Dnm2 (NM_001253893) Mouse Untagged Clone

CAT#: MC229085

Dnm2 (untagged) - Mouse dynamin 2 (Dnm2), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dnm2
• Synonyms: b2b2159Clo; Dyn2; Udnm
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229085 representing NM_001253893
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAACCGCGGGATGGAAGAGCTCATCCCGCTGGTTAACAAGCTGCAGGACGCCTTCAGCTCCATCG
  GTCAGAGCTGCCACCTGGACCTGCCGCAGATCGCCGTGGTGGGCGGCCAGAGCGCCGGCAAAAGTTCGGT
  GCTCGAGAACTTCGTGGGCCGGGACTTCCTTCCACGAGGATCAGGAATTGTCACCCGGAGGCCTCTCATT
  CTGCAGCTCATCTTTTCCAAAACAGAATATGCGGAATTTTTGCACTGCAAGTCCAAAAAATTTACAGACT
  TTGATGAAGTCCGGCAGGAGATCGAAGCAGAGACTGACCGGGTCACAGGCACCAACAAAGGCATCTCCCC
  TGTGCCCATCAACCTTCGGGTCTACTCACCACACGTGTTGAACTTGACCCTCATCGACCTCCCAGGCATC
  ACTAAGGTGCCGGTGGGGGACCAGCCGCCAGACATCGAGTACCAGATCAAGGACATGATCCTGCAGTTCA
  TCAGCCGCGAGAGCAGCCTCATTCTTGCCGTCACACCTGCCAACATGGACTTGGCCAACTCAGACGCCCT
  CAAGCTGGCCAAGGAGGTGGACCCCCAAGGCCTACGGACCATCGGTGTCATCACCAAGCTAGACTTGATG
  GATGAAGGCACAGACGCCAGGGATGTCCTGGAAAACAAGCTACTGCCCTTGAGAAGAGGCTATATCGGCG
  TGGTTAACCGAAGCCAGAAAGACATCGAGGGCAAAAAGGACATCCGGGCTGCTCTGGCAGCCGAGAGGAA
  ATTCTTCCTCTCCCACCCAGCCTACCGGCACATGGCTGACCGCATGGGCACCCCACACTTGCAGAAAACC
  CTGAACCAGCAACTGACCAACCACATCCGAGAGTCACTGCCGACCCTTCGCAGCAAGCTGCAGAGCCAAC
  TGCTGTCCCTGGAGAAGGAAGTGGAAGAGTACAAGAATTTCCGGCCTGATGACCCCACGCGCAAGACCAA
  AGCCCTGCTGCAGATGGTTCAGCAGTTTGGAGTGGACTTTGAGAAGCGAATTGAAGGCTCGGGAGATCAA
  GTAGACACACTAGAGTTGTCTGGTGGAGCCCGCATCAATCGTATCTTTCATGAGCGCTTTCCCTTTGAAC
  TGGTAAAGATGGAGTTTGATGAGAAAGATCTACGAAGAGAGATCAGCTATGCTATTAAGAACATCCACGG
  AGTCAGAACTGGGCTCTTCACGCCGGATTTGGCGTTCGAGGCCATTGTGAAAAAGCAGGTGGTCAAGCTG
  AAAGAGCCCTGTCTGAAATGCGTGGACCTGGTTATCCAGGAGCTAATCAGTACAGTTAGGCAGTGCACCA
  GCAAGCTGAGTTCCTACCCCCGGCTGCGAGAGGAGACCGAGCGAATTGTCACCACCTACATCAGGGAGCG
  AGAAGGGAGAACCAAGGACCAGATTCTTCTTCTGATTGACATTGAGCAGTCGTACATCAACACAAACCAT
  GAAGACTTCATTGGATTTGCCAATGCCCAGCAGAGGAGCACGCAGCTGAACAAGAAGAGGGCCATACCCA
  ATCAGGGGGAGATCTTGGTGATCCGCAGGGGCTGGTTGACCATCAACAATATCAGCTTGATGAAGGGTGG
  CTCCAAGGAGTACTGGTTCGTGCTGACAGCTGAGTCATTGTCTTGGTACAAGGATGAGGAGGAGAAAGAA
  AAGAAGTACATGCTGCCACTGGACAACCTCAAGATCCGGGACGTGGAAAAGGGCTTCATGTCCAACAAGC
  ACGTGTTTGCCATCTTCAACACAGAGCAGAGGAATGTCTACAAGGACCTTCGACAGATTGAACTGGCTTG
  TGACTCCCAGGAAGATGTGGACAGCTGGAAGGCTTCGTTCCTGCGCGCTGGGGTCTACCCAGAGAAGGAC
  CAGGCAGAGAATGAGGATGGAGCACAAGAGAACACCTTCTCCATGGACCCGCAGCTGGAGCGACAGGTGG
  AGACCATCCGTAACCTGGTGGACTCCTACGTGGCCATCATCAACAAGTCCATCCGTGACCTTATGCCAAA
  GACCATCATGCACCTCATGATCAACAACACAAAGGCCTTCATCCACCATGAGCTGCTGGCTTACCTGTAT
  TCATCAGCAGACCAGAGCAGCCTCATGGAAGAGTCAGCCGAGCAGGCTCAGCGGCGGGACGACATGCTGC
  GCATGTACCACGCACTGAAGGAGGCCCTCAACATTATTGGGGACATCAGCACCAGCACCGTGTCCACACC
  CGTGCCCCCACCGGTCGATGACACGTGGCTCCAAAACACCAGCGGCCACAGCCCCACTCCACAGCGCCGA
  CCTGTGTCCAGTGTGCACCCACCAGGCCGGCCACCTGCAGTGCGGGGTCCTACACCGGGGCCTCCCCTGA
  TTCCTATGCCTGTGGGGGCCACATCCTCCTTCTCAGCACCTCCCATCCCATCCCGGCCTGGACCACAGAG
  TGTGTTTGCCAACAACGACCCCTTCTCTGCGCCACCTCAGATACCATCTCGGCCAGCACGGATTCCCCCT
  GGTATCCCTCCAGGAGTGCCCAGCAGAAGAGCGCCCGCTGCGCCCAGCCGGCCCACCATTATCCGCCCAG
  CCGAGCCGTCCCTGCTCGACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001253893
• Insert Size: 2613 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001253893.1, NP_001240822.1
• RefSeq Size: 3582 bp
• RefSeq ORF: 2613 bp
• Locus ID: 13430
• UniProt ID: P39054
• Cytogenetics: 9 7.79 cM
• Gene Summary: Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones (By similarity). Plays an important role in vesicular trafficking processes, in particular endocytosis. Involved in cytokinesis (PubMed:18923138). Regulates maturation of apoptotic cell corpse-containing phagosomes by recruiting PIK3C3 to the phagosome membrane (PubMed:18425118).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest variant and it encodes the longest protein (isoform 1).