Sulf1 (NM_001198565) Mouse Untagged Clone

CAT#: MC229083

Sulf1 (untagged) - Mouse sulfatase 1 (Sulf1), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sulf1
• Synonyms: AI467640; AW121680; mKIAA1077
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229083 representing NM_001198565
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTATTCCCTCTGGGCTCTGCTGCTTGCCGTGCTGGGCACACAGCTGCTGGGAAGCCTGTGTTCCA
  CCGTTCGGTCCCAGAGGTTCCGAGGAAGGATACAGCAGGAACGAAAAAACATCAGACCCAATATTATCCT
  TGTGCTCACTGACGACCAGGATGTGGAGCTGGGTTCCCTGCAAGTCATGAACAAGACGAGAAAGATCATG
  GAACAGGGTGGGGCCACCTTCACCAATGCCTTTGTGACCACGCCCATGTGCTGTCCATCACGCTCATCCA
  TGCTCACTGGGAAGTACGTGCATAACCACAATGTCTACACCAACAATGAGAACTGCTCGTCTCCCTCGTG
  GCAGGCAATGCACGAGCCTCGGACCTTTGCTGTGTATCTCAACAACACCGGCTACAGAACAGCCTTTTTT
  GGAAAATACCTCAATGAATACAATGGCAGCTACATCCCTCCTGGATGGCGAGAATGGCTCGGATTAATCA
  AGAATTCTCGTTTCTATAATTACACTGTTTGTCGCAACGGCATCAAGGAGAAGCATGGATTTGATTATGC
  AAAGGATTACTTCACAGACTTAATCACTAACGAGAGCATTAATTACTTCAAAATGTCTAAGAGAATGTAT
  CCCCATAGGCCCATTATGATGGTGATCAGCCATGCAGCACCACATGGCCCTGAAGACTCCGCCCCACAGT
  TTTCAAAACTGTACCCCAATGCCTCCCAACACATAACACCCAGCTATAACTATGCACCGAACATGGACAA
  ACACTGGATCATGCAGTACACAGGGCCCATGTTGCCTATCCACATGGAGTTCACCAACGTCCTCCAGCGC
  AAACGACTGCAGACTCTGATGTCAGTTGACGACTCTGTCGAGAGGTTGTACAACATGCTTGTGGAGAGCG
  GGGAGCTGGACAACACTTACATCATCTACACTGCGGACCACGGCTACCACATTGGACAGTTTGGGCTGGT
  CAAGGGGAAATCCATGCCCTACGACTTTGACATCCGTGTGCCTTTCTTCATTCGTGGTCCAAGCATAGAA
  CCAGGGTCGATAGTCCCACAGATTGTTCTGAACATCGACCTAGCTCCTACCATTCTGGATATTGCTGGTC
  TTGACTCTCCTTCTGATGTGGATGGTAAATCTGTCCTCAAACTTCTAGACCTGGAAAAGCCGGGTAACAG
  GTTTCGGACAAACAAGAAGGCCAAAATCTGGCGTGATACATTCCTAGTGGAAAGAGGGAAATTTTTACGG
  AAGAAGGAGGAATCCGGCAAGAATATCCAACAGTCAAACCACTTGCCCAAATATGAGAGGGTCAAGGAAC
  TGTGCCAGCAAGCCAGATACCAGACAGCCTGTGAGCAGCCTGGGCAGAACTGGCAGTGCATCGAGGACAC
  ATCCGGCAAGCTCCGAATCCACAAGTGTAAGGGACCCAGCGACCTGCTCACGGTCCGTCAGAATGCACGC
  AACCTCTACTCTCGCGGATTGCATGACAAAGACAAAGAGTGCCATTGTAGGGACTCTGGCTATCGCTCGA
  GCAGAAGCCAGAGAAAGAATCAAAGGCAGTTCCTGAGGAACAAGGGAACACCAAAGTATAAGCCCAGATT
  TGTGCACACCCGGCAGACTCGGTCCCTGTCTGTTGAATTCGAAGGTGAAATATATGACATCAACCTGGAA
  GAAGAAGAACTGCAAGTGTTACCACCAAGGAGCATTGCCAAGCGCCATGATGAGGGTCACCAGGGCTTCA
  TTGGCCACCAGGCTGCTGCTGGTGACATCAGGAATGAGATGTTGGCAGACAGCAACAATGCTGTGGGGTT
  ACCCGCCACTGTCCGGGTGACACACAAGTGCTTTATTCTTCCAAACGACACAATCCACTGTGAGAGGGAG
  CTGTACCAGTCAGCCAGAGCGTGGAAGGACCATAAGGCCTACATTGATAAAGAGATTGAAGTTCTACAAG
  ATAAAATTAAGAATTTAAGGGAAGTGAGGGGACACCTAAAGAAAAGGAAACCTGAGGAGTGTGGCTGTGG
  TGACCAGAGCTATTACAACAAAGAGAAAGGTGTCAAACGACAGGAGAAGCTAAAGAGTCACCTTCACCCC
  TTCAAGGAGGCTGCTGCCCAGGAGGTGGATAGCAAACTTCAGCTCTTCAAGGAGCATCGGAGGAGGAAGA
  AGGAGAGGAAGGAGAAGAAACGGCAGAGGAAGGGAGAGGAGTGTAGCCTGCCTGGCCTTACCTGCTTCAC
  CCATGACAACAACCACTGGCAGACTGCCCCATTCTGGAACTTGGGATCTTTCTGTGCCTGCACAAGTTCT
  AACAACAATACCTACTGGTGTTTGCGTACAGTCAACGAGACGCACAATTTCCTGTTTTGTGAGTTTGCTA
  CTGGCTTTCTGGAATATTTCGACATGAATACGGATCCTTATCAGCTCACAAATACAGTACACACAGTAGA
  ACGGAGCATCTTAAATCAGCTACACATACAGCTGATGGAGCTCCGAAGCTGCCAAGGGTATAAACAGTGC
  AACCCAAGACCCAAGAGCCTGGACATTGGAGCTAAAGAAGGAGGAAACTATGACCCGCACAGAGGACAGT
  TATGGGATGGATGGGAAGGTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001198565
• Insert Size: 2613 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001198565.1, NP_001185494.1
• RefSeq Size: 4639 bp
• RefSeq ORF: 2613 bp
• Locus ID: 240725
• UniProt ID: Q8K007
• Cytogenetics: 1 A3
• Gene Summary: Exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin. Diminishes HSPG (heparan sulfate proteoglycans) sulfation, inhibits signaling by heparin-dependent growth factors, diminishes proliferation, and facilitates apoptosis in response to exogenous stimulation (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) may represent the longest transcript. All three variants encode the same protein.