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Osbpl5 (NM_001199227) Mouse Untagged Clone

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SKU
MC229092
Osbpl5 (untagged) - Mouse oxysterol binding protein-like 5 (Osbpl5), transcript variant 2
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ORF Plasmid:
Tag Free DDK
  $881.00
4 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Osbpl5
Synonyms 1110006M06Rik; AI462538; Obph1; ORP5; Osbp2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC229092 representing NM_001199227
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGAGGAGGCCTTTCTCCGGCGCCGTTTCTCGCTGTGTCCACCAGCTTCTACTCCACAGAAGACTG
ACCCCCGGAAAGTCCCACGGAACCTGCTTCTGGGCTGCGAAAATGAGCTTGGTCCCATCACCCCAGGAAG
GGACATGGAGTCCAACGGCCCATCACAACCCAGAGATGAAGAGCCCCAGACCCCAGGCTCCGCCACGAAG
GTGCCACTGGCTGAGTACAGGCTATGCAATGGATCTGACAAGGAATGTACCTCTCCAACGACCAGGGTCT
CCAAGAAGGATGCTCTCAAGGCACAGAAGGAGAACTATCGGCAGGAGAAGAAGCGGGCCACGAAGCAGCT
GTTCAGTGCCCTGACTGACCCCAGTGTGGTCATCATGGCTGACAGCCTAAAGATCCGAGGGACCCTGAAG
AGCTGGACGAAGCTGTGGTGTGTACTGAAGCCAGGGGTGCTGCTCATTTACAAGACACCCAAGGTGGGCC
AGTGGGTGGGCACGGTGCTGCTGCACTGCTGCGAGCTTATTGAGCGCCCCTCCAAGAAGGACGGCTTCTG
CTTCAAACTCTTCCACCCGCTGGACCAGTCTGTGTGGGCTGTGAAGGGCCCCAAAGGCGAGAGTGTGGGC
TCCATCACTCAGCCCCTCCCTAGCAGCTACCTGATCTTCAGGGCCGCTTCTGAATCAGACGGCCGCTGCT
GGCTAGATGCCCTGGAGCTGGCCCTTCGCTGCTCCAGCCTCCTGCGACTGAGCACGTGCAAGCAGGGTCG
AGATGGGGAGCAGGGGTCCTCGCCTGATGCCTCTCCCTCTTCTCTCTACGGACTGCCCACCTCGGCCACC
ATCCCAGATCAAGACCTGTTCCCGCTGAATGGGTCTGCCTTGGAGAACGATGCATTCTCAGACAAGTCAG
AACGAGAGAATGCAGAAGACTCGGATGCCGAGACCCAGGATCACAGCCGCAAGACCAACGAGAGTGGGAG
CGACCTGCTAGACAGCCCTGGTGGGCCATGGAGGGGAACTACCTATGTGGAACAGGTCCAGGAGGAGCTG
GGAGAGCTGGACGAGACTTCCCAGGTGGAGACAGTGTCAGAGGAAAACAAGAGCCTGATGTGGGTGCTGC
TGCGACAGCTGCGGCCAGGCATGGACCTGTCCCGTGTGGTGCTGCCGACCTTCGTGTTGGAGCCTCGGTC
CTTCCTCGGCAAGCTCTCTGATTACTACTACCACGGAGACCTGCTCTCCAGGGCAGCAGCAGAGGATGAC
CCCTACTGCCGCATGAAGCTTGTGCTCCGGTGGTACCTGTCTGGCTTCTATAAGAAGCCCAAGGGCATCA
AGAAACCCTATAATCCCATTCTGGGGGAGACTTTTCGCTGCCGCTGGCTGCACCCTCAGACCAACAGTCA
CACCTTCTACATAGCAGAACAGGTGTCTCACCATCCTCCTGTGTCTGCCTTCTATGTCAGCAACAGGAAA
GATGGCTTCTGCATGAGTGGCAGCATCACAGCAAAGTCTAAGTTCTATGGGAACTCATTGTCGGCGCTTC
TGGACGGCAAGGCTAAACTCACCTTCCTTAATCGCAAGGAGGAGTATACCCTCACCATGCCCTATGCCCA
CTGCCGAGGGATCCTCTACGGCACCATGACCATGGAGCTGGGAGGCAAAGTGAACATCGAGTGTGAGAAG
