Zfp473 (NM_001289839) Mouse Untagged Clone

CAT#: MC229114

Zfp473 (untagged) - Mouse zinc finger protein 473 (Zfp473), transcript variant 5

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Zfp473
• Synonyms: D030014N22Rik; zfp-100; Znf473
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229114 representing NM_001289839
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGGAAGGAGGACGACCTTAAAGGAGGTTGCTGGAATCAGCCAGCTGTGGCTGAGGAGTTTGAGA
  CCCTCAAGGATTTAGCCATGGATTTCACCGTGGAAGACTGGAAAGACCTGGAGTCAGAGTGGGACCAGAG
  AGACCTGTTCTGGGATGTGACGCTGAACCACTACCAGGACATGTTCTCATTTAATACTTCGCAACCCAGC
  CTGACTTCCCAGCCAGATGTCAGGGAAGAGCTGGAAGCCACCAGTACTGAAGTGCCTGAGACCAAGAGCT
  CTCCTTTGCAGAGTGGTTTCGTAGAAGAAGACTTCTCCCAGATTATGGAGATATTTTCCAACGGGCAACT
  CAACTTTGAAGCCTGTATAGGTGAGGATTGGCTAAATAGTTTTCTCGGAGATCCAGAAAGTCTTCCAAGA
  CCTGACATCAGCGACAAGGAAAGCCCCGCGGATCATCAGAGTCCTGAGTCCAAGAGCGGCCTCAGTCCTG
  GGCCTCCCCTTTGCACAAGAGAGGATGCTGTGATGTCTGCTAGTCCTGAAAAGACCCTCACTCCAGTGAT
  ACTGAAGGAATCCAGGAGTGACCTGAGCCAGGAGGACTCTGTCCAGGGACACGAGAAGCCATATAAGTGT
  AGTGAGTGTGGGGAAAGCTTCAGCCAGAGTCACCACCTCATCCAGCACTGGGTTCTCCACACCTCAGGGG
  AGCCTCCTATATGGCGAGAGCAGCAGCGAGGTCTCAGCCAGGGTGCTCACTTTCCCATGTGTCCAGGGAC
  TCCAGCAAGCTATGAATCCTACACGTGTCAGGAGTGTGGGAAAAGGTTTAGTCAGAATGTGTACCTCCAG
  TGGCATCAGAAAATCCACACTGGAGAAAAACTTTGTAAAACTCAAAGTGACAGCAACCTAGAAGGGCTCT
  CTAGGAGTCCGAGTGTCGAGCCAGGAAAGCAGCGGCTGAGTAAAGACACTGACTCAGCAAAACCGAGTAC
  CATTCACGGTCAGGACCAGGAAAAACCACCTACTGGTGAAAGTCGTGACCAAGAAAACCTGCATGAGAGC
  CAGCCTGGTGACAGGCCATCCGTTCTTCATCCAAAGCCTCTTAGGCATCAGAAGACTCCTACAAACGCTA
  AGTGCTTCAGATGTAAGAAGTGTGGGGAGACATTCAGTGGGGCCTTTCATCTAGCTAAACACCAGAGAGC
  CCATGCTCAGAGACTCTACAAGTGCGCTTCGTGCCCTGCCGTCTTCAACCTAAGCAAGCATTGCTTCCAG
  CATCGCAAGTCACATTTCCCCAGCGCGGCCTGCGAGTGTCAGGGATGCAGAAAGAGCTTTAATTGGCGGT
  CATCACTCATTAAGCACCAGGCTATTCACAAAGGAGAGAAGCCGTACAAGTGTGATGAATGTGGTAAAGC
  TTTCAACCACTCCTCCACCCTGAAGATCCACCAGAGGATCCACAGTGGACAGAAGCCTCACAAATGCAGT
  GAGTGCGGGAAAGCCTTCTGTCGGAGAACAGATCTTACTGAGCATCAGCGAGTCCACTCGGGCTTCAGAC
  CCCATCAGTGTCCCGTGTGTGCCAGGACTTTTAACCGACCTTCACACTTAGTTCGACACCGGCTGAGACA
  TGCTGAAGAAAGGCACTTTGGCTGTGCTAAATGCAAGGAGACCTTCATCTATAAAGAACAGCTAGAGCGG
  CACAATAAAATCCACACCATCGAGGGCTTGTATGAGTGTAAGCAGTGTGGAGAACACTTCATTTGCAGGT
  CAACTCTGAATTGCCACCTGAGCATACACATTAGAGAAAATACAAGTGAGAAAGTTGTGGGTCAGAACTC
  ACAGCACACTGAAAAATGCTTTAAGAATACCAAGTGTAGAAAAGCCCCTAACCACAGCAGATACCTAGGG
  CAGCATGAGAAGATTCATGCCCAGGTGACATCCGGTGAGTGTGACCCATGTGGGGAAACCTATGACCAGA
  GTGTGCAGCCCATTTGCCATCAGAGCATCTGTGCTGGTGTGAAACCAAGTGAATGCGCTGAGCCTGAGAA
  ATGCACGCGAAACACCTCTGCCAGTGAGCACCATCCTTCCCAAAGGGAACCATCCTTCAAGTGTGACATA
  TACAACAGGGCCTTCAAACAGCGTGCCCACCTTTCAAAACATCAGTTGATTCACATCACTGAGAAGCCCT
  TTAAATGCAACGAATGTGACAGAGCCTTCAAGCAGAGTAACTACCTCATTCAGCATCAGAAGACTCACAC
  TGCAGAGAAGCACTTTGAATGTAGCGAGTGTGGGAAAACATTTCATCAGCGATCATGCCTTTCTAAGCAC
  CAGAAAATCCACTCAGGGGAGAAGCCCTTTAAGTGTGGTGACTGTGGGAAAGCCTTCATCTCTGGTGCCC
  AGCTCATCCGACACCAGAGAATTCACACTGGGGAGAAGCCGTACGTCTGCCAGGAATGTGGGAAAACCTT
  CAGCCAGAGCTCGTGCCTTACCCTTCACCTTAGAATTCACACTGGGGAGAAGCCGTACACATGCGGCACG
  TGCGGGAAAGCCTTTGCTCAGAGAGCAAACCAGAGGAAGCACGAGAGAATTCACACTGGGGAGAAGCCCT
  ATGCCTGCGGGCTGTGTGGGAAGGCCTTCGGCCTCCGCACCCATCTCCAGCAACACCAGAGAATTCACAC
  CAAGGCCAAACCATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289839
• Insert Size: 2676 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289839.1, NP_001276768.1
• RefSeq Size: 3580 bp
• RefSeq ORF: 2676 bp
• Locus ID: 243963
• UniProt ID: Q8BI67
• Cytogenetics: 7 B3
• Gene Summary: Involved in histone 3'-end pre-mRNA processing by associating with U7 snRNP and interacting with SLBP/pre-mRNA complex. Increases histone 3'-end pre-mRNA processing but has no effect on U7 snRNP levels, when overexpressed. Required for cell cycle progression from G1 to S phases (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (5) differs in the 5' UTR and uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. Variants 4 and 5 encode the same isoform (2), which is shorter than isoform 1.