAGGAAGAGAAGGCTCCCCTCCCACCTCCTGAGTACCCCAACCAGAGTATGCCCGAGACCACTCCTCTG
  AACCAGGACACTGTGGGAGAGTACACACCCCTGCGGGATGAGGATCCTAACGCACCTCCCTATCAGCCAC
  CCCCACCCTTCACGGCTCCCATGGAGGGCAAGGGCTCCCGTCCCAAGAACATGACCCCATACCGATCACC
  CCCTCCGTATGTTCCCCCTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001276482
• Insert Size: 2682 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001276482.1, NP_001263411.1
• RefSeq Size: 5447 bp
• RefSeq ORF: 2682 bp
• Locus ID: 13138
• UniProt ID: Q62165
• Cytogenetics: 9 59.08 cM
• Gene Summary: This gene encodes dystroglycan, a central component of dystrophin-glycoprotein complex that links the extracellular matrix and the cytoskeleton in the skeletal muscle. The encoded preproprotein undergoes O- and N-glycosylation, and proteolytic processing to generate alpha and beta subunits. A complete lack of the encoded protein in mice results in embryonic lethality due to the disorganization of Reichert's membrane. Chimeric mice deficient in the encoded protein overcome embryonic lethality but develop a progressive muscular dystrophy. Alternative splicing results in multiple transcript variants, all encoding the same protein. [provided by RefSeq, Nov 2015]
  Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1-8 encode the same protein.