AACAACCTCCAAGCTGAACTGGACTTCAAGCTCAAGCCTTTCTTTGGAAGCAGCGCCAACATCAACCAGA
TTTCCGGGAAGATCATGTCAGGAGAGGAAGTCCTGGCACGCCTCACTGGACACTGGGACAGAGATGTATT
TATCAAGGAAGAGAGCAGTGGAGGCACCGAGCTCTTCTGGACTCCGAGTGAGGAGGTCCGCAGGCAGCGG
CTGAAGCGGCACACAGTGCTGTTGGAAGAGCAGAGTGAACTGGAGTCTGAGAGGCTGTGGCAGCATGTCA
CCAGGGCCATCCGTGAGGGTGACCAGCACAAAGCCACACAGGAGAAGTCTGTGCTGGAAGAGGCGCAGCG
GCAGCGTGCCCGTGAGCACCAGCAGAGCCTCACTCCATGGAAGCCACAGTTGTTCTTACTGGACCCTCTC
ACTCAGGAATGGCGCTACCGATATGAGGACCTCAGCCCCTGGGACCCGCTGAAGGACATTGCCCAATATG
AGCAAGATGGGATTCTTCATACTCTACAGAGGGAGACTATGTCTGGCCAGACAACCTTTCTGGGGAGTCC
TGACTCCAGGCACAAGAGGCCTAGCTCTGATCGGCGGCTCCGCAAAGCCAGTGACCAGCCCTCTGGCCAT
AGCCAGGTCACTGAGAGCAGCGGCTCCACACCTGAGTCCTGCCCAGACCTCTCTGATGAGGACTTTGTTC
CTGGTGGTGAGAGCCCATGTCCTCGGTGCAGGCGAGAGGTACACCGGCTAAAGATGCTACAAGAGGCCGT
GCTGTCCATTCAGGAAGCTCAGCAAGAGCTGCATAGGCACCTTTCGACCATGTTAAGCTCCACAGTGCGT
GCTGGGCAGGCGCCAGCCCCCAGCCTCCTGCAGAATCCCCGCTCCTGGTTCCTGCTCTGCATCTTCCTGA
CGTGTCAGCTATTCATTAACTATATCCTCAAATAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001199227
Insert Size 2625 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001199227.1, NP_001186156.1
RefSeq Size 3794 bp
RefSeq ORF 2625 bp
Locus ID 79196
UniProt ID Q9ER64
Cytogenetics 7 88.29 cM
Summary Lipid transporter involved in lipid countertransport between the endoplasmic reticulum and the plasma membrane: specifically exchanges phosphatidylserine with phosphatidylinositol 4-phosphate (PI4P), delivering phosphatidylserine to the plasma membrane in exchange for PI4P, which is degraded by the SAC1/SACM1L phosphatase in the endoplasmic reticulum. Binds phosphatidylserine and PI4P in a mutually exclusive manner. May cooperate with NPC1 to mediate the exit of cholesterol from endosomes/lysosomes. Binds 25-hydroxycholesterol and cholesterol.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) has a different exon at its 5' terminus which results in the use of a downstream in-frame translation initiation site, compared to variant 1. It encodes an isoform (2) with a shorter N-terminus, compared to isoform 1.
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Documents
Resources
"NM_001199227" in other vectors (1)
SKU Description Size Price
MR231303 Osbpl5 (myc-DDK-tagged) - Mouse oxysterol binding protein-like 5 (Osbpl5), transcript variant 2 10 ug
$589.00 $801.00

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