Enpp2 (NM_001285994) Mouse Untagged Clone

CAT#: MC229107

Enpp2 (untagged) - Mouse ectonucleotide pyrophosphatase/phosphodiesterase 2 (Enpp2), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Enpp2
• Synonyms: AT; ATX; Auto; E-NPP 2; lysoPLD; N; Npps2; Pd; PD-; PD-Ialpha; Pdnp2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229107 representing NM_001285994
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAGACAAGGCTGTTTCGGGTCATACCAGGTAATATCCTTGTTCACTTTTGCCATCGGCGTCAATC
  TCTGCTTAGGATTCACAGCAAGTCGAATTAAGAGGGCCGAATGGGATGAAGGACCTCCCACAGTGTTATC
  TGACTCTCCATGGACCAACACATCTGGATCCTGCAAAGGTAGATGCTTTGAGCTTCAAGAGGTTGGACCT
  CCTGACTGTCGGTGTGACAACCTATGTAAGAGCTACAGCAGCTGCTGCCATGATTTTGATGAGCTCTGTT
  TGAAAACAGCTCGAGGCTGGGAGTGCACCAAAGACAGATGTGGGGAAGTACGAAATGAGGAAAATGCCTG
  TCACTGCTCAGAAGACTGCTTGTCCCGGGGAGACTGCTGTACCAACTACCAAGTGGTCTGCAAAGGAGAA
  TCACACTGGGTAGATGATGACTGTGAAGAAATAAGAGTCCCCGAATGCCCTGCAGGGTTTGTCCGCCCTC
  CGTTAATCATCTTCTCTGTGGATGGATTCCGTGCATCGTACATGAAGAAAGGCAGCAAGGTTATGCCCAA
  CATTGAGAAACTGCGGTCCTGTGGCACCCATGCTCCCTACATGAGGCCTGTGTACCCTACAAAAACCTTC
  CCTAATCTGTATACGCTGGCCACTGGTTTATATCCAGAATCCCATGGAATCGTTGGCAATTCAATGTATG
  ACCCTGTCTTTGATGCTACTTTCCATCTTCGAGGGCGAGAGAAGTTTAACCATAGATGGTGGGGAGGCCA
  ACCGCTATGGATTACAGCCACCAAGCAAGGGGTGAGAGCCGGGACATTCTTTTGGTCTGTGAGCATCCCT
  CACGAGCGGAGAATCCTAACTATCCTTCAGTGGCTTTCCCTGCCAGACAATGAGAGGCCTTCAGTTTATG
  CCTTCTACTCCGAGCAGCCTGATTTTTCTGGACACAAGTACGGCCCTTTTGGCCCTGAGATGACAAATCC
  TCTGAGGGAGATTGACAAGACCGTGGGGCAGTTAATGGACGGACTGAAACAACTCAAGCTGCACCGTTGT
  GTGAATGTTATCTTTGTTGGAGACCATGGAATGGAAGACGTGACATGTGACAGAACTGAGTTCTTGAGCA
  ACTATCTGACTAACGTGGATGATATTACTTTAGTACCTGGAACTCTAGGAAGAATTCGACCCAAGATTCC
  CAATAATCTTAAATATGACCCTAAAGCCATTATTGCTAACCTCACGTGTAAAAAACCAGATCAGCACTTT
  AAGCCTTACATGAAACAGCACCTTCCCAAACGTTTGCACTATGCCAACAATCGGAGAATCGAGGATCTCC
  ATTTATTGGTGGAACGCAGATGGCATGTTGCAAGGAAACCTTTGGACGTTTATAAGAAGCCGTCAGGAAA
  ATGTTTTTTCCAGGGTGACCACGGCTTTGATAACAAGGTCAATAGCATGCAGACTGTTTTTGTAGGTTAT
  GGCCCAACTTTTAAGTACAGGACTAAAGTGCCTCCATTTGAAAACATTGAACTTTATAATGTTATGTGCG
  ATCTCCTAGGCTTGAAGCCAGCTCCCAATAATGGAACACATGGAAGTTTGAATCACCTGCTACGCACAAA
  TACCTTTAGGCCAACCCTACCAGAGGAAGTCAGCAGACCCAATTACCCAGGGATTATGTACCTTCAGTCT
  GATTTTGACCTGGGCTGCACCTGTGATGATAAGGTAGAGCCAAAGAACAAATTGGAAGAACTAAATAAAC
  GCCTTCATACCAAAGGATCTACAGAAGCTGAAACCGGAAAATTCAGAGGCAGCAAACATGAAAACAAGAA
  AAGCCTTAATGGAAATGTTGAGCCTAGAAAAGAGAGACATCTCCTGTATGGACGACCTGCAGTGCTTTAT
  CGGACTAGCTATGATATCTTATACCATACGGACTTTGAAAGTGGTTACAGTGAAATATTCTTAATGCCTC
  TCTGGACTTCTTATACCATTTCTAAGCAGGCTGAGGTCTCTAGCATCCCAGAGCACCTGACCAACTGTGT
  TCGCCCTGATGTCCGTGTATCTCCTGGATTCAGTCAGAACTGTTTAGCCTATAAAAATGATAAACAGATG
  TCCTATGGATTCCTTTTTCCTCCCTATCTGAGCTCTTCCCCAGAAGCGAAATATGATGCATTCCTTGTAA
  CCAACATGGTTCCAATGTACCCTGCCTTCAAACGTGTTTGGACTTATTTCCAAAGGGTCTTGGTGAAGAA
  ATATGCGTCAGAAAGGAATGGGGTCAACGTAATAAGTGGACCGATCTTTGACTACAATTACAATGGCTTA
  CGTGACATTGAGGATGAAATTAAACAGTATGTGGAAGGCAGCTCTATTCCTGTCCCTACCCACTACTACA
  GCATCATCACCAGCTGCCTGGACTTCACTCAGCCTGCAGACAAGTGTGATGGTCCTCTCTCTGTGTCTTC
  TTTCATCCTTCCTCACCGACCTGACAATGATGAGAGCTGTAATAGTTCCGAGGATGAGTCGAAGTGGGTA
  GAGGAACTCATGAAGATGCACACAGCTCGGGTGAGGGACATCGAGCATCTCACCGGTTTGGATTTCTACC
  GGAAGACTAGCCGTAGCTATTCGGAAATTCTGACCCTCAAGACATACCTGCATACATATGAGAGCGAGAT
  TTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001285994
• Insert Size: 2664 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285994.1, NP_001272923.1
• RefSeq Size: 3536 bp
• RefSeq ORF: 2664 bp
• Locus ID: 18606
• UniProt ID: Q9R1E6
• Cytogenetics: 15 D1
• Gene Summary: This gene encodes a member of the phosphodiesterase and nucleotide pyrophosphatase family of bifunctional enzymes that hydrolize phosphodiester bonds of various nucleotides. The encoded protein undergoes proteolytic processing to generate a mature protein with lysophospholipase D activity, catalyzing the cleavage of the choline group from lysophosphatidylcholine to produce lysophosphatidic acid. This gene is expressed in numerous tissues and participates in neural development, obesity, inflammation and oncogenesis. A complete lack of the encoded protein in mice results in aberrant vascular and neuronal development leading to embryonic lethality. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate the mature protein. [provided by RefSeq, Sep 2015]
  Transcript Variant: This variant (3, also known as gamma) lacks an in-frame exon and contains an alternate in-frame exon in the central coding region, compared to variant 1. The encoded isoform (3) is shorter, compared to isoform 1. This isoform (3) may undergo proteolytic processing similar to isoform 2